Membrane-Delimited Stimulation of Heart Cell Calcium Current by ß-Adrenergic Signal-Transducing Gs Protein

  • S. Pelzer
  • Y. M. Shuba
  • L. Birnbaumer
  • T. F. McDonald
  • D. J. Pelzer
Conference paper
Part of the NATO ASI Series book series (volume 60)

Abstract

A several-fold increase in calcium current (Ica) is a signal feature of the maximal beta-adrenergic response of the heart. It is generally ascribed to enhanced adenosine 3’,5’-cyclic monophosphate (cAMP)dependent phosphorylation of calcium (Ca) channels following beta-receptor activation of the guanosine nucleotide-binding (G) protein Gs, and Gs-activation of the adenylyl cyclase cascade. We blocked phosphorylation pathways in guinea pig ventricular cardiomyocytes to unmask other possible Ica-stimulatory modes. In blocked cells, Ica increased by approximately 50% during (i) beta-receptor activation of Gs, (ii) intracellular activation of Gs, and (iii) intracellular application of preactivated Gs. We conclude that fast, membrane-delimited Gs modulation participates in the physiological regulation of cardiac Ica. Additionally, membrane-delimited action by activated Gs seems to hinder Ca channel inhibition by D600 and may prime Ca channels for up-regulation by cAMP-dependent phosphorylation.

Key words

Cardiomyocytes-Calcium current regulation-Activated Gs protein 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • S. Pelzer
    • 1
  • Y. M. Shuba
    • 1
    • 2
  • L. Birnbaumer
    • 1
    • 3
  • T. F. McDonald
    • 1
  • D. J. Pelzer
    • 1
  1. 1.Department of Physiology and BiophysicsDalhousie UniversityHalifaxCanada
  2. 2.A. A. Bogomoletz Institute of PhysiologyUkrainian Academy of SciencesKiev 24USSR
  3. 3.Departments of Cell Biology and Molecular Physiology and BiophysicsBaylor College of MedicineHoustonUSA

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