Abstract
Meiotic crossing over within the I region of the mouse major histocompatibility complex (MHC) has been shown to be limited to a number of well-defined recombinational hotspots. Twenty cases of recombination mapping to the Eb gene of the I region have been sequenced to more precisely define the segment of DNA carrying meiotic crossover sites. This analysis was based on positioning recombination breakpoints between nucleotide polymorphisms found in the nucleotide sequences of parental haplotypes. Each of the twenty cases of recombination mapped to the second intron of the Eb gene. The exact sites of crossing over could not be determined in these recombinants since the nucleotide sequences of the parental alleles are identical between the polymorphic sites, and because no deletion or duplication of nucleotides was observed in recombinant sequences. These studies indicate that the second intron of the Eb gene contains at least two closely linked clusters of recombinational breakpoints or, alternatively, a broad area of approximately 800–1000 bp in which recombination can occur at multiple sites.
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References
Begovich, A.B. and Jones, P.P.: Free Ia Eα chain expression in the E- α:E- ĂŸ recombinant strain A.TFR5. Immunogenetics 22: 523–532, 1985
Bryda, E.C., Depari, J.A., Sant’Angelo, D.B., Murphy, D.B. and Passmore, H.C.: Multiple sites of crossing over within the Eb recombinational hotspot in the mouse. 1991 (Submitted)
Blin, N. and Stafford, D.W.: A general method for isolation of high molecular weight DNA from eukaryotes. Nucleic Acids Research 3: 2303–2308, 1976
Epstein, S.L., Am, J.S., Sachs, D.H.: Seven new MHC recombinant strains defining new H-2 haplotypes. Immunogenetics 32: 142–145, 1990
Kobori, J.A., Winto, A., McNicholas, J. and Hood, L.: Molecular characterization of the recombination region of six murine major histocompatibility complex (MHC) I-region recombinants. J Mol Cell Immunol 1: 125–131, 1984
Kobori, J.A., Strauss, E., Minard, K., and Hood, L.: Molecular analysis of the hotspot of recombination in the murine major histocompatibility complex. Science 234: 173–179, 1986
Lafuse, W.P. and David, C.S.: Recombination hot spots within the I region of the mouse H-2 complex map to the E ĂŸ and E α genes. Immunogenetics 24: 352–360, 1986
Lafuse, W.P., Berg, N., Savarirayan, S., and David, C.S.: Mapping of a second recombination hot spot within the I-E region of the mouse H-2 gene complex. J Exp. Med. 163: 1518–1528, 1986
Lafuse, W.P., Lee, S.T., and David, C.S.: Molecular analysis of two recombinant mouse strains with crossovers in the E α recombination hot spot. J Immunogenetics 17: 169–176, 1990
Passmore, H.C., Kobori, J.A., Zimmerer, E.J., Spinella, D.G. and Hood, L.: Molecular characterization of meiotic recombination within the major histocompatibility complex of the mouse: mapping of crossover sites within the I region. Biochemical Genetics 25: 513–526, 1987
Radman, M.: Mismatch repair and genetic recombination. In R. Kucherlapati and G.R. Smith (eds.). Genetic Recombination, pp. 169–191, American Society of Microbiology, Washington DC 1988
Saha, B.K. and Cullen, S.E.: Molecular mapping of murine I region recombinants: crossing over in the E gene. J Immunol 136: 1112–1116, 1986
Shiroishi, T., Hanzawa, N., Sagai, T., Ishiura, M., Gojobori, T., Steinmetz, M., and Moriwaki, K.: Recombinational hotspot specific to female meiosis in the mouse major histocompatibility complex. Immunogenetics 31: 79–88, 1990
Shiroishi, T., Sagai, T., Hanzawa, N., Gotoh, H., and Moriwaki, K.: Genetic control of sex- dependent meiotic recombination in the major histocompatibility complex of the mouse. EMBO J10: 681–686, 1991
Steinmetz, M., Minard, K., Horvath, S., McNicholas, J., Frelinger, J., Wake, C., Long, E., Mach, B., and Hood, L.: A molecular map of the immune response region from the major histocompatibility complex of the mouse. Nature 300: 35–42, 1982
Steinmetz, M., Stephan, D., and Fischer Lindahl, K.: Gene organization and recombination hotspots in the murine major histocompatibility complex. Cell 44: 895–904, 1986
Uematsu, Y., Kiefer, H., Schulze, R., Fischer-Lindahl, K., and Steinmetz, M.: Molecular characterization of a meiotic recombinational hotspot enhancing homologous equal crossing-over. EMBO J 5: 2123–2129, 1986
Zimmerer, E.J. and Passmore, H.C.: Structural and genetic properties of the E ĂŸ recombinational hot spot in the mouse. Immunogenetics 33: 132–140, 1991
Zimmerer, E.J., Ogin, E., Shreffler, D.C. and Passmore, H.C.: Molecular mapping of crossover sites within the I region of the mouse MHC: analysis of ten recombinant chromosomes. Immunogenetics 25: 274–276, 1987
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© 1991 Springer-Verlag Berlin Heidelberg
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Passmore, H., Sant’Angelo, D., Bryda, E. (1991). Molecular Dissection of the Eb Recombinational Hotspot in the Mouse. In: Klein, J., Klein, D. (eds) Molecular Evolution of the Major Histocompatibility Complex. NATO ASI Series, vol 59. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84622-9_39
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DOI: https://doi.org/10.1007/978-3-642-84622-9_39
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