Molecular Dissection of the Eb Recombinational Hotspot in the Mouse
Meiotic crossing over within the I region of the mouse major histocompatibility complex (MHC) has been shown to be limited to a number of well-defined recombinational hotspots. Twenty cases of recombination mapping to the Eb gene of the I region have been sequenced to more precisely define the segment of DNA carrying meiotic crossover sites. This analysis was based on positioning recombination breakpoints between nucleotide polymorphisms found in the nucleotide sequences of parental haplotypes. Each of the twenty cases of recombination mapped to the second intron of the Eb gene. The exact sites of crossing over could not be determined in these recombinants since the nucleotide sequences of the parental alleles are identical between the polymorphic sites, and because no deletion or duplication of nucleotides was observed in recombinant sequences. These studies indicate that the second intron of the Eb gene contains at least two closely linked clusters of recombinational breakpoints or, alternatively, a broad area of approximately 800–1000 bp in which recombination can occur at multiple sites.
KeywordsMajor Histocompatibility Complex Nucleotide Sequence Analysis Restriction Fragment Length Polymorphism Recombinational Breakpoint Region Recombinant
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