Flow Cytometry pp 413-420 | Cite as

Expert Assistance for Flow Immunophenotyping

  • Gary C. Salzman
  • Richard J. Beckman
  • Carleton C. Stewart
  • Sigrid J. Stewart
Conference paper
Part of the NATO ASI Series book series (volume 67)

Abstract

There is a serious data analysis bottleneck for flow immunophenotyping in the clinical laboratory when many parameters are required. Multiple tubes of cells are measured on the flow cytometer for each patient peripheral blood or bone marrow specimen. Each tube requires only a few minutes to measure five variates on each of 7000 or more cells. The data for each patient, however, may require 20 to 40 minutes to analyze with the manual methods now in common use. This paper describes the use of cluster analysis to replace the manual gating methods used to find the leukemic or other abnormal cell populations. It then discusses the use of an expert system to assist in describing the immunophenotype of the specimen. Since the literature on expert systems in cytometry has been reviewed recently (Salzman and Bartels, 1992), this article does not include such a review.

Keywords

Leukemia 

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Gary C. Salzman
    • 1
  • Richard J. Beckman
    • 1
  • Carleton C. Stewart
    • 1
    • 2
  • Sigrid J. Stewart
    • 1
    • 2
  1. 1.Los Alamos National LaboratoryLos AlamosUSA
  2. 2.Laboratory of Flow CytometryRoswell Park Cancer InstituteBuffaloUSA

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