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Flow Cytometry pp 227-241 | Cite as

Cytometrie Tout Terrain or Bush DNA Cytometry

  • Spencer C. Brown
Conference paper
Part of the NATO ASI Series book series (volume 67)

Abstract

This unconventional title derives partially from our subject matter being flow cytometry applied to plants, but principally from a proposition developed in the conclusion: namely, that an industrial application of cytometry in the diagnosis of plant ploidy levels will be vastly more economic when the cytometer can go to the plants — on a wheel-barrow if necessary — rather than the samples coming to the laboratory!

Keywords

Ploidy Level Plant Protoplast Somatic Cell Line Flow Cytometric Determination Plant Ploidy Level 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Arumuganathan K, Earle ED (1991) Nuclear DNA content of some important plant species. Plant Mol Biol Rep 9: 208–218CrossRefGoogle Scholar
  2. Arumuganathan K, Slattery JP, Tanksley SD, Earle ED (1991) Preparation and flow cytometric analysis of metaphase chromosomes of tomato. Theor Appl Genet 82: 101–111CrossRefGoogle Scholar
  3. Bennett MD, Smith JB (1975) Nuclear DNA amounts in Angiosperms. Philos Trans R Soc, Ser B 274: 227–274CrossRefGoogle Scholar
  4. Bennett MD, Smith JB, Heslop-Harrison JS (1982) Nuclear DNA amounts in Angiosperms. Proc R Soc Biol 216: 179–199CrossRefGoogle Scholar
  5. Berglund DL, Strobel S, Sugawara F, Strobel GA (1988) Flow cytometry as a method for assaying the biological activity of phytotoxins. Plant Sci 56: 183–188CrossRefGoogle Scholar
  6. Bergounioux C, Brown S (1989) Plant cell cycle analysis with isolated nuclei. In: Crissman HA, Darzynkiewicz Z (ed) Flow Cytometry, Academic Press, New York (Methods in Cell Biology, 33, 563 )CrossRefGoogle Scholar
  7. Bergounioux C, Brown SC, Petit P (1992) Flow cytometry and plant protoplast cell biology. Physiol Plant, in pressGoogle Scholar
  8. Bergounioux C, Perennes C, Brown SC, Gadal P (1988a) Nuclear RNA quantification in protoplast cell-cycle phases. Cytometry 9: 84–87PubMedCrossRefGoogle Scholar
  9. Bergounioux C, Perennes C, Brown SC, Sarda C, Gadal P (1988b) Relation between protoplast division, cell cycle stage and nuclear chromatin structure. Protoplasma 142: 127–136CrossRefGoogle Scholar
  10. Brown SC, Bergounioux C (1989) Plant flow cytometry. In: Yen A (ed) Flow Cytometry: Advanced research and clinical applications, vol II. CRC Press, Boca Raton Florida, p 195Google Scholar
  11. Brown, S, Bergounioux, C, Tallet, S, Marie, D (1991a) Flow cytometry of nuclei for ploidy and cell cycle analysis. In: Negrutiu I, Gharti-Chherti GB (ed) A laboratory guide for cellular and molecular plant biology. Birkhäuser Verlag, Basel, p 326Google Scholar
  12. Brown SC, Devaux P, Marie D, Bergounioux C, Petit P (1991b) Cytométrie en flux: Application à l’analyse de la ploïdie chez les végétaux. Biofutur 105 (October): 1–14 (Technoscope n°47)Google Scholar
  13. Brown SC, Renaudin JP, Prevot C, Guern J (1984) Flow cytometry and sorting of plant protoplasts: Technical problems and physiological results from a study of pH and alkaloids in Catharanthus roseus. Phyiol Vég 22: 541–554Google Scholar
  14. Chaput MH, Sihachakr D, Ducreux G, Marie D, Barghi N (1990) Somatic hybrid plants produced by electrofusion between dihaploid potatoes: BF15 (Hl), Aminca (H6) and Cardinal ( H3 ). Plant Cell Rep 9: 411–414Google Scholar
  15. Conia J, Muller P (1989) Isolation, classification, and flow cytometric sorting of plant chromosomes. In: Yen A (ed) Flow Cytometry: Advanced research and clinical applications, vol I. CRC Press, Boca Raton Florida, p 143Google Scholar
  16. Costich DE, Meagher TR, Yurkow EJ (1991) À rapid means of sex identification in Silene latifolia by use of flow cytometry. Plant Mol Biol Rep 9: 359–370CrossRefGoogle Scholar
  17. De Laat AM, Gohde W, Vogekzang MJ (1987) Determination of ploidy of single plants and plant populations by flow cytometry. Plant Breeding 99: 303–307CrossRefGoogle Scholar
  18. De Rocher EJ, Harkins KR, Galbraith DW, Bohnert HJ (1990) Developmentally regulated systematic endopolyploidy in succulents with small genomes. Science 250: 99–101PubMedCrossRefGoogle Scholar
  19. Deslauriers C, Powell AD, Fuchs K, Pauls KP (1991) Flow cytometric characterization and sorting of cultured Brassica napus microspores. Biochim Biophys Acta 1091: 165–172PubMedCrossRefGoogle Scholar
  20. Fahleson J, Dixelius J, Sundberg E, Glimelius K (1988) Correlation between flow cytometric determination of nuclear DNA content and chromosome number in somatic hybrids within Brassicaceae. Plant Cell Rep 7: 74–77CrossRefGoogle Scholar
  21. Fox MH, Galbraith DW (1990) Application of flow cytometry and sorting to higher plant systems. In: Melamed M, Lindmo T, Mendelsohn M (ed) Flow cytometry and sorting, 2nd edn. Wiley-Liss, New York, p 632Google Scholar
  22. Galbraith DW (1989) Analysis of higher plants by flow cytometry and cell sorting. Int Rev Cytol 116: 165–228CrossRefGoogle Scholar
  23. Galbraith DW, Harkins KR, Maddox JM, Ayres NM, Sharma DP, Firoozabady E (1983) Rapid flow cytometric analysis of the cell cycle in intact plant tissues. Science 220: 1049–1051PubMedCrossRefGoogle Scholar
  24. Gantet P, Hubac C, Brown SC (1990) Flow cytometric fluorescences anisotropy of lipophilic probes in epidermal and mesophyll protoplasts from water-stressed Lupinus albus L. Plant Physiol 94: 729–737PubMedCrossRefGoogle Scholar
  25. Godelle B, Cartier D, Marie D, Brown SC, Siljak-Yakovlev S (1990) Relationship between flow cytometric determination of nuclear DNA content and the amount of heterochromatin within the Crepis praemorsa complex. Biol Cell 70 (1–2): 28aGoogle Scholar
  26. Godelle B, Cartier D, Marie D, Brown S, Siljak-Yakovlev S (1992) Heterochromatin study reveals non-linearity of fluorometry for genomic base composition, pers. comm.Google Scholar
  27. Hammatt N, Lister A, Blackhall NW, Gartland J, Ghose TK, Gilmour DM, Power JB, Davet MR, Cocking EC (1990) Selection of plant heterokaryons from diverse origins by flow cytometry. Protoplasma 154: 34–44CrossRefGoogle Scholar
  28. Harkins KR, Galbraith DW (1987) Factors governing the flow cytometric analysis and sorting of large biological particles. Cytometry 8:60-70 Langlet O (1927) Svensk Bot Tids 21: 397–422Google Scholar
  29. Le Gall Y, Brown S, Marie D, Mejjad M, Kloareg B (1992) Quantification of nuclear DNA and GC% in marine macroalgae by flow cytometry of isolated nuclei. J Phycology in pressGoogle Scholar
  30. Lister A (1989) Flow cytometry for selection of plant cells in vitro. In: Dix PJ (ed) Selection of plant cells, VCH Verlagsgesellschaft, Weinheim, chpt 3Google Scholar
  31. Matine A (1990) Biosynthèse d’isoprenoïdes par des protoplastes et plastes isolés du fruit de calamondin. Université de Bordeaux I, Ph.D. thesisGoogle Scholar
  32. Perennes C, Bergounioux C, Gadal P (1990) Direct assessment of hormone stimulated transcription in protoplasts and isolated nuclei of Petunia hybrida. Plant Cell Rep 8: 684–686CrossRefGoogle Scholar
  33. Petit PX (1992) Flow cytometric analysis of Rhodamine 123 fluorescence during modulation of the membrane potential in plant mitochondria. Plant Physiol 98: 279–286PubMedCrossRefGoogle Scholar
  34. Petit PX, Conia J, Brown SC, Bergounioux C (1986) Cytométrie en flux et biotechnologies végétales. Biofutur 5 (November): 128–137Google Scholar
  35. Petit PX, Diolez P, de Kouchkovsky Y (1989) Flow cytometric analysis of energy transducing organelles: mitochondria and chloroplasts. In: Yen A (ed) Flow Cytometry, Advanced research and clinical applications, vol I. CRC Press, Boca Raton Florida, p 271Google Scholar
  36. Sangwan R, Bourgeois Y, Brown SC, Vasseur G, Sangwan-Norreel B (1992) Characterization of competent cells and early events of Agrobacterium mediated genetic transformation in Arabidopsis thaliana. Planta, in pressGoogle Scholar
  37. Schröder W, Petit PX (1990) Study of lectin-binding sites on the chloroplast envelope using flow cytometry analysis. Physiol Plant 79 (2, part 2): A59Google Scholar
  38. Sgorbati S, Levi M, Sparvoli E, Trezzi F, Lucchini G (1986) Static and flow cytometry of 4’,6-diamidino-2-phenylindole ( DAPI)-stained suspensions of nuclei released from fresh and fixed tissues of plants. Physiol Plantarum 68: 471–476CrossRefGoogle Scholar
  39. Sgorbati S, Sparvoli E, Levi M, Chiatante D (1989) Bivariate cytofluorimetric analysis of nuclear protein and DNA relative to cell kinetics during germination of Pisum sativum seed. Physiol Plantarum 75: 479–484CrossRefGoogle Scholar
  40. Sree Ramulu K, Dijkhuis P (1986) Flow cytometric analysis of polysomaty and in vitro genetic instability in potato. Plant Cell Rep 3: 234–237CrossRefGoogle Scholar
  41. Tagu D, Bergounioux C, Perennes C, Brown SC, Muller P, Gadal P (1987) Analysis by flow cytometry of the primary events of Petunia hybrida protoplast transformation. Plant Sci 51: 215–223CrossRefGoogle Scholar
  42. Ulrich I, Fritz B, Ulrich W (1988) Application of DNA fluorochromes for flow cytometric DNA analysis of plant protoplasts. Plant Sci 55: 151–158CrossRefGoogle Scholar
  43. Ulrich I, Ulrich W (1991) High-resolution flow cytometry of nuclear DNA in higher plants. Protoplasma 165: 212–215CrossRefGoogle Scholar
  44. Veuskens J, Marie D, Hinnisdeals S, Brown S (1992) Flow cytometry and sorting of plant chromosomes. In: Radbruch A (ed) EMBO Course Methods, Flow cytometry and cell sorting, Springer, Berlin Heidelberg New York (Monograph Series, Methods in biology and medicine), in pressGoogle Scholar
  45. Xu C, Auger J, Givindjee (1990) Chlorophyll a fluorescence measurements of isolated spinach thylakoids obtained by using single-laser-based flow cytometry. Cytometry 11: 349–358PubMedCrossRefGoogle Scholar
  46. Ye D, Oliveira M, Veuskens J, Wu Y, Installe P, Hinnisdaels S, Truong AT, Brown SC, Mouras A, Negrutiu I (1991) Sex determination in the dioecious Melandrium. The X/Y chromosome systems allows complementary cloning strategies. Plant Sci 80: 93–106CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1993

Authors and Affiliations

  • Spencer C. Brown
    • 1
  1. 1.Cytométrie Institut des Sciences VégétalesCentre National de la Recherche ScientifiqueGif-sur-YvetteFrance

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