Biochemical Analysis and Cellular Location of the GCTM-2 Antigen in Embryonal Carcinoma and in Other Tumour Cell Lines

  • M. D. Mason
  • M. F. Pera
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 123)


We have previously described the development of monoclonal antibody GCTM-2 from a detergent-insoluble extract of the human embryonal carcinoma (EC) cell line GCT 27 (Pera et al. 1988). Immunofluorescence studies showed that GCTM-2 strongly stained human EC cells in preparations of cells fixed in a 50 : 50 mixture of methanol and ethanol, and in preparations utilising live cells. This indicated that it was not necessary to permeabilise the cells by fixation in order to render the antigen accessible to the antibody. Electron microscopy studies localised the antigen outside the cell surface, and these along with the above data and the resistance of the antigen to extraction with high salt and non-ionic detergent from a monolayer culture of EC were compatible with the hypothesis that the antigen was located in the pericellular matrix. It was also observed that the antigen disappeared from the cell surface during the spontaneous differentiation of human EC cells in vitro (Pera et al. 1989).


Embryonal Carcinoma Cell Pericellular Matrix Embryonal Rhabdomyosarcoma Human Embryonal Carcinoma Cell Cell Line BeWo 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Hassell JR, Kimura JH, Hascall VC (1986) Proteoglycan core protein families. Annu Rev Biochem 55: 539–567PubMedCrossRefGoogle Scholar
  2. Kapoor R, Prehm P (1983) Changes in proteoglycan composition of F9 teratocarcinoma cells upon differentiation. Eur J Biochem 137: 589–595PubMedCrossRefGoogle Scholar
  3. Pera MF, Blasco-Lafita MJ, Cooper S, Mason M, Mills J, Monaghan P (1988) Analysis of cell differentiation lineage in human teratomas using new monoclonal antibodies to cytostructural antigens of embryonal carcinoma cells. Differentiation 39: 139–149PubMedCrossRefGoogle Scholar
  4. Pera MF, Cooper S, Mills J, Parrington M (1989) Isolation and Characterization of a multipotent clone of human embryonal carcinoma cells, Differentiation 42: 10–23PubMedCrossRefGoogle Scholar
  5. Ruoslahti E (1988) Structure and biology of proteoglycans. Annu Rev Cell Biol 4: 229–255PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1991

Authors and Affiliations

  • M. D. Mason
    • 1
  • M. F. Pera
    • 1
  1. 1.Institute of Cancer ResearchRoyal Cancer HospitalSutton, Belmont, SurreyGreat Britain

Personalised recommendations