Skip to main content
SpringerLink
Log in

Anticoagulant Actions of Sulphated Polysaccharides

  • Conference paper
New Trends in Haemostasis

  • 71 Accesses

Abstract

The sulphated polysaccharides to be covered in this review can be divided into two main groups: the glycosaminoglycans, comprising heparin, heparan sulphate and dermatan sulphate, which occur naturally and may contribute towards physiological anticoagulation; artificially sulphated polysaccharides, such as pentosan polysulphate — these are naturally occurring polysaccharides of animal or plant origin which have been artificially sulphated in an attempt to confer heparin like anticoagulant properties.

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

Log in via an institution
Chapter
USD 29.95
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
eBook
USD 84.99
Price excludes VAT (USA)
  • Available as EPUB and PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Albada J, Nieuwenhuis H K, Sixma J J (1989) Treatment of acute venous thromboembolism with low molecular weight heparin (Fragmin). Results of a double-blind randomized study. Circulation 80: 935–940

    Article  PubMed  CAS  Google Scholar 

  • Andersson L-O, Barrowcliffe T W, Holmer E, Johnson E A, Sims G E C (1976) Anticoagulant properties of heparin fractionated by affinity chromotography on matrix-bound antithrombin III and by gel filtration. Thromb Res 9: 575–583

    Article  PubMed  CAS  Google Scholar 

  • Barrowcliffe T W, Gray E, Merton R E, Dawes J, Jennings C A, Hubbard A R, Thomas D P (1986) Anticoagulant activities of pentosan polysulphate (Hémoclar) due to release of hepatic triglyceride lipase ( HTGL ). Thromb Haemostas 56: 202–206

    Google Scholar 

  • Barrowcliffe T W, Havercroft S J, Kemball-Cook G, Lindahl U (1987) The effect of Cat+ phospholipid and factor V on the anti-(factor Xa) activity of heparin and its high-affinity oligosaccharides. Biochem J 243: 31–37

    PubMed  CAS  Google Scholar 

  • Barrowcliffe T W, Johnson E W, Eggleton C A, Kemball-Cook G, Thomas D P (1979) Anticoagulant activities of high and low molecular weight heparin fractions. Br J Haematol 41: 573–583

    Article  PubMed  CAS  Google Scholar 

  • Barrowcliffe T W, Le Shirley Y (1989) The effect of calcium chloride on anti-Xa activity of heparin and its molecular weight fractions. Thromb Haemost 62: 950–954

    PubMed  CAS  Google Scholar 

  • Barrowcliffe T W, Merton R E, Havercroft S J, Thunberg L, Lindahl U, Thomas D P (1984) Low affinity heparin potentiates the action of high affinity heparin oligosaccharides. Thromb Res 34: 125133

    Google Scholar 

  • Beeler D L, Marcum J A, Schiffman S, Rosenberg R D (1986) Interaction of factor XIa and antithrombin in the presence and absence of heparin. Blood 67: 1488–1492

    PubMed  CAS  Google Scholar 

  • Beguin S, Lindhout T and Hemker H C (1988) The mode of action of heparin in plasma. Thromb Haemostas 60: 457–462

    CAS  Google Scholar 

  • Bergqvist D, Ljungner H (1981) A comparative study of Dextran 70 and a sulphated polysaccharide in the prevention of postoperative thromboembolic complications. Br J Surg 68: 9–51

    Article  Google Scholar 

  • Bjork I, Olson S T, Shore J D (1989) Molecular mechanisms of the accelerating effect of heparin on the reactions between antithrombin and clotting proteinases. In: Lane D A, Lindahl U (eds) “Heparin; chemical and biological properties, clinical applications”. London: Edward Arnold: 229–274

    Google Scholar 

  • Buchanan M R, Boneu B, Ofosu F, Hirsh J (1985) The relative importance of thrombin inhibition and factor Xa inhibition to the antithrombotic effects of heparin. Blood 65: 198–201

    PubMed  CAS  Google Scholar 

  • Cadroy Y, Dol F, Caranobe C et al (1988) Standard heparin enhances the antithrombotic activity of dermatan sulfate in the rabbit but CY 216 does not. Thromb Haemostas 59: 295–298

    CAS  Google Scholar 

  • Cadroy Y, Harker L A, Hanson S R (1989) Inhibition of platelet-dependent thrombosis by low molecular weight heparin (CY 222): comparison with standard heparin. J Lab Clin Med 114: 349–357

    PubMed  CAS  Google Scholar 

  • Conrad H Edward (1989) Structure of Heparan Sulfate and Dermatan Sulfate. Ann NY Acad Sci 556: 18–28

    Article  Google Scholar 

  • Fernandez F, Van Ryn J, Ofosu F A, Hirsh J, Buchanan M R (1986) The hemorrhagic and antithrombotic effects of dermatan sulfate. Br J Haematol 64: 309–317

    Article  PubMed  CAS  Google Scholar 

  • Fischer A-M, Barrowcliffe T W, Thomas D P (1982) A comparison of pentosan polysulphate (SP54) and Heparin I: Mechanism of Action on Blood Coagulation. Thromb Haemostas 47: 104–108

    Google Scholar 

  • Fischer A-M, Merton R E, Marsh N A, Williams S, Gaffney P J, Barrowcliffe T W, Thomas D P (1982) A comparison of pentosan polysulphate and heparin II: effects of subcutaneous injection. Thromb Haemostas 47: 109–113

    CAS  Google Scholar 

  • Griffith M J (1982) Kinetics of the heparin-enhanced antithrombin III/thrombin reaction. J Biol Chem 257: 7360–7365

    PubMed  CAS  Google Scholar 

  • Hatton M W C, Berry L R, Regoeczi E (1978) Inhibition of thrombin by antithrombin III in the presence of certain glycosaminoglycans found in the mammalian aorta. Thromb Res 13: 655–670

    Article  PubMed  CAS  Google Scholar 

  • Hemker H C, Béguin S, Pieters J, Lindhout T (1989) The ex Vivo Correlate of the Antithrombotic Action of Heparin. Ann NY Acad Sci 556: 146–157

    Article  PubMed  CAS  Google Scholar 

  • Holmer E, Kurachi K, Soderstrom G (1981) The molecular weight dependence of the rate enhancing effect of heparin on the inhibition of thrombin, factor Xa, factor IXa, factor XIa, factor XIIa and kalikrein by antithrombin. Biochem J 193: 395–400

    PubMed  CAS  Google Scholar 

  • Hubbard A R, Jennings C A, Barrowcliffe T W (1984) Anticoagulant properties in vitro of heparan sulphates. Thromb Res 35: 567–576

    Article  PubMed  CAS  Google Scholar 

  • Johnson E A (1984) Heparan sulphates from porcine inestinal mucosa. Preparation and physiochemical properties. Thromb Res 35: 583–588

    Article  PubMed  CAS  Google Scholar 

  • Johnson E A, Mulloy B (1976) The molecular-weight range of mucosalheparin preparations. Carbohydr Res 51: 119–127

    Article  PubMed  CAS  Google Scholar 

  • Jordan R E, Oosta G M, Gardner W T, Rosenberg R D (1980) The kinetics of hemostatic enzyme-antithrombin interactions in the presence of low molecular weight heparin. J Biol Chem 255: 10081–10090

    PubMed  CAS  Google Scholar 

  • Lane D A, Denton J, Flynn A M, Thunberg L, Lindahl U (1984) Anticoagulant activities of heparin oligosaccharides and their neutralisation by platelet factor 4. Biochem J 218: 725–732

    PubMed  CAS  Google Scholar 

  • Lindahl U, Backstrom G, Hook M, Thunberg L, Fransson L-A, Linker A (1979) Structure of the antithrombin-binding site of heparin. Proc Natl Acad Sci USA 76: 3198–3202

    Article  PubMed  CAS  Google Scholar 

  • Marciniak E (1978) Factor Xa inactivation by antithrombin III: Evidence for biological stabilisation of factor Xa by factor Cphospholipid complex. Br J Haematol 124: 391–400

    Google Scholar 

  • Marcum J A, Rosenberg R D (1984) Anticoagulantly active heparin- like molecules from vascular tissue. Biochemistry 23: 1730

    Article  PubMed  CAS  Google Scholar 

  • McNeely T B, Griffith M J (1985) The anticoagulant mechanism of action of heparin in contact-activated plasma: inhibition of factor X activation. Blood 65: 1226–1231

    PubMed  CAS  Google Scholar 

  • Merton R E, Thomas D P (1987) Experimental studies on the relative efficacy of dermatan sulphate and heparin as antithrombotic agents. Thromb Haemostas 58: 839–842

    CAS  Google Scholar 

  • Nesheim M E (1983) A simple rate law that describes the kinetics of the heparin-catalysed reaction between antithrombin III and thrombin. J Biol Chem 258: 14708–14717

    PubMed  CAS  Google Scholar 

  • Ofosu F A, Blajchman M A, Modi G H, Smith L M, Buchanan M R, Hirsh J (1985) The importance of thrombin inhibition for the expression of the anticoagulant activities of heparin, dermatan sulphate, low molecular weight heparin and pentosan polysulphate. Br J Haematol 60: 695–704

    Article  PubMed  CAS  Google Scholar 

  • Ofosu F A, Modi G J, Hirsh J, Buchanan M R, Blajchman M A (1986) Mechanisms for inhibition of the generation of thrombin activity by sulphated polysacchardies. Ann NY Acad Sci 485: 41–55

    Article  PubMed  CAS  Google Scholar 

  • Ofosu F A, Sie P, Modi G J et al (1987) The inhibition of thrombin-dependent positive feedback reactions is critical to the expression of the anticoagulant effects of heparin. Biochem J 243: 579–588

    PubMed  CAS  Google Scholar 

  • Petitou M (1989) Chemical synthesis of heparin. In: Lane D A, Lindahl U (eds) “Heparin”. London: Edward Arnold

    Google Scholar 

  • Pieters J, Lindhout T (1988) The limited importance of factor Xa inhibition to the anticoagulant property of heparin in thromboplastin-activated plasma. Blood 72: 2048–2052

    PubMed  CAS  Google Scholar 

  • Pixley R A, Schapira M, Colman W (1985) Effect of heparin on the inactivation rate of human activated factor XII by antithrombin III. Blood 66: 198–203

    PubMed  CAS  Google Scholar 

  • Pomerantz M W, Owen W G (1978) A catalytic role for heparin. Evidence for a ternary complex of heparin co-factor, thrombin and heparin. Biochim Biophys Acta 535: 66–77

    Google Scholar 

  • Rosenberg R D (1985) Role of heparin and heparin-like molecules in thrombosis and atherosclerosis. Federation Proceedings 44: 404–409

    PubMed  CAS  Google Scholar 

  • Scott C F, Schapira M, Colman R A (1985) Effect of heparin on the inactivation rate of human factor XIa by antithrombin III. Blood 60: 940–947

    Google Scholar 

  • Scully M F, Kakkar V V (1984) Identification of heparin cofactor II as the principal plasma cofactor for the antithrombin activity of pentosan polysulphate (SP54). Thromb Res 36: 187–194

    Article  PubMed  CAS  Google Scholar 

  • Teien A N, Abildgaard U, Hook M (1976) The anticoagulant effect of heparan sulfate and dermatan sulfate. Thromb Res 8: 859–862

    Article  PubMed  CAS  Google Scholar 

  • Tew C J, Lane D A, Thompson E, Ireland H, Curtis J R (1988) Relationship between ex vivo anti-proteinase (factor Xa and thrombin) assays and in vivo anticoagulant effect of a very low molecular weight heparin, CY222. Br J Haematol 70: 335–340

    Article  PubMed  CAS  Google Scholar 

  • Thomas D P, Gray E, Merton R E (1990) Potentiation of the antithrombotic action of dermatan sulphate by small amounts of heparin. Thromb Haemostas: in press

    Google Scholar 

  • Thomas D P, Johnson E A, Barrowcliffe T W (1981) The antithrombotic action of heparan sulphate. Tesi M, Dormandy J (eds) Serono Symposium No 37 “Vascular Occlusion Epidemiological, Pathophysiological and Therapeutic Aspects” London: Academic Press: 377–382

    Google Scholar 

  • Thomas D P, Merton R E, Barrowcliffe T W, Mulloy B, Johnson E A (1979) Anti-Factor Xa activity of heparan sulphate. Thromb Res 14: 301–306

    Article  Google Scholar 

  • Tollefsen Douglas M, Maimone Margaret M, McGuire Elizabeth A, Peacock Monita E (1989) Heparin Cofactor II Activation by Dermatan Sulfate. Ann NY Acad Sci 556: 116–122

    Article  PubMed  CAS  Google Scholar 

  • Tollefsen D M, Petska C A, Monafo M J (1983) Activation of heparin cofactor II by dermatan sulfate. J Biol Chem 258: 6713–6717

    PubMed  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

  1. National Institute for Biological Standards and Control, Blanche Lane, South Mimms, Potters Bar, EN6 3QG, Hertfordshire, UK

    T. W. Barrowcliffe, R. E. Merton, J. Watton, E. Gray & D. P. Thomas

Authors
  1. T. W. Barrowcliffe
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. R. E. Merton
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. J. Watton
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. E. Gray
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. D. P. Thomas
    View author publications

    You can also search for this author in PubMed Google Scholar

Editor information

Editors and Affiliations

  1. I. Medizinische Klinik, Fakultät Klinische Medizin Mannheim, Ruprecht-Karls-Universität Heidelberg, Theodor-Kutzer-Ufer, 6800, Mannheim, Germany

    Job Harenberg , Dieter L. Heene  & Gerd Stehle ,  & 

  2. Karlstraße 4, 6900, Heidelberg, Germany

    Gotthard Schettler (Leiter, Präsident der Heidelberger Akademie der Wissenschaften (bis 30.9 1990) (Leiter, Präsident der Heidelberger Akademie der Wissenschaften (bis 30.9 1990)

Rights and permissions

Reprints and permissions

Copyright information

© 1990 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Barrowcliffe, T.W., Merton, R.E., Watton, J., Gray, E., Thomas, D.P. (1990). Anticoagulant Actions of Sulphated Polysaccharides. In: Harenberg, J., Heene, D.L., Stehle, G., Schettler, G. (eds) New Trends in Haemostasis. Veröffentlichungen aus der Geomedizinischen Forschungsstelle der Heidelberger Akademie der Wissenschaften, vol 1990 / 1990/3. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84318-1_15

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-84318-1_15

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-540-53275-0

  • Online ISBN: 978-3-642-84318-1

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics

Search

Navigation