Abstract
During the last two decades, the characterization of neuropeptides and of their cellular receptors has constituted one of the major goals in neuroscience research and has given rise to the development of new technologies, especially in neurocytochemistry. We will briefly present and discuss here two immunological methodologies leading to the production of antibodies against receptors for neuropeptides: the anti-idiotypic and “anti-complementary peptide” methodologies.
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References
Blalock JE, Whitaker JN, Benveniste EN, Bost KL (1989) Use of peptides encoded by complementary RNA for generating anti-idiotypic antibodies of predefined specificity. In Antibodies, antigens and molecular mimicry. Methods in Enzymology 178: 49–63 Langone J (ed) Academic Press San Diego New York
Bost KL, Smith LR, Blalock JE (1987) A molecular recognition code: its use for purification of ACTH, endorphin and LHRH receptors. In Anti-idiotypes, receptors and molecular mimicry 35–44 Linthicum S, Farid N (eds) Springer Verlag New York Berl in Heidelberg
Cleveland WL, Wasserman NH, Sarangarajan R, Penn AS, Erlanger BF (1983) Monoclonal antibodies to the acetylcholine receptor by a normally functioning auto- anti-idiotypic mechanism. Nature 305: 56–57
Conrath M, Couraud JY, Pradelles PP (1988a) Anti-idiotypic antibodies as a tool for cytochemical identification of substance P receptors in the central nervous system. J Histochem Cytochem 36: 1397–1401
Conrath M, Couraud JY, Qipo A (1988b) Immunocytochemical study of delta opioid and substance P receptors in the central nervous system using anti-idiotypic antibodies (abstract). Int. Congress on Histochemical Techniques, Varna (Sept 1988 )
Couraud JY, Esher E, Regoli D, Imhoff V, Rossignol B, Pradelles P (1985) Anti- substance P anti-idiotypic antibodies: characterization and biological activities. J Biol Chem 260: 9461–9469
Couraud JY, Frobert Y, Conrath M, Renzi D, Grassi J, Drapeau G, Regoli D, Pradelles P (1987) Monoclonal antibodies to substance P: production, characterization of their fine specificity and use in immunocytochemistry. J Neurochem 49: 1708–1719
Couraud JY, Maillet S, Grassi J, Frobert Y, Pradelles P (1989) Characterization of antisubstance P anti-idiotypic antibodies. In Antibodies, antigens and molecular mimicry. Methods in Enzymology 178: 275–300 Langone J (ed) Academic Press San Diego New York
Couraud JY, Maillet S, Conrath M, Calvino B, Pradelles P (1990) Use of anti-idiotypic antibodies as probes for in vitro and in vivo identification of substance Preceptors. Mol Chem Neuropathology, in press
Jerne NK (1974) Towards a network theory of the immune system. Ann Immunol 125C: 373–389
Jessell TM, Womack MD (1985) Substance P and the novel mammalian tachykinins: a diversity of receptors and cellular actions. Trends Neurosci 8: 43–45.
Knigge KM, Piekut DT, Abood LG, Joseph SA, Michael GJ, Xin L, Berlove DJ (1989) Immunocytochemistry of receptors using anti-idiotypic antibodies. In Antibodies, antigens and molecular mimicry. Methods in Enzymology 178: 212–221 Langone J (ed) Academic Press San Diego New York.
Köhler H, Kaveri S, Kieber-Emmons T, Morrow WJW, Muller S, Raychaudhuru S (1989) Idiotypic networks and nature of molecular mimicry: an overview. In Antibodies, antigens and molecular mimicry. Methods in Enzymology 178: 3–35 Langone J (ed) Academic Press San Diego New York.
Kummer W, Fischer A, Preissler U, Couraud JY, Heim C (1990a) Immunohistochemistry of the guinea-pig trachea using an anti-idiotypic antibody recognizing substance P receptors. Histochemistry 93: 541–546.
Kummer W, Fischer A, Couraud JY, Heim C (1990b) Immunohistochemistry of peptides (substance P and VIP) and peptide receptors in the human trachea (abstract). Autonomic Nervous System Symposium, Oxford (Sept. 1990 ).
Liposits Z, Setalo G, Flerko B (1984) Application of the silver gold intensified 3-3′ diaminobenzidine chromogen to the light and electron microscopic detection of the luteinizing hormone-releasing hormone system in the rat brain. Neuroscience 13: 513–520
Maillet S, Imhoff V, Rossignol B, Grassi J, Pradelles P, Couraud JY (1990) Anti- substance P anti-idiotypic antibodies modulate the secretory process in the rat parotid gland in vitro. Eur J Pharmacol, in press.
Nawa H, Hirose T, Takashima H, Inayama S, Nakanishi S (1983) Nucleotide sequences of cloned cDNAs for two types of bovine brain substance P precursor. Nature 306: 32–36
Pascual DW, Blalock JE, Bost KL (1989) Antipeptide antibodies that recognize a lymphocyte substance P receptor. J Immunol 143: 3697–3702.
Shigemoto R, Yokota Y, Tsuchida K, Nakanishi S (1990) Cloning and expression of a rat neuromedin K receptor cDNA. J Biol Chem 265: 623–628.
Smith LR, Bost KL, Blalock JE (1987) Generation of idiotypic and anti-idiotypic antibodies by immunization with peptides encoded by complementary RNA: a possible molecular basis for the network theory. J Immunol 138: 7–9.
Sommer EW, Kazimierczak J, Droz B (1985) Neuronal subpopulations in the dorsal root ganglion of the mouse as characterized by combination of ultrastructural and cytochemical features. Brain Res 346: 310–326.
Strosberg AD (1989) Interaction of anti-idiotypic antibodies with membrane receptors: practical considerations. In Antibodies, antigens and molecular mimicry. Methods in Enzymology 178: 179–190 Langone J (ed.) Academic Press San Diego New York.
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Couraud, JY. et al. (1991). Immunocytochemistry of Neuropeptide Receptors using Anti-Idiotypic Antibodies and Antibodies to Peptides Encoded by Complementary RNA. In: Calas, A., Eugène, D. (eds) Neurocytochemical Methods. NATO ASI Series, vol 58. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84298-6_17
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DOI: https://doi.org/10.1007/978-3-642-84298-6_17
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