Endocytosis in MDCK Cells
In polarized cells the plasma membrane is divided into apical and basolateral domains. Membrane is internalized from both these domains and the internalized components can follow several different routes, for example, they can be transported to the opposite domain of the cell (transcytosis), routed to lysosomes or recycled back to the same domain. Despite these continuous processes the distinct protein and lipid composition of the two surface domains must be maintained. In this review I will discuss experiments which led us to formulate a model of the endocytic pathways in the Madin Darby Canine Kidney (MDCK) cell, a model epithelial cell system (reviewed by Simons and Fuller, 1985), and briefly consider transcytosis of endogenous glycoproteins in these cells.
KeywordsSorting Biotin Kelly Transferrin Hydrolase
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