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Endocytosis pp 317–324Cite as

Endocytosis in MDCK Cells

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Part of the book series: NATO ASI Series ((ASIH,volume 62))

Abstract

In polarized cells the plasma membrane is divided into apical and basolateral domains. Membrane is internalized from both these domains and the internalized components can follow several different routes, for example, they can be transported to the opposite domain of the cell (transcytosis), routed to lysosomes or recycled back to the same domain. Despite these continuous processes the distinct protein and lipid composition of the two surface domains must be maintained. In this review I will discuss experiments which led us to formulate a model of the endocytic pathways in the Madin Darby Canine Kidney (MDCK) cell, a model epithelial cell system (reviewed by Simons and Fuller, 1985), and briefly consider transcytosis of endogenous glycoproteins in these cells.

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References

  • Bacallao, R., C. Antony, C. Dotti, E. Karsenti, E.H.K. Stelzer, and K. Simons. 1989. The subcellular organization of Madin-Darby canine kidney cells during the formation of a polarized epithelium. J. Cell Biol. 109: 2817–2832.

    Article  PubMed  CAS  Google Scholar 

  • Baker, D., L. Hicke, M. Rexach, M. Schleyer, and R. Schekman. 1988. Reconstitution of SEC gene product-dependent intercompartmental protein transport. Cell 54: 335–344.

    Article  PubMed  CAS  Google Scholar 

  • Bomsel, M., K. Prydz, R.G. Parton, J. Gruenberg, and K. Simons. 1989. Endocytosis in filter-grown Madin-Darby canine kidney cells. J. Cell Biol. 109: 3243–3258.

    Article  PubMed  CAS  Google Scholar 

  • Bomsel, M., R.G. Parton, S.A. Kuznetsov, T.A. Schroer, and J. Gruenberg. 1990. Microtubule- and motor-dependent fusion in vitro between apical and basolateral endocytic vesicles from MDCK cells. Cell 62:719–731.

    Article  PubMed  CAS  Google Scholar 

  • Bourne, H.R. 1988. Do GTPases direct membrane traffic in secretion? Cell 53: 669–671.

    Article  PubMed  CAS  Google Scholar 

  • Brändli, A. W., R.G. Parton and K. Simons. 1990. Transcytosis in MDCK cells: identification of glycoproteins transported bidirectionally between both plasma membrane domains. J. Cell Biol. In Press.

    Google Scholar 

  • Casanova, J. E., P.P. Breitfeld, and K.E. Mostov. 1990. Phosphorylation is required for efficient transcytosis of the polymeric immunoglobulin receptor. Science 246: 742–745.

    Article  Google Scholar 

  • Chavrier, P., R. G. Parton, H.P. Hauri, K. Simons, and Marino Zerial.1990. Localization of low molecular weight GTP-binding proteins to exocytic and endocytic compartments. Cell 62: 317–329.

    Article  PubMed  CAS  Google Scholar 

  • Fuller, S.D., and K. Simons. 1986. Transferrin receptor polarity and recycling accuracy in “tight” and “leaky” strains of Madin-Darby canine kidney cells. J. Cell Biol. 103: 1767–1779.

    Article  PubMed  CAS  Google Scholar 

  • Goud, B., A. Salminen, N.C. Walworth, and P.J. Novick. 1988. A GTP-binding protein required for secretion rapidly associates with secretory vesicles and the plasma membrane in yeast. Cell 53: 753–768.

    Article  PubMed  CAS  Google Scholar 

  • Griffiths, G., B. Hoflack, K. Simons, I. Mellman and S. Kornfeld. 1988. The mannose-6-phosphate receptor and the biogenesis of lysosomes. Cell 52: 329–341.

    Article  PubMed  CAS  Google Scholar 

  • Griffiths, G., R. Matteoni, R. Back, and B. Hoflack. 1990. Characterization of the cation-independent mannose-6-phosphate receptor-enriched prelysosmal compartment. J. Cell Sci. 95: 441–461.

    PubMed  CAS  Google Scholar 

  • Gruenberg, J., G. Griffiths, K.E. Howell. 1989. Characterization of the early endosome and putative endocytic carrier vesicles in vivo and with an assay of vesicle function in vitro. J. Cell Biol. 108: 1301–1316.

    Article  PubMed  CAS  Google Scholar 

  • Gruenberg, J. and K.E. Howell. 1989. Membrane traffic in endoeytosis: insights from cell-free assays. Ann. Rev. Cell Biol. 5:453–481.

    Article  PubMed  CAS  Google Scholar 

  • Helenius, A., I. Mellman, D. Wall, and A. Hubbard. 1983. Endosomes. Trends Biochem. Sci. 8: 245–250.

    Article  CAS  Google Scholar 

  • Hughson, E. J. and C.R. Hopkins. 1990. Endocytic pathways in polarized Caco-2 cells: identification of an endosomal compartment accessible from both apical and basolateral surfaces. J.Cell Biol. 110: 337–348.

    Article  PubMed  CAS  Google Scholar 

  • Hunziker W. and I. Mellman. 1989. Expression of macrophage- lymphocyte receptors in MDCK cells: polarity and transcytosis differ for isoforms with or without coated pit localization domains. J. Cell Biol. 109: 3291–3302.

    Article  PubMed  CAS  Google Scholar 

  • Kelly, R.B. 1990. Microtubules, membrane traffic and cell organization. Cell 61: 5–7.

    Article  PubMed  CAS  Google Scholar 

  • Kornfeld, S. and I. Mellman. 1989. The biogenesis of lysosomes. Ann. Rev. Cell Biol. 5: 483–526.

    Article  PubMed  CAS  Google Scholar 

  • Melançon, P., B.S. Glick, V. Malhotra, P.J. Weidman, T. Serafini, M.L. Gleason, L. Orci, and J.E. Rothman. 1987. Involvement of GTP-binding “G” proteins in transport through the Golgi stack. Cell 51:1053–1062.

    Article  PubMed  Google Scholar 

  • Mostov K.E. and D.L Deitcher. 1986. Polymeric immunoglobulin receptor expressed in MDCK cells transcytoses I. Cell 46: 613–621.

    Article  PubMed  CAS  Google Scholar 

  • Mostov, K.E. and N.E. Simister. 1985. Transcytosis. Cell 43: 389–390.

    Article  CAS  Google Scholar 

  • Parton, R.G., K. Prydz, M. Bomsel, K. Simons, and G. Griffiths. 1989. Meeting of the apical and basolateral endocytic pathways of the Madin-Darby canine kidney cell in late endosomes. J. Cell Biol. 109: 3259–3272.

    Article  PubMed  CAS  Google Scholar 

  • Pesonen, M., W. Ansorge, and K. Simons. 1984. Transcytosis of the G protein of vesicular stomatitis virus after implantation into the apical plasma membrane of Madin-Darby Canine Kidney cells. I. Involvement of endosomes and lysosomes. J. Cell Biol. 99: 796–802.

    Article  PubMed  CAS  Google Scholar 

  • Salminen, A., and P.J. Novick. 1987. A ras-like protein is required for a post-Golgi event in yeast secretion. Cell 49: 527–538.

    Article  PubMed  CAS  Google Scholar 

  • Schmitt, H.D., P. Wagner, E. Pfaff, and D. Gallwitz. 1986. The ras-related YPT1 gene product in yeast: a GTP-binding protein that might be involved in microtubule organization. Cell 47:401–412.

    Article  PubMed  CAS  Google Scholar 

  • Segev, N., J. Mulholland, and D. Bostein. 1988. The yeast GTP-binding YPT1 protein and a mammalian counterpart are associated with the secretion machinery. Cell 52, 915–924.

    Article  PubMed  CAS  Google Scholar 

  • Simons K. and S.D. Fuller. 1985. Surface polarity in epithelia. Ann. Rev. Cell Biol. 1: 243–288.

    Article  PubMed  CAS  Google Scholar 

  • Swanson, J.A., A. Bushnell, and S.C. Silverstein. 1987. Tubular lysosome morphology and distribution within macrophages depend on the integrity of cytoplasmic microtubules. Proc. Natl. Acad. Sci. USA 84: 1921–1925.

    Article  PubMed  CAS  Google Scholar 

  • Van Deurs, B., S.H. Hansen, O.W. Petersen, E.L. Melby, and K. Sandvig. 1990. Endoeytosis, intracellular transport and transcytosis of the toxic protein ricin by a polarized epithelium. Eur. J. Cell. Biol 51: 96–109

    PubMed  Google Scholar 

  • Von Bonsdorff, C.-H., S.D. Fuller, and K. Simons. 1985. Apical and basolateral endocytosis in Madin-Darby canine kidney (MDCK) cells grown on nitrocellulose filters. EMBO J. 4: 2781–2792.

    Google Scholar 

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Author information

Authors and Affiliations

  1. European Molecular Biology Laboratory, Postfach 10.2209, D-6900, Heidelberg, Germany

    Robert G. Parton

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  1. Robert G. Parton
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Editor information

Editors and Affiliations

  1. Cell Biology Unit, Louvain University Medical School and International Institute of Cellular and Molecular Pathology, UCL 75.41 75, av. Hippocrate, 1200, Brussels, Belgium

    Pierre J. Courtoy

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© 1992 Springer-Verlag Berlin Heidelberg

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Parton, R.G. (1992). Endocytosis in MDCK Cells. In: Courtoy, P.J. (eds) Endocytosis. NATO ASI Series, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84295-5_39

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  • DOI: https://doi.org/10.1007/978-3-642-84295-5_39

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84297-9

  • Online ISBN: 978-3-642-84295-5

  • eBook Packages: Springer Book Archive

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