Plasma Membrane Biogenesis and Vesicle Traffic in Polarized Hepatocytes: What can Perturbations Tell Us?
Plasma membrane (PM) biogenesis in polarized epithelial cells involves the generation of specialized PM domains, each facing a different environment and containing a distinct set of proteins. Many domain specific integral membrane proteins have complex type glycosylation and are cotranslationally inserted into the rough endoplasmic reticulum and pass through the Golgi complex together. Afterwards, they must be sorted to the correct membrane domain. We are studying PM biogenesis in the rat hepatocyte, a polarized epithelial cell with multiple basal (sinusoidal) and apical (bile canalicular) domains.
KeywordsBile Duct Ligation Colchicine Treatment Bile Canaliculus Canalicular Membrane Basolateral Surface
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