Abstract
Plasma membrane (PM) biogenesis in polarized epithelial cells involves the generation of specialized PM domains, each facing a different environment and containing a distinct set of proteins. Many domain specific integral membrane proteins have complex type glycosylation and are cotranslationally inserted into the rough endoplasmic reticulum and pass through the Golgi complex together. Afterwards, they must be sorted to the correct membrane domain. We are studying PM biogenesis in the rat hepatocyte, a polarized epithelial cell with multiple basal (sinusoidal) and apical (bile canalicular) domains.
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References
Ahnen DJ, Santiago NA, Cezard J-P, Gray GM (1982) Intestinal aminooligopeptidase. In vivo synthesis on intracellular membranes of rat jejunum. J Biol Chem 257:12129–12135
Barnwell SG, Coleman R (1983) Abnormal secretion of proteins into bile from colchicine-treated isolated perfused rat livers. Biochem J 216:409–414
Bartles JR, Feracci HM, Stieger B, Hubbard AL (1987) Biogenesis of the rat hepatocyte plasma membrane in vivo: comparison of the pathways taken by apical and basolateral proteins using subcellular fractionation. J Cell Biol 105:1241–1251
Danielsen EM, Cowell GM (1985) Biosynthesis of intestinal microvillar proteins. Evidence for an intracellular sorting taking place in, or shortly after, exit from the Golgi complex. Eur J Biochem 152:493–499
Dunn WA, Connolly TP, Hubbard AL (1986) Receptor-mediated endocytosis of epidermal growth factor by rat hepatocytes: receptor pathway. J Cell Biol 102:24–36
Durand-Schneider A-M, Maurice M, Dumont M, Feldmann G (1987) Effect of colchicine and phalloidin on the distribution of three plasma membranes in rat hepatocytes: comparison with bile duct ligation. Hepatology 7:1239–1248
Fransen JAM, Ginsel LA, Hauri H-P, Sterchi E, Blok J (1985) Immunoelectron microscopical localization of a microvillus membrane disaccharidase in the human small intestinal epithelium with monoclonal antibodies. Eur J Cell Biol 38:6–15
Lisanti MP, LeBivic A, Sargiacomo M, Rodriguez-Boulan E (1989) Steady-state distribution and biogenesis of endogenous Madin-Darby canine kidney glycoproteins: evidence for intracellular sorting and polarized cell surface delivery. J Cell Biol 109:2117–2127
Massey D, Feracci H, Gorvel J-P, Rigal A, Soulie JM, Maroux S (1987) Evidence for the transit of aminopeptidase N through the basolateral membrane before it reaches the brush border of enterocytes. J Membrane Biol 96:19–25
Matlin KS (1986) The sorting of proteins to the plasma membrane in epithelial cells. J Cell Biol 103:2565–2568
Matter K, Brauchbar M, Bucher K, Hauri H-P (1990) Sorting of endogenous plasma membrane proteins occurs from two sites in cultured human intestinal epithelial cells (Caco-2). Cell 60:429–437
Miller LL, Bly CG, Watson ML, Bale WF (1951) The dominant role of the liver in plasma protein synthesis. A direct study of the isolated perfused rat liver with the aid of lysine-e-C14. J Exp Med 94:431–453
Misek DE, Bard E, Rodriguez-Boulan E (1984) Biogenesis of epithelial cell polarity: intracellular sorting and vectorial exocytosis of an apical plasma membrane glycoprotein. Cell 39:537–546
Mullock BM, Jones RS, Peppard J, Hinton RH (1980) Effect of colchicine on the transfer of IgA across hepatocytes into bile in isolated perfused rat livers. FEBS Lett 120:278–282
Parczyk K, Haase W, Kondor-Koch C (1989) Microtubules are involved in the secretion of proteins at the apical cell surface of the polarized epithelial cells. J Biol Chem 264:16837–16846
Paschal BM, Shpetner HS, Vallee RB (1987) MAP 1C is a microtubule-activated ATPase which translocates microtubules in vitro and has dynein-like properties. J Cell Biol 105:1273–1282
Rank J, Wilson ID (1983) Changes in IgA following varying degrees of biliary obstruction in the rat. Hepatology 3:241–247
Reaven EP, Reaven GM (1980) Evidence that microtubules play a permissive role in heaptocyte very low density lipoprotein secretion. J Cell Biol 84:28–39
Simons K, Fuller SD (1985) Cell surface polarity in epithelia. Annu Rev Cell Biol 1:243–288
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© 1992 Springer-Verlag Berlin Heidelberg
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Hubbard, A.L., Barr, V.A. (1992). Plasma Membrane Biogenesis and Vesicle Traffic in Polarized Hepatocytes: What can Perturbations Tell Us?. In: Courtoy, P.J. (eds) Endocytosis. NATO ASI Series, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84295-5_32
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DOI: https://doi.org/10.1007/978-3-642-84295-5_32
Publisher Name: Springer, Berlin, Heidelberg
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