Endocytosis pp 257-265 | Cite as

Plasma Membrane Biogenesis and Vesicle Traffic in Polarized Hepatocytes: What can Perturbations Tell Us?

  • Ann L. Hubbard
  • Valarie A. Barr
Conference paper
Part of the NATO ASI Series book series (volume 62)

Abstract

Plasma membrane (PM) biogenesis in polarized epithelial cells involves the generation of specialized PM domains, each facing a different environment and containing a distinct set of proteins. Many domain specific integral membrane proteins have complex type glycosylation and are cotranslationally inserted into the rough endoplasmic reticulum and pass through the Golgi complex together. Afterwards, they must be sorted to the correct membrane domain. We are studying PM biogenesis in the rat hepatocyte, a polarized epithelial cell with multiple basal (sinusoidal) and apical (bile canalicular) domains.

Keywords

Albumin Fractionation Methionine Sorting Caco 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1992

Authors and Affiliations

  • Ann L. Hubbard
    • 1
  • Valarie A. Barr
    • 1
  1. 1.Department of Cell Biology and AnatomyThe Johns Hopkins University School of MedicineBaltimoreUSA

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