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Analysis of Receptor Dynamics, as Studied with Surface Replicas

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Part of the book series: NATO ASI Series ((ASIH,volume 62))

Abstract

Cells interact in a variety of ways with their environment. One essential type of interaction involves the recognition by cells of a wide variety of polypeptides in the surrounding milieu. At the surface of a cell, these molecules are recognized and bound. This process is receptor-mediated and is the first step in the physiologic signaling and/or internalization of the ligand. Investigations on the complement and distribution of receptors at the cell surface are of great significance for determining how receptors modulate cellular activities. The increased interest in studying cell surface receptors in recent years has sparked the improvement of morphological methods and the development of visual probes for localizing receptor sites in the electron microscope.

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References

  • Gebhardt R, Robenek H (1987) Ligand-dependent redistribution of the IgA receptor on cultured rat hepatocytes and its disturbance by cytochalasin B. J Histochem Cytochem 35:301–309

    Article  PubMed  CAS  Google Scholar 

  • Nicol A, Nermut MV, Doeinck A, Robenek H, Wiegand Ch, Jockusch BM (1987) Labeling of structural elements at the ventral plasma membrane of fibroblasts with the immunogold technique. J Histochem Cytochem 35:499–506

    Article  PubMed  CAS  Google Scholar 

  • Pinto da Silva P, Kan FWK (1984) Label-fracture: A method for high resolution labeling of cell surfaces. J Cell Biol 99:1156–1161

    Article  Google Scholar 

  • Robenek H, Rassat J, Hesz A, Grünwald J (1982) A correlative study on the topographical distribution of the receptors for low density lipoprotein (LDL) conjugated to colloidal gold in cultured human skin fibroblasts employing thin section, freeze-fracture, deep-etching, and surface replication techniques. Eur J Cell Biol 27:242–250

    PubMed  CAS  Google Scholar 

  • Robenek H, Hesz A (1983) Dynamics of low density lipoprotein receptors in the plasma membrane of cultured human skin fibroblasts visualized by colloidal gold in conjunction with surface replicas. Eur J Cell Biol 31:275–282

    PubMed  CAS  Google Scholar 

  • Robenek H, Severs NJ (1984) Double labeling of lipoprotein receptors in fibroblast cell surface replicas. J Ultrastruct Res 87:149–158

    Article  PubMed  CAS  Google Scholar 

  • Robenek H, Schmitz G, Assmann G (1984) Topography and dynamics of receptors for acetylated and malondialdehyde-modified low-density lipoproteins in the plasma membrane of mouse peritoneal macrophages as visualized by colloidal gold in conjunction with surface replicas. J Histochem Cytochem 32:1017–1027

    Article  PubMed  CAS  Google Scholar 

  • Robenek H, Schmitz G (1985) Receptor domains in the plasma membrane of cultured mouse peritoneal macrophages. Eur J Cell Biol 39:77–85

    PubMed  CAS  Google Scholar 

  • Robenek H (1989a) Distribution and mobility of receptors in the plasma membrane. In: Freeze-Fracture studies of Membranes, Hui SW (ed) CRC Press Inc, New York 61–86

    Google Scholar 

  • Robenek H (1989b) Topography and internalization of cell surface receptors as analysed by surface replica and ultrathin sectioning techniques. In: Electron Microscopic Analysis of Cell Dynamics, Plattner H (ed) CRC Press Ine, New York 141–163

    Google Scholar 

  • Robenek H, Harrach B, Severs NJ (1990) Display of low density lipoprotein receptors is clustered, not dispersed, in fibroblast and hepatocyte plasma membranes. Arteriosclerosis, in press

    Google Scholar 

  • Severs NJ, Robenek H (1983) Detection of microdomains in biomembranes. An appraisal of recent developments in freeze-fracture cytochemistry. Biochem Biophys Acta 737:373–408

    Article  PubMed  CAS  Google Scholar 

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© 1992 Springer-Verlag Berlin Heidelberg

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Robenek, H. (1992). Analysis of Receptor Dynamics, as Studied with Surface Replicas. In: Courtoy, P.J. (eds) Endocytosis. NATO ASI Series, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84295-5_2

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  • DOI: https://doi.org/10.1007/978-3-642-84295-5_2

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84297-9

  • Online ISBN: 978-3-642-84295-5

  • eBook Packages: Springer Book Archive

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