Abstract
The passage of asialofetuin (ASF) through the endocytic pathway of rat hepatocytes is well described and involves binding to asialoglycoprotein receptors, internalisation via coated pits, appearance in an early, peripheral endosomal compartment(s) where the ligand becomes dissociated from receptor, appearance in a late, deep-lying endosomal compartment(s) and finally digestion in lysosomes (for references see Branch et al., 1987; Mullock et al., 1988). 125I-ASF movement through the various endosomal compartments in rat hepatocytes has been demonstrated by isopycnic centrifugation on vertical density gradients of Ficoll (1 – 22% w/v) and Nycodenz (0.25M sucrose – 45% Nycodenz), which respectively separate early from late endosomal compartments and endosomes from lysosomes (Branch et al., 1987; Perez et al., 1988).
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© 1992 Springer-Verlag Berlin Heidelberg
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Luzio, J.P., Mullock, B.M. (1992). The Interaction of Late Endosomes with Lysosomes in a Cell-Free System. In: Courtoy, P.J. (eds) Endocytosis. NATO ASI Series, vol 62. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84295-5_15
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DOI: https://doi.org/10.1007/978-3-642-84295-5_15
Publisher Name: Springer, Berlin, Heidelberg
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