Genetic Transformation of Tomato and Prospects for Gene Transfer
the genetic map of tomato is becoming powdered with classical and molecular markers dispersed among all 12 chromosomes, and the necessary genetic stocks (tester-lines, trisomies and so on) are available;
individual chromosomes can be identified in pachytene stage;
wild relatives of the cultivated tomato provide a virtually inexhaustible source of genetic variability and disease-resistance genes;
though tomato does not have a genome as small as that of Arabidopsis (1 C = 0.07 pg) — the E. coli among plant model systems —, it still ranks low with 0.74 pg per haploid genome in comparison to other plants which play a major role in agricultural and nutritional economics, like Zea mays (1 C = 3 pg) and Pisum sativum (1 C = 3.86 pg) (Galbraith et al. 1983); and
plants can be regenerated from several types of explants and for various tomato genotypes it has become possible to isolate protoplasts which can be grown to calli and regenerated into fertile plants.
KeywordsTransgenic Plant Restriction Fragment Length Polymorphism Coat Protein Transposable Element Crown Gall
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