Genetic Transformation of Tomato and Prospects for Gene Transfer

  • J. Hille
  • P. Zabel
  • M. Koornneef
Part of the Monographs on Theoretical and Applied Genetics book series (GENETICS, volume 14)

Abstract

For molecular biologists, the cultivated tomato has a variety of attractive features which make it a prime candidate for use as a model system. Decades of extensive research by geneticists, plant breeders, physiologists and pathologists have provided the framework of information which is essential for molecular biologists. The following items may serve as typical examples of the versatility of tomato:
  1. i)

    the genetic map of tomato is becoming powdered with classical and molecular markers dispersed among all 12 chromosomes, and the necessary genetic stocks (tester-lines, trisomies and so on) are available;

     
  2. ii)

    individual chromosomes can be identified in pachytene stage;

     
  3. iii)

    wild relatives of the cultivated tomato provide a virtually inexhaustible source of genetic variability and disease-resistance genes;

     
  4. iv)

    though tomato does not have a genome as small as that of Arabidopsis (1 C = 0.07 pg) — the E. coli among plant model systems —, it still ranks low with 0.74 pg per haploid genome in comparison to other plants which play a major role in agricultural and nutritional economics, like Zea mays (1 C = 3 pg) and Pisum sativum (1 C = 3.86 pg) (Galbraith et al. 1983); and

     
  5. v)

    plants can be regenerated from several types of explants and for various tomato genotypes it has become possible to isolate protoplasts which can be grown to calli and regenerated into fertile plants.

     

Keywords

Maize Recombination Polyethylene Glycol Bacillus 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • J. Hille
    • 1
  • P. Zabel
    • 2
  • M. Koornneef
    • 3
  1. 1.Department of GeneticsFree UniversityAmsterdamThe Netherlands
  2. 2.Department of Molecular BiologyAgricultural UniversityWageningenThe Netherlands
  3. 3.Department of GeneticsAgricultural UniversityWageningenThe Netherlands

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