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Picosecond Polarization Studies of Protein Relaxation

  • Xiaoliang Xie
  • J. D. Simon
Conference paper
Part of the Springer Series in Chemical Physics book series (CHEMICAL, volume 53)

Abstract

Protein relaxation in myoglobin (Mb) following the photoelimination of CO from carbonmonoxymyoglobin (MbCO) is examined using picosecond time resolved circular dichroism (CD) and magnetic circular dichroism (MCD) spectroscopies. The transient CD signal reveals a relaxation process which requires several hundred picoseconds. Comparison with optical absorption studies on the Q-band and near infrared absorption of Mb and linear dichroism studies of the Soret optical absorptions suggest that the CD dynamics reflect a conformation relaxation of the surrounding protein structure.

Keywords

Circular Dichroism Circular Dichroism Spectrum Probe Pulse Transient Absorption Magnetic Circular Dichroism 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. 1.
    P.M. Bayley, M. Anson: Biopolymers 13, 401 (1974)CrossRefGoogle Scholar
  2. 2.
    F.A. Ferrone, J.J. Hopfield, S.E. Schnatterly: Rev. Sci. Instrum. 45, 1392 (1974)CrossRefGoogle Scholar
  3. 3.
    D.S. Kliger: Rev. Chem. Intermed. 8, 367 (1987)CrossRefGoogle Scholar
  4. 4.
    I. Tabushi, K. Kamamura, T. Nishiya: J. Am. Chem. Soc. 101, 2785 (1979)CrossRefGoogle Scholar
  5. 5.
    S.J. Milder, S.C. Bjorling, I.D. Kuntz, D.S. Kliger: Biophys. J. 53, 659 (1980)CrossRefGoogle Scholar
  6. 6.
    X. Xie, J.D. Simon: Rev. Sci. Instrum. 60, 2614 (1989)CrossRefADSGoogle Scholar
  7. 7.
    X. Xie, J.D. Simon: J. Opt. Soc. Am. B, in pressGoogle Scholar
  8. 8.
    X. Xie, J.D. Simon: Biophys. J., in preparationGoogle Scholar
  9. 9.
    X. Xie, J.D. Simon: J. Phys. Chem., in preparationGoogle Scholar
  10. 10.
    For a recent review see: R.M. Hochstrasser, C.K. Johnson: In Ultrashort Laser Pulses and Applications, ed. by W. Kaiser, Topics Appl. Phys., Vol. 60 ( Springer, Berlin, Heidelberg 1988 )Google Scholar
  11. 11.
    X. Xie, J.D. Simon: Biochemistry, submittedGoogle Scholar
  12. 12.
    W.A. Eaton, J. Hofrichter: Methods Enzymol. 76, 175 (1981)CrossRefGoogle Scholar
  13. 13.
    D. Magde: J. Chem. Phys. 93, 3717 (1971)Google Scholar
  14. 14.
    M.-C. Hsu, R.W. Woody: J. Am. Chem. Soc. 93, 3515 (1971)CrossRefGoogle Scholar
  15. 15.
    M. Sassaroli, D.L. Rousseau: Biochem. 26, 3092 (1987)CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin, Heidelberg 1990

Authors and Affiliations

  • Xiaoliang Xie
    • 1
  • J. D. Simon
    • 1
  1. 1.Department of ChemistryUniversity of California at San DiegoLa JollaUSA

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