Alkaloids pp 215-234 | Cite as

Genetic and Chemical Analysis for Alkaloids in Papaver

  • J. R. Sharma
  • M. M. Gupta
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 15)


In the preface of the monograph The Opium Poppy, the editors Husain and Sharma (1983) have aptly summed up the significance of Papaver, P. somniferum in particular, in the growth of major world civilization. They stated: “The story of the man’s civilization is, by and large, a chronicle of his socioeconomic development based on the exploitation of enormous plant resources which nature has generously bestowed upon the earth. Though food plants were its pivotal base, other plants equally contributed to its grandeur. For instance, the growth of major world civilizations (Egyptian 1955 b.c., Greek 1500-1000 b.c., Mesopotamian 700 b.c.) is closely associated with recognition of the opium poppy as a useful plant of medicinal significance in pre-historic times.”


High Performance Liquid Chromatography High Performance Liquid Chromatography Analysis Reversed Phase High Performance Liquid Chromatography Opium Poppy Phase High Performance Liquid Chromatography 
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  1. Akhila A, Uniyal GC (1983) Quantitative estimation of opium alkaloids. Indian J Pharm Sci 45:236–238Google Scholar
  2. Asahina H, Kawatani T, Ono M, Fujita S (1957) Studies on poppies and opium. Bull Narc 9:20–33Google Scholar
  3. Balatre P, Traisnel M, Delcambre JP (1960) Paper chromatography of opium alkaloids. Bull Soc Pharm Lille: 143–149 (CA 1962, 56:10288a)Google Scholar
  4. Beasley TH, Smith DW, Ziegler HW, Charles RL (1974) Liquid-chromatographic system for separating opium alkaloids. J Assoc Off Anal Chem 57:85–90PubMedGoogle Scholar
  5. Böhm H (1967) Über Papaver bracteatum III. Mitteilung. Charakteristische Veränderung des Alkaloidspektrums während der Pflanzenentwicklung. Planta Med 15:215–220PubMedCrossRefGoogle Scholar
  6. British Pharmacopoeia London (1980) Her Majesty’s Stationary Office England, vol I, p 316Google Scholar
  7. Brochmann-Hanssen E, Furuya T (1964) Opium alkaloids - separation and identification by gas, thin layer and paper chromatography. J Pharm Sci 53:1549–1550PubMedCrossRefGoogle Scholar
  8. Chalmers RA, Wadds GA (1970) Spectrofluorimetric analysis of mixtures of the principal opium alkaloids. Analyst 95:234–241PubMedCrossRefGoogle Scholar
  9. Chelombit’Ko VA, Mikheev AD (1988) Data on chemotaxonomy of the section Macrantha Elkan of the genus Papaver L. Part I. Alkaloids of Papaver bracteatum Lindl. Rastit Resur 24:400–410 (PBA 59:1407, 1989)Google Scholar
  10. Chichiro VE, Suranova AV (1978) Unified method for the determination of morphine in opium preparations. Farmatsiya (Mosc) 27:76–78Google Scholar
  11. Cromwell BT (1955) The alkaloids. In: Paech K, Tracey MV (eds) Modern methods of plant analysis, vol IV, Springer, Berlin Heidelberg Göttingen, pp 448–460Google Scholar
  12. Dogan M, Gevenkiris A, Tarhan O (1986) The analysis of poppy straw concentrate by high-performance liquid chromatography. Fitoterapia 57:257–261Google Scholar
  13. Dumitrashko AI, Babilev FV (1976) Spectrofluorimetric method for determining morphine and codeine in opium poppy pods. Izv Akad Nauk Mold SSR, Ser Biol Khim Nauk 1:83–84Google Scholar
  14. Fairbairn JW (1976) Conference on medicinal plants, Marianske Lazne (CSSR), 1975. Planta Med 29:26PubMedCrossRefGoogle Scholar
  15. Fisher G, Gillard R (1977) GLC determination of opium alkaloids in Papaveretum. J Pharm Sci 66:421–423PubMedCrossRefGoogle Scholar
  16. Furmanec Dmytro (1974) Quantitative gas chromatographic determination of the major alkaloids in gum opium. J Chromatogr 89:76–79PubMedCrossRefGoogle Scholar
  17. Goldblatt P (1974) Biosynthetic studies in Papaver section Oxytona. Ann Mo Bot Gard 61:264–296CrossRefGoogle Scholar
  18. Haruyo A, Masako O (1957) A unified analyses of opium for main alkaloids by paper chromatography. Eisei Shikenjo Hokoku 75:133–139Google Scholar
  19. Hiroshi S (1961) Analysis of opium alkaloids. XI. A colour reaction of codeine and morphine and its application to colorimetric determination. Yakugaku Zasshi 81: 865–869Google Scholar
  20. Hiroshi S, Kozuo S, Takeshi T (1962) Analysis of opium alkaloids and allied compounds. XV. Colorimetric determination of dihydrocodeine by eeric ammonium nitrate and 2,4- dinitrophenylhydrazine. Yakugaku Zasshi 82:1684–1687Google Scholar
  21. Hodges CC, Rapoport H (1982) Morphinan alkaloids in callus cultures of Papaver somniferum: J Nat Prod 45:481–485CrossRefGoogle Scholar
  22. Husain A, Sharma JR (eds) (1983) The opium poppy: medicinal and aromatic plants series 1. Central Institute of Medicinal and Aromatic Plants (CIMAP), Lucknow (India)Google Scholar
  23. Hutin M, Cave A, Faucher JP (1983) Utilization of higher-performance liquid chromatography in the characterization and determination of alkaloid principles of P. somniferum L. J Chromatogr 268:125–130CrossRefGoogle Scholar
  24. Izmailov NA, Mushinskaya SKh, Danel’yants VA (1963) Paper chromatography for controlling the manufacture of alkaloids from the capsules of oleiferous poppy. II. Determination of morphine and codeine. Med Prom SSSR 17:47–50PubMedGoogle Scholar
  25. Izmailov NA, Mushinskaya SKh, Danel’yants VA (1964) Paper chromatography for controlling the manufacture of alkaloids from the capsules of oleiferous poppy. III. Determination of narcotine, thebaine and papaverine. Med Prom SSSR 18:47–51PubMedGoogle Scholar
  26. Khanna KR, Shukla S (1986) HPLC investigation of the inheritance of major opium alkaloids. Planta Med 52:157–158CrossRefGoogle Scholar
  27. Laane MM, Wold JK, Paulsen BS, Haugli T, Nordal A (1988) Cytogenetic studies in “X-7”, a new line of Papaver bracteatum Lindl. with increased thebaine content. Hereditas 108:187–197CrossRefGoogle Scholar
  28. Lal RK (1988) Genetics of economic traits related to primary and secondary metabolic activities in opium poppy (Papaver somniferum L.). PhD Thesis, Kanpur University, KanpurGoogle Scholar
  29. Lal RK, Sharma JR (1991) Genetics of alkaloids in Papaver somniferum. Planta Med 57:271–274PubMedCrossRefGoogle Scholar
  30. Lalezari I, Nasseri P, Asgharian R (1974) Papaver bracteatum population Arya II. J Pharm Sci 63:1331–1332CrossRefGoogle Scholar
  31. Levy A (1985) A shattering resistant mutant of Papaver bracteatum Lindl.: Characterization and inheritance. Euphytica 34:811–815CrossRefGoogle Scholar
  32. Levy A, Milo J (1991) Inheritance of morphological and chemical characters in interspecific hybrids between Papaver bracteatum and P. pseudo-orientale. Theor Appl Genet 81:537–540CrossRefGoogle Scholar
  33. Levy A, Palevitch D, Lavie D (1981) Genetic improvement of Papaver bracteatum: heritability and selection response of thebaine and seed yields. Planta Med 43:71–76PubMedCrossRefGoogle Scholar
  34. Lurie I (1977) Application of reverse phase ion-pair partition chromatography to drugs of forensic interest. J Assoc Off Anal Chem 60:1035–1040PubMedGoogle Scholar
  35. Mary NY, Brochmann-Hanssen E (1963) Determination of the principal alkaloids of opium by thin layer chromatography. Lloydia 26:223–228Google Scholar
  36. Matantseva EF, Gladyshev PP, Goryaev MI, Bektenova G A (1980) Quantitative determination of opium alkaloids by liquid chromatographic methods. Khim Prir Soedin 5:730–731Google Scholar
  37. Milo J, Levy A, Palevitch D, Ladizinsky G (1987) Thebaine content and yield in induced tetraploid and triploid plants of Papaver bracteatum. Euphytica 36:361–367CrossRefGoogle Scholar
  38. Milo J, Levy A, Ladizinsky G, Palevitch D (1988) Phylogenetic and genetic studies in Papaver section Oxytona: cytogenetics, isozyme analysis and chloroplast DNA variation. Theor Appl Genet 75:795–802CrossRefGoogle Scholar
  39. Milo J, Levy A, Palevitch D, Ladizinsky G (1990) Genetic evidence for the conversion of the morphinan alkaloid thebaine to oripavine in interspecific hybrids between Papaver bracteatum and P. orientale. Heredity 64:367–370CrossRefGoogle Scholar
  40. Nieminen E (1971) Gas-chromatographic determination of opium alkaloids in pharmaceutical products. Farm Aikak 80:342–349Google Scholar
  41. Nobuhara Y, Hirano S, Namba K, Hashimoto M (1980) Separation and determination of opium alkaloids by high-performance liquid chromatography. J Chromatogr 190: 251–255CrossRefGoogle Scholar
  42. Nyman U, Bruhn JG (1979) Papaver bracteatum - summary of current knowledge. Planta Med 35:97–117PubMedCrossRefGoogle Scholar
  43. Nyman U, Hall O (1976) Some varieties of Papaver somniferum L. with changed morphine alkaloid. Hereditas 84:69PubMedCrossRefGoogle Scholar
  44. Ono M, Shimamine M, Takahashi K (1977) Gas-chromatographic determination of the major alkaloids in opium. Eisei Shikensho Hokoku 95:4–9Google Scholar
  45. Ono M, Shimamine M, Takahashi K (1978) Gas and high-speed liquid chromatographic determination of the other alkaloids in opiate preparations. Eisei Shikensho Hokoku 96:63–66Google Scholar
  46. Pettitt BC Jr, Damon CE (1982) Analysis of poppy straw concentrate by high-performance liquid chromatography. J Chromatogr 242:189–192CrossRefGoogle Scholar
  47. Prista LVN, Fontes DM, Alves da Silva (1976) Chromatographic separation and determination of morphine in opium extracts. An Fac Farm Univ Fed Pernambuco 15:69–76Google Scholar
  48. Šantavý F (1970) Papaveraceae alkaloids. In: Manske RHF (ed) The alkaloids: chemistry and physiology, vol 12. Academic Press, New York, pp 333–454Google Scholar
  49. Šantavý F (1979) Papaveraceae alkaloids II. In: Manske RHF, Rodrigo R (eds) The alkaloids, vol 17. Academic Press, New York, pp 385–545Google Scholar
  50. Shafiee A, Lalezari I, Nasseri-Nauri P, Asgharian R (1975) Alkaloids of Papaver orientale and Papaver pseudo-orientale. J Pharm Sci 64:1570–1572PubMedCrossRefGoogle Scholar
  51. Sharma JR, Singh OP (1983) Genetics and genetic improvement. In: Husain A, Sharma JR (eds) The opium poppy. CIMAP, Lucknow (India), pp 39–67Google Scholar
  52. Sharma JR, Lal RK, Mishra HO, Sharma S (1988) Heterosis and gene action for important traits in opium poppy (Papaver somniferum L.). Indian J Genet 48:261–266Google Scholar
  53. Srivastava RK, Sharma JR (1987) Estimation of genetic variance and allied parameters through biparental mating in opium poppy (Papaver somnierum L.). Aust J Agric Res 38:1047–1052CrossRefGoogle Scholar
  54. Srivastava VK, Maheshwari ML (1985) Liquid chromatographic determination of major alkaloids in gum opium. J Assoc Off Anal Chem 68:801–803Google Scholar
  55. Srivastava VK, Pareek SK, Maheshwari ML (1989) Yield and alkaloids profile in poppy capsules. Indian J Pharm Sci 51:133–137Google Scholar
  56. Staba EJ, Zito S, Amin M (1982) Alkaloid production from Papaver tissue cultures. J Nat Prod 45:256–262CrossRefGoogle Scholar
  57. Szántay CS, Blasko G, Barczai-Beke M, Dorneyi G, Pechy P (1983) Studies on the synthesis of morphine. VI. Planta Med 48:207–211PubMedCrossRefGoogle Scholar
  58. Thakur RS (1983) Opium: chemistry and uses. In: Husain A, Sharma JR (eds) The opium poppy. CIMAP, Lucknow (India), pp 117–133Google Scholar
  59. Theuns HG, Janssen RHAM, Salemink CA (1987) The alkaloids of the Papaver section Oxytona Bernb. herbs, spices and medicinal plants. Recent Adv Bot Hort Pharmocol 2:57–110Google Scholar
  60. Vaidya PV, Pundlik MD, Meghal SK (1980) Rapid method for extraction and colorimetric estimation of morphine in Indian opium. J Assoc Off Anal Chem 63:685–688PubMedGoogle Scholar
  61. Verma RK, Uniyal GC, Gupta MM (1990) High-performance liquid chromatography of poppy straw. Indian J Pharma Sci 52:276–278Google Scholar
  62. Vincent PG, Engelke BF (1979) High-pressure liquid chromatographic determination of the five major alkaloids in Papaver somniferum L. and thebaine in Papaver bracteatum Lindl. capsular tissue. J Assoc Off Anal Chem 62:310–314PubMedGoogle Scholar
  63. Wu Cy, Wittick JJ (1977) Separation of five major alkaloids in gum opium and quantitation of morphine, codeine and thebaine by isocratic reverse phase high-performance liquid chromatography. Anal Chem 49:359–363CrossRefGoogle Scholar
  64. Ziegler HW, Beasley TH, Smith DW (1975) Simultaneous assay for six alkaloids in opium using high-performance liquid chromatography. J Assoc Off Anal Chem 58:888–897PubMedGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1994

Authors and Affiliations

  • J. R. Sharma
  • M. M. Gupta

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