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Alkaloid Analysis in Flue-Cured Tobacco

  • Chapter
Alkaloids

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 15))

Abstract

Tobacco (Nicotiana tabacum L.) is the most widely grown commercial non-food plant, with world production at 2.4 million ha in 1991 (USDA-FAS 1991). It is a New World crop that has alternately been valued for its medicinal properties and condemned as being a health hazard. Although there are 64 Nicotiana species currently known (Sisson and Severson 1990), all commercial tobacco belongs to Nicotiana tabacum L. Commercial tobaccos are cultivated for their cured leaf and are classified according to their field production and method of leaf curing (Akehurst 1981). Flue-cured tobacco is one of several classes, it occupies predominate hectarages in the world, and is one of the principal classes used in the manufacture of cigarettes.

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References

  • Akehurst BC (1981) Tobacco, 2nd edn. Longman, New York

    Google Scholar 

  • Bush LP (1981) Physiology and biosynthesis of tobacco alkaloids. Rec Adv Tob Sci 7:75–106

    CAS  Google Scholar 

  • Campbell CR, Chaplin JF, Johnson WH (1982a) Cultural factors affecting yield, alkaloids, and sugars of close-grown tobacco. Agron J 74:279–283

    Article  Google Scholar 

  • Campbell JS, Chaplin JF, Boyette DM, Campbell CR, Crawford CB (1982b) Effect of plant spacings, topping heights, nitrogen rates, and varieties of tobacco on nicotine yield and concentration. Tob Sci 26:66–69

    Google Scholar 

  • Castro A, Monji N, Malkus H, Eisenhart W, McKennis H Jr, Bowman ER (1979) Automated radioimmunoassay of nicotine. Clin Chim Acta 95:473–481

    Article  PubMed  CAS  Google Scholar 

  • Chaplin JF, Miner GS (1980) Production factors affecting chemical components of the tobacco leaf. Rec Adv Tob Sci 6:3–63

    CAS  Google Scholar 

  • Court WA (1986) High performance liquid chromatography of tobacco and tobacco smoke components. Rec Adv Tob Sci 12:143–184

    CAS  Google Scholar 

  • Cundiff RH, Markunas PC (1955) Determination of nicotine, nornicotine, and total alkaloids in tobacco. Anal Chem 27:1650–1653

    Article  CAS  Google Scholar 

  • Cundiff RH, Markunas PC (1960) Modification of the extraction procedure for determination of alkaloids in tobacco. J Assoc Off Agric Chem 43:519–524

    CAS  Google Scholar 

  • Cundiff RH, Markunas PC (1964) Abbreviated techniques for determinations of alkaloids in tobacco using the extraction procedure. Tob Sci 8:136–137

    CAS  Google Scholar 

  • Dawson RF (1945) An experimental analysis of alkaloid production in Nicotiana: the origin of nornicotine. Am J Bot 32:416–423

    Article  CAS  Google Scholar 

  • Enzell CR, Wahlberg I, Aasen AJ (1977) Isoprenoids and alkaloids of tobacco. Prog Chem Org Nat Prod 34:1–79

    CAS  Google Scholar 

  • Fejér-Kossey O (1967) The separation of ten tobacco alkaloids by thin-layer chromatography. J Chromatogr 31:592–593

    Article  PubMed  Google Scholar 

  • Glock E, Wright MP (1963) Determination of nornicotine in tobacco and smoke by the 1,3- indanedione spectrophotometric method. Anal Chem 35:246–251

    Article  CAS  Google Scholar 

  • Green CR, Colby DA, Cooper PJ, Heckman RA, Lyerly LA, Thome FA (1980) Advances in analytical methodology of leaf and smoke. Rec Adv Tob Sci 6:123–183

    CAS  Google Scholar 

  • Griffith RB (1957) The rapid determination of total alkaloids by steam distillation. Tob Sci 1:130–137

    CAS  Google Scholar 

  • Griffith RB, Jeffrey RN (1948) Improved steam distillation apparatus. Application to determination of nicotine in green and dry tobacco. Anal Chem 20:307–311

    Article  CAS  Google Scholar 

  • Harvey WR, Stahr HM, Smith WC (1969) Automated determination of reducing sugars and nicotine alkaloids on the same extract of tobacco leaf. Tob Sci 13:13–15

    Google Scholar 

  • Hawks SN Jr, Collins WK (1983) Principles of flue-cured tobacco production. SN Hawks Jr, WK Collins, Raleigh, NC

    Google Scholar 

  • Hodgson E, Smith E, Guthrie FE (1965) Two dimensional thin-layer chromatography of tobacco alkaloids and related compounds. J Chromatogr 20:176–177

    Article  PubMed  CAS  Google Scholar 

  • Jeffrey RN, Eoff WH (1955) Paper chromatographic method for determining alkaloids in tobacco. Anal Chem 27:1903–1904

    Article  CAS  Google Scholar 

  • Jeffrey RN, Tso TC (1955) Qualitative differences in the alkaloid fraction of cured tobaccos. J Agric Food Chem 3:680–682

    Article  CAS  Google Scholar 

  • Lauterbach JH, Walker KM (1986) Recent advances in gas chromatography and their applications in tobacco research. Rec Adv Tob Sci 12:185–236

    CAS  Google Scholar 

  • Long RC, Woltz WG (1977) Environmental factors affecting chemical composition of tobacco. In: McKenzie JL (ed) Recent advances in the chemical composition of tobacco and tobacco smoke. Proc Am Chem Soc Symp, Am Chem Soc, Agricultural and Food Chemistry Division, pp 116–163

    Google Scholar 

  • Mann TJ, Matzinger DF, Wernsman EA (1975) Genetic control of tobacco constituents. Tob Res 1:1–12

    Google Scholar 

  • McClure WF, Williamson RE (1986) Status of near-infrared technology in the tobacco industry. Rec Adv Tob Sci 12:3–53

    CAS  Google Scholar 

  • Mosley JM, Rayburn CH (1957) Nornicotine-type alkaloids as a factor in the taste and composition of cigarette smoke. Abstr. 11th Tobacco Chemists’ Research Conference, New Haven, CT

    Google Scholar 

  • Mulchi CL (1985) Environmental factors affecting the growth, chemistry, and quality of tobacco. Rec Adv Tob Sci 11:3–46

    CAS  Google Scholar 

  • Rosa N (1979) Pyrolysis-gas chromatographic estimation of tobacco alkaloids and neophytadiene. J Chromatogr 171:419–423

    Article  CAS  Google Scholar 

  • Sadler WW, Chesson RR, Schoenbaum AW (1960) Automated procedure for determining the nicotine content of steam distillates. Tob Sci 4:208–212

    CAS  Google Scholar 

  • Saunders JA, Blume DE (1981) Quantification of major tobacco alkaloids by highperformance liquid chromatography. J Chromatogr 205:147–154

    Article  CAS  Google Scholar 

  • Severson RF, McDuffie KL, Arrendale RF, Gwynn GR, Chaplin JF, Johnson AW (1981) Rapid method for the analysis of tobacco nicotine alkaloids. J Chromatogr 211: 111–121

    Article  CAS  Google Scholar 

  • Sisson VA, Severson RF (1990) Alkaloid composition of the Nicotiana species. Beitr Tabakforsch 14:327–339

    CAS  Google Scholar 

  • Snook ME, Chortyk OT (1986) Advances in lipophilic gel chromatography of tobacco and tobacco smoke components. Rec Adv Tob Sci 12:237–297

    CAS  Google Scholar 

  • Stephens RL, Weybrew JA (1959) Isatin: a color reagent for nornicotine. Tob Sci 3:48–51

    CAS  Google Scholar 

  • Tso TC (1990) Production, physiology, and biochemistry of tobacco plant. IDEALS, Beltsville, MD

    Google Scholar 

  • Tso TC, Kasperbauer MJ, Sorokin TP (1970) Effect of photoperiod and end-of-day light quality on alkaloids and phenolic compounds of tobacco. Plant Physiol 45:330–333

    Article  PubMed  CAS  Google Scholar 

  • United States Department of Agriculture, Foreign Agricultural Service (1991) World tobacco situation. Circ Ser FT-12–91, USDA-FAS, Washington, DC, pp 48–49

    Google Scholar 

  • van Bevel CHM (1953) Chemical composition of tobacco leaves as affected by soil moisture conditions. Agron J 45:611–614

    Article  Google Scholar 

  • Weeks WW, Davis DL, Bush LP (1969) Gas chromatographic separation of tobacco alkaloids. J Chromatogr 43:506–509

    Article  PubMed  CAS  Google Scholar 

  • Weybrew JA, Wan Ismail WA, Long RC (1983) The cultural management of flue-cured tobacco quality. Tob Sci 27:56–61

    Google Scholar 

  • Williams S (1984) Official methods of plant analysis, 14th edn. Association of Official Analytical Chemists, Arlington, VA, pp 62–63

    Google Scholar 

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Authors
  1. C. A. Wilkinson
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  2. W. W. Weeks
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Editor information

Editors and Affiliations

  1. Goldberglein 7, D-91056, Erlangen, Germany

    Hans Ferdinand Linskens

  2. Department of Horticulture, Viticulture and Oenology Waite Agricultural Research Institute, University of Adelaide, Glen Osmond, S.A., 5064, Australia

    John F. Jackson

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© 1994 Springer-Verlag Berlin Heidelberg

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Wilkinson, C.A., Weeks, W.W. (1994). Alkaloid Analysis in Flue-Cured Tobacco. In: Linskens, H.F., Jackson, J.F. (eds) Alkaloids. Modern Methods of Plant Analysis, vol 15. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-84226-9_5

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  • DOI: https://doi.org/10.1007/978-3-642-84226-9_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-84228-3

  • Online ISBN: 978-3-642-84226-9

  • eBook Packages: Springer Book Archive

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