The Cre-lox Recombination System
Over the past 10 years considerable progress has been made in elucidating the biochemical events involved in site-specific recombination. Information has been gathered from a number of well-characterized systems, most notably the bacteriophage λ Int system which has long served as the paradigm for site-specific recombination. A review covering the site-specific recombination field has recently been published (Craig 1988). This review focuses on the well-characterized Cre-lox site-specific recombination system of bacteriophage P1.
KeywordsMigration Tyrosine Recombination Carboxyl Oligomer
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- Moitoso de Vargas L, Pargellis CA, Hasan NM, Bushman EW, Landy A (1988) Autonomous DNA binding domains of λ integrase recognize two different sequence families. Cell 54: 923–929Google Scholar
- Pargellis CA, Nunes-Düby SE, Moitoso de Vargas L, Landy A (1988) Suicide recombination substrates yield covalent λ Int-DNA complexes and lead to identification of the active site tyrosine. J Biol Chem 263: 7678–7685Google Scholar
- Parsons RL, Prasad PV, Harshey RM, Jayaram M (1988) Step-arrest mutants of FLP recombinase: Implications for the catalytic mechanism of DNA recombination. Mol Cell Biol 8: 3303–3310Google Scholar
- Sternberg N (1978) Demonstration and analysis of P1 site-specific recombination using λ-P1 hybrid phages constructed in vitro. Cold Spring Harbor Symp Quant Biol 43: 1143–1146Google Scholar
- Walker DH Jr, Walker JT (1975) Genetic studies of coliphage P1. I. Mapping by use of prophage deletions. J Virol 16: 525–534Google Scholar