Apium graveolens L. (Celery): In Vitro Culture and the Production of Flavors

  • H. A. Collin
  • S. Isaac
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 15)

Abstract

Celery (Apium graveolens L.) is an umbellifer and is therefore a close relative of parsley, parsnip, and carrot. Like many damp-loving umbellifers, wild celery has a wide distribution, extending from Sweden to Algeria, Egypt, Ethiopia, and in Asia from the Caucasus to Baluchistan and to the mountains of India (Atal and Kapur 1977). The wild plant is bitter and was probably used for medicinal purposes before gaining popularity as a herb and a vegetable (Small 1948). The curative powers attributed to celery are many and varied. Both the roots and seeds were used medicinally, especially in obstructions of the liver and spleen and in the treatment of fevers, jaundice and diaorrhea, pains in the chest, windy cholic and as a diuretic (Phillips 1827). Celery seed (or its oil) is also apparently a cure for rheumatism, gout, bronchitis and asthma (Karim and Bhatty 1976), flatulence and colic (Bjeldanes and Kim 1978) and is ascribed the properties of being abortifacient, antiseptic (Bjeldanes and Kim 1978), deobstruent, anti-inflammatory, a cardiac tonic, a sedative (Guenther 1950) and finally and in contradiction to the last, a stimulant (Karim and Bhatty 1976). Despite this wealth of medicinal qualities ascribed to celery and its seed, no pharmaceutical value is at present attributed to it, being regarded as one of many folk remedies (Crosby and Anderson 1963). The exceptions to this view are publications by Kohli et al. (1967) and Bjeldanes and Kim (1978) on the sedative activity of celery seed constituents.

Keywords

Gout Polyketides Geranyl Carvone Silber 

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References

  1. Adamson AD (1971) The market for certain herbaceous essential oils. Trop Prod Inst Rep G54:3–7.Google Scholar
  2. Al-Abta S, Collin HA (1978a) Control of embryoid development in tissue cultures of celery. Ann Bot (London) 42:773–782.Google Scholar
  3. Al-Abta S, Collin HA (1978b) Cell differentiation in embryoids and plantlets of celery tissue cultures. New Phytol 80:517–521.CrossRefGoogle Scholar
  4. Al-Abta S, Galpin IJ, Collin HA (1979) Flavour production in tissue cultures of celery. Plant Sci Lett 16:129–134.CrossRefGoogle Scholar
  5. Andrews JC, Viser ET (1951) The oxalic acid content of some common foods. Food Res 16:306–312.Google Scholar
  6. Atal CK, Kapur BM (eds) (1977) Cultivation and utilisation of medicinal and aromatic plants. Leipzig Press, New Delhi, pp 330–334.Google Scholar
  7. Balbaa SI, Hilal SH, Haggae MY (1976) A study of the fixed oils of the fruits of Carum copticum Benth. and Hook, Apium graveolens L. and Petroselinum sativum Hoffm. growing in Egypt. J Pharm Sci 16:383–390.Google Scholar
  8. Barton DHR, De Vries JX (1963) The constitution of sedanolide. J Chem Soc 2:1916–1919.CrossRefGoogle Scholar
  9. Beier RC, Oertli EH (1983) Psoralen and other linear furocoumarins as phytoalexins in celery. Phytochemistry 22:2595–2597.CrossRefGoogle Scholar
  10. Bentley LE (1952) Occurrence of malonic acids in plants. Nature (London) 170:847–848.CrossRefGoogle Scholar
  11. Berlingozzi S (1927) Hydrophthalides. I. Contribution to the study of the relationship between constituent and odour. Gazz Chim Ital 57:264–268.Google Scholar
  12. Birch AJ, Donovan FW (1953) Studies in relation to biosynthesis. I. Some possible routes to derivaties of orcinol and phloroglucinol. Aust J Chem 6:360–368.CrossRefGoogle Scholar
  13. Bjeldanes LF, Kim IS (1978) A sedative activity of celery oil constituents. J Food Sci 43:143–144.CrossRefGoogle Scholar
  14. Brodelius P, Nilsson (1980) Entrapment of plant cells in different matrices. FEBS Lett 122:312–316.CrossRefGoogle Scholar
  15. Brodelius P, Deus B, Mosbach K, Zenk MH (1979) Immobilised plant cells for the production and transformation of natural products. FEBS Lett 103:93–97.PubMedCrossRefGoogle Scholar
  16. Browers MA, Orton TJ (1986) Celery (Apium graveolens L.). In: Bajaj YPS (ed) Biotechnology in agriculture and forestry, vol 2: Crops I. Springer, Berlin Heidelberg New York Tokyo, pp 405–420.Google Scholar
  17. Buttkus HA (1978) Celery leaf juice — evaluation and utilisation of a product from harvest debris. J Agric Food Chem 26:827–830.CrossRefGoogle Scholar
  18. Chen CH (1976) Vegetative propagation of the celery plant by tissue culture. Proc S Dakota Acad Sci 55:44–48.Google Scholar
  19. Ciamician G, Silber P (1897) Über die hochsiedenden Bestandteile des Sellerieöls. Ber Dtsch Chem Ges 30:492–501.CrossRefGoogle Scholar
  20. Collin HA (1987) Determinants of yield of secondary products in plant tissue cultures. In: Callow JA (ed) Advances in botanical research, vol 13. Academic Press, New York London, pp 145–199.Google Scholar
  21. Collin HA (1988) Flavours. In: Constabel F, Vasil IK (eds) Cell cultures and somatic cell genetics of plants, vol 5. Academic Press, New York London, pp 569–585.Google Scholar
  22. Collin HA, Watts M (1981) Flavour production in culture. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 1. Techniques for propagation and breeding. Macmillan, New York, pp 729–747.Google Scholar
  23. Collin HA, Donovan A, Isaac S (1987) Selection for disease resistance using celery tissue cultures. In: Boccon-Giboud J, Benbadis A, Short KC (eds) Cell culture techniques applied to plant production and breeding. Proc IAPTC, Angers, ENITHP, pp 21–33.Google Scholar
  24. Crosby DG, Anderson LJ (1963) The organic constituents of food. II. Celery J Food Sci 28:640–648.CrossRefGoogle Scholar
  25. Donovan A, Isaac S, Collin HA (1989) Dual fungal and plant cell culture. In: Walker J (ed) Methods in molecular biology. Humana, NJ.Google Scholar
  26. Dunstan DI, Short KC, Merrick MA, Collin HA (1982) Origin and early growth of celery embryoids. New Phytol 91:121–128.CrossRefGoogle Scholar
  27. Fehr D (1974) On the essential oil of celery (Apium graveolens L.) Pharmazie 29:349–351.PubMedGoogle Scholar
  28. Fehr D (1979) Study on the aroma substances of celery (Apium graveolens L.), pt 1. Pharmazie 34:658–662.Google Scholar
  29. Floss HG, Guenther H, Hardwiger LA (1969) Biosynthesis of furanocoumarins in diseased celery. Phytochemistry 8:585–588.CrossRefGoogle Scholar
  30. Gold HJ, Wilson CW (1961) Techniques in the isolation of volatile materials from celery and the identification of some compounds with acidic properties. Proc Fla State Hortic Soc 74:291–296.Google Scholar
  31. Gold HJ, Wilson CW (1963a) The volatile flavour substances of celery. J Food Sci 28:484–488.CrossRefGoogle Scholar
  32. Gold HJ, Wilson CW (1963b) Alkylidene phthalides and dihydropthalides from celery. J Org Chem 28:985–987.CrossRefGoogle Scholar
  33. Guenther E (1950) The essential oils, vol 4. D Van Nostrand Reinhold, New York, pp 591–602.Google Scholar
  34. Hall CB (1957) Composition and organoleptic evaluation of portions of celery stalks. Proc Fla State Hortic Soc 70:204–208.Google Scholar
  35. Hall CB (1959) Observations on celery flavours. Proc Fla State Hortic Soc 72:284–285.Google Scholar
  36. Hartman BG, Hillig F (1934) Acid constituents of food products with special reference to citric, malic and tartaric acids. J Assoc Off Agric Chem 17:522–531.Google Scholar
  37. Heinstein PF (1985) Future approaches to the formation of secondary natural products in cell suspension cultures. J Nat Prod 48:1–9.CrossRefGoogle Scholar
  38. Herrmann K (1978) Review on non-essential constituents of vegetables. III. Carrots, celery, parsnips, beets, spinach, lettuce, endives, chicory, rhubarb and artichokes. Z Lebensmittel Unters Forsch 167:262–273.CrossRefGoogle Scholar
  39. Innocenti G, Dall’Acqua F, Caporale G (1976) Investigations of the content of furocoumarins in Apium graveolens and Petroselinum sativum. Planta Med 29:165–170.PubMedCrossRefGoogle Scholar
  40. Karim A, Bhatty MK (1976) Studies on the essential oils of the Pakistani species of the family Umbelliferae. IV. Apium graveolens Linn. (celery, ajmodn) seed oil. Pak J Sci Ind Res 19:243–246.Google Scholar
  41. Kariyone T, Shimizu S (1953) Synthesis of alkylidene phthalides and their odour. J Pharm Soc Jpn 73:336–338.Google Scholar
  42. Kohli RP, Dua PR, Shanker K, Saxena RC (1967) Some central effects of an essential oil of Apium graveolens Linn. Indian J Med Res 55:1099–1102.PubMedGoogle Scholar
  43. Lindsey K, Yeoman MM (1983) The relationship between growth rate, differentiation and alkaloid accumulation in cell cultures. J Exp Bot 34:1055–1065.CrossRefGoogle Scholar
  44. Lindsey K, Yeoman MM, Black GM, Mavituna F (1983) A novel method for the immobilisation and culture of plant cells. FEBS Lett 155:143–149.CrossRefGoogle Scholar
  45. Lund ED (1978) Thin layer and high pressure liquid Chromatographie analysis of celery seed oil. J Assoc Off Anal Chem 61:1083–1088.Google Scholar
  46. Lund ED, Wagner CJ, Bryan WL (1974) Oils recovered from celery packing house waste. Proc Fl State Hortic Soc 86:255–259.Google Scholar
  47. Macleod AJ, Macleod G, Subramanian G (1988) Volatile aroma constituents of celery. Phytochemistry 27:373–375.CrossRefGoogle Scholar
  48. Mitsuhashi H, Nagai U (1963) Studies on the constituents of Umbelliferae plants. VII. Structure of Ligustilide 2. Tetrahedron 19:1277–1283.CrossRefGoogle Scholar
  49. Mitsuhashi H, Nomura M (1966) Studies on the constituents of Umbelliferae plants. XII. Biogenesis of 3-butylphthalide. Chem Pharm Bull 14:777–778.PubMedCrossRefGoogle Scholar
  50. Mitsuhashi H, Nagai U, Muramatsu T, Tashiro H (1960) Studies on the constituents of Umbelliferae plants. II. Isolation of the active principles of Ligustieum root. Chem Pharm Bull 8:243–245.CrossRefGoogle Scholar
  51. Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497.CrossRefGoogle Scholar
  52. Opdyke DHJ (1974) Monographs on fragrance raw materials. Celery seed oil. Food Cosmet Toxicol 12(Suppl):849–850.CrossRefGoogle Scholar
  53. Orton TJ (1984) Celery. In: Sharp WR, Evans DA, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 2: Crop species. Macmillan, New York, pp 243–267.Google Scholar
  54. Pan H (1960) Burning-numbing taste in celery. Proc F1 State Hortic Soc 73:223–227.Google Scholar
  55. Pan H (1961) Bitterness in celery. J Food Sci 26:337–341.CrossRefGoogle Scholar
  56. Phillips H (1827) History of cultivated vegetables. Coburn, London, pp 38–40.Google Scholar
  57. Rappaport L, Fujii DS, Thompson RH (1980) From cells to celery (Apium graveolens): callus formation and plant regeneration in tissue and liquid suspension cultures. Hort Sci 15:416.Google Scholar
  58. Robbins SRJ, Greenhalgh P (1979) The markets for selected herbaceous essential oils. Trop Prod Inst Rep G 120:33–36.Google Scholar
  59. Scheel LD, Perone VB, Larkin RL, Kupel RE (1963) The isolation and characterisation of two phytotoxic furanocoumarins (Psoralens) from diseased celery. Biochemistry 2:1127–1131.PubMedCrossRefGoogle Scholar
  60. Schramm R (1961) A comparative paper chromatography of organic acids in storage roots of some cultivable Umbelliferae plants. Acta Soc Bot Pol 30:285–292.Google Scholar
  61. Simonsen J, Barton DHR (1952) The terpenes, vol 3. Univ Press, Cambridge.Google Scholar
  62. Small J (1948) Celery seed. Food 17:181–183.Google Scholar
  63. Stahl E (1969) Thin layer chromatography. A laboratory handbook, 2nd edn. Allen & Unwin, London.Google Scholar
  64. van Straten S, de Vrijer F, Beauveser JC (eds) (1977) Volatile compounds in food. Cent Inst Nutrit Food Res. TNO Krips Repro BV, Meppel, Neth.Google Scholar
  65. Watts MJ, Collin HA (1985) Growth and nutrient uptake by immobilised tissue culture cells of celery (Apium graveolens). Plant Science 42:67–72.CrossRefGoogle Scholar
  66. Watts M J, Galpin IJ, Collin HA (1984) The effect of growth regulators, light and temperature on flavour production in celery tissue cultures. New Phytol 98:583–591.CrossRefGoogle Scholar
  67. Watts MJ, Galpin IJ, Collin HA (1985) The effect of greening on flavour production in celery tissue cultures. New Phytol 100:45–56.CrossRefGoogle Scholar
  68. Williams L, Collin HA (1976) Embryogenesis and plantlet formation in tissue cultures of celery. Ann Bot (London) 40:325–332.Google Scholar
  69. Wilson CW III (1969) Terpene and sesquiterpene hydrocarbons in the essential oil from fresh celery. J Food Sci 34:521–524.CrossRefGoogle Scholar
  70. Wilson CW III (1970) Relative recovery of carbonyl compounds of celery essential oil. J Food Sci 35:766–768.CrossRefGoogle Scholar
  71. Wilson CW III, Neubert AM, Veldhuis MK, Miller WH (1967) Recovery of volatile flavours from celery. Proc F1 State Hortic Soc 80:292–296.Google Scholar
  72. Wilson CW III, Wagner CJ, Berry RE, Veldhuis MK (1969) Relationship of method for recovering essential oil from fresh celery on the chemical composition and flavour. Proc F1 State Hortic Sci 82:187–191.Google Scholar
  73. Wu CM, Koehler PE, Ayres JC (1972) Isolation and identification of xanthotoxin (8-methoxypsoralen) and bergapten (5-methoxypsoralen) from celery infected with Sclerotina sclerotiorum. Appl Microbiol 23:852–856.PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • H. A. Collin
    • 1
  • S. Isaac
    • 1
  1. 1.Department of Genetics and MicrobiologyUniversity of LiverpoolLiverpoolUK

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