Analysis of Flower and Pollen Volatiles

  • H. E. M. Dobson
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 12)


Flowers have evolved complex olfactory and visual displays to attract animals for pollination. Many pollinator insects show high specificity to flower volatiles (Dodson et al. 1969; Williams and Whitten 1983; Pham-Delegue et al. 1986, 1989; Borg-Karlson 1990), and variations in their chemical composition could lead to reproductive isolation and speciation in plants. Since the pioneer studies of flower fragrance chemistry versus pollinators carried out on orchids (Kullenberg 1961; Dodson et al. 1969; Hills et al. 1972; Kullenberg and Bergström 1976; Bergström 1978), there has been a growing interest in the biological significance and systematic value of flower volatiles in diverse plant groups (e.g., Thien et al. 1975, 1985; Nilsson 1978; Gregg 1983; Williams 1983; Williams and Whitten 1983; Borg-Karlson and Groth 1986; Bergström 1987; Groth et al. 1987; Robacker et al. 1988; Pham-Delegue et al. 1989; Borg-Karlson 1990; Dobson et al. 1990).


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  1. Altenburger R, Matile P (1988) Circadian rhythmicity of fragrance emission in flowers of Hoya carnosa R. Br. Planta 174: 248–252Google Scholar
  2. Altenburger R, Matile P (1990) Further observations on rhythmic emission of fragrance in flowers. Planta 180: 194–197CrossRefGoogle Scholar
  3. Andersen JF (1987) Composition of the floral odor of Cucurbita maxima Duchesne (Cucurbitaceae). J Agric Food Chem 35: 60–62CrossRefGoogle Scholar
  4. Armbruster WS, Keller S, Matsuki M, Clausen TP (1989) Pollination of Dalechampia magnoliifolia ( Euphorbiaceae) by male euglossine bees. Am J Bot 76: 1279–1285CrossRefGoogle Scholar
  5. Barker NP (1985) Evidence of a volatile attractant in Ficus ingens (Moraceae). Bothalia 15: 607–611Google Scholar
  6. Bergström G (1978) Role of volatile chemicals in Ophrys-pollinator interactions. In: Harborne G (ed) Biochemical aspects of plant and animal coevolution. Academic Press, London, pp 207–231Google Scholar
  7. Bergström G (1987) On the role of volatile chemical signals in the evolution and speciation of plants and insects: why do flowers smell and why do they smell differently? In: Labeyrie V, Fabres G, Lachaise D (eds) Insect-plants. W Junk, Dordrecht, pp 321–327Google Scholar
  8. Bergstrom G, Appelgren M, Borg-Karlson A-K, Groth I, Stromberg S, Stromberg S (1980) Studies on natural odoriferous compounds XXII. Techniques for the isolation/enrichment of plant volatiles in the analyses of Ophrys orchids ( Orchidaceae ). Chem Scr 16: 173–180Google Scholar
  9. Bergstrom J, Bergstrom G (1989) Floral scents of Bartsia alpina (Scrophulariaceae): chemical composition and variation between individual plants. Nord J Bot 9: 363–365CrossRefGoogle Scholar
  10. Boland W, Ney P, Jaenicke L, Gassmann G (1984) A “closed-loop-stripping” technique as a versatile tool for metabolic studies of volatiles. In: Schreier P (ed) Analysis of volatiles, methods and applications. de Gruyter, Berlin, pp 371–380Google Scholar
  11. Borg-Karlson A-K (1990) Chemical and ethological studies of pollination in the genus Ophrys (Orchidaceae), review article. Phytochemistry 29: 1359–1387CrossRefGoogle Scholar
  12. Borg-Karlson A-K, Groth I (1986) Volatiles from the flowers of four species in the sections Arachnitiformes and Araneiferae of the genus Ophrys as insect mimetic attractants. Phytochemistry 25: 1297–1299CrossRefGoogle Scholar
  13. Borg-Karlson A-K, Bergstrom G, Groth 1(1985a) Chemical basis for the relationship between Ophrys orchids and their pollinators I. Volatile compounds of Ophrys lutea and O. fusca as mimetic attractants/excitants. Chem Scr 25: 283–294Google Scholar
  14. Borg-Karlson A-K, Eidmann HH, Lindstrom M, Norin T, Wiersma N (1985) Odoriferous compounds from the flowers of the conifers Picea abies, Pinus sylvestris and Larix sibirica. Phytochemistry 24: 455–456CrossRefGoogle Scholar
  15. Borg-Karlson A-K, Bergstrom G, Kullenberg B (1987) Chemical basis for the relationship between Ophrys orchids and their pollinators II. Volatile compounds of O. insectifera and O. speculum as insect mimetic attractants/excitants. Chem Scr 27: 303–311Google Scholar
  16. Burger BV, Munro ZM, Visser JH (1988) Determination of plant volatiles 1: analysis of the insect-attracting allomone of the parasitic plant Hydnora africana using Grob-Habich activated charcoal traps. J High Resolut Chromatogr Chromatogr Commun 11: 496–499CrossRefGoogle Scholar
  17. Buttery RG, Kamm JA, Ling LC (1982) Volatile components of alfalfa flowers and pods. J Agric Food Chem 30: 739–742CrossRefGoogle Scholar
  18. Buttery RG, Kamm JA, Ling LC (1984) Volatile components of red clover leaves, flowers, and seed pods: possible insect attractants. J Agric Food Chem 32: 254–256CrossRefGoogle Scholar
  19. Buttery RG, Maddox DM, Light DM, Ling LC (1986) Volatile components of yellow starthistle. J Agric Food Chem 34: 786–788CrossRefGoogle Scholar
  20. Cernaj P, Repcâk M, Tesafik K, Honcariv R (1983) Terpenoid compounds from different parts of Achillea collina Becker inflorescences. Biol Plant 25: 221–224CrossRefGoogle Scholar
  21. Chang JF, Benedict JH, Payne TL, Camp BJ (1988) Volatile monoterpenes collected from the air surrounding flower buds of seven cotton genotypes. Crop Sci 28: 685–688CrossRefGoogle Scholar
  22. Cole RA (1980) The use of porous polymers for the collection of plant volatiles. J Sci Food Agric 31: 1242–1249CrossRefGoogle Scholar
  23. Crisp S (1980) Solid sorbent gas samplers. Ann Occup Hyg 23: 47–76PubMedCrossRefGoogle Scholar
  24. Dobson HEM (1987) Role of flower and pollen aromas in host-plant recognition by solitary bees. Oecologia 72: 618–623CrossRefGoogle Scholar
  25. Dobson HEM (1988) Survey of pollen and pollenkitt lipidschemical cues to flower visitors? Am J Bot 75: 170–182CrossRefGoogle Scholar
  26. Dobson HEM, Bergstrom J, Bergstrom G, Groth I (1987) Pollen and flower volatiles in two Rosa species. Phytochemistry 26: 3171–3173CrossRefGoogle Scholar
  27. Dobson HEM, Bergstrom G, Groth I (1990) Differences in fragrance chemistry between flower parts of Rosa rugosa ( Rosaceae ). Isr J Bot 39: 143–156Google Scholar
  28. Dodson CH, Dressler RL, Hills HG, Adams RM, Williams NH (1969) Biologically active compounds in orchid fragrances. Science 164: 1243–1249PubMedCrossRefGoogle Scholar
  29. Erickson BJ, Young AM, Strand MA, Erickson EH (1987) Pollination biology of Theobroma and Herrania (Sterculiaceae) II. Analyses of floral oils. Insect Sci Appl 8: 301–310Google Scholar
  30. Etievant PX, Azar M, Pham-Delegue MH, Masson CJ (1984) Isolation and identification of volatile constituents of sunflowers (Helianthus annuus L.). J Agric Food Chem 32: 503–509CrossRefGoogle Scholar
  31. Flath RA, Mon TR, Lorenz G, Whitten CJ, Mackley JW (1983) Volatile components of Acacia sp. blossoms. J Agric Food Chem 31: 1167–1170CrossRefGoogle Scholar
  32. Gregg KB (1983) Variation in floral fragrances and morphology: incipient spéciation in Cycnoches? Bot Gaz 144: 566–576Google Scholar
  33. Groth I, Bergström G, Pellmyr O (1987) Floral fragrances in Cimicifuga: chemical polymorphism and incipient speciation in Cimicifuga simplex. Biochem Syst Ecol 15: 441–444CrossRefGoogle Scholar
  34. Hamilton-Kemp TR, Rodriguez JG, Archbold DD, Andersen RA, Loughrin JH, Patterson CG, Lowry SR (1989) Strawberry resistance to Tetranychus urticae Koch: effects of flower, fruit, and foliage removal—comparisons of air-vs. nitrogen-entrained volatile compounds. J Chem Ecol 15: 1465–1473CrossRefGoogle Scholar
  35. Harada K, Mihara S (1984) The volatile constituents of Freesia flower (Freesia hybrida Hort.) Agric Biol Chem 48: 2843–2845Google Scholar
  36. Hibbard BE, Bjostad LB (1988) Behavioral responses of western corn rootworm larvae to volatile semiochemicals from corn seedlings. J Chem Ecol 14: 1523–1539CrossRefGoogle Scholar
  37. Hills HG, Williams NH, Dodson CH (1972) Floral fragrances and isolating mechanisms in the genus Catasetum ( Orchidaceae ). Biotropica 4: 61–76CrossRefGoogle Scholar
  38. Holman RT, Heimermann WH (1973) Identification of components of orchid fragrances by gas chromatography-mass spectrometry. Am Orchid Soc Bull 42: 678–682Google Scholar
  39. Joulain D (1986) Study of the fragrance given off by certain springtime flowers. In: Brunkel E-J (ed) Progress in essential oil research. Walter de Gruyter, Berlin, pp 57–67Google Scholar
  40. Joulain D (1987) The composition of the headspace from fragrant flowers: further results. Flay Fragr J 2: 149–155CrossRefGoogle Scholar
  41. Kaiser R, Lamparsky D (1982) Constituants azotés en trace de quelques absolues de fleurs et leurs headspaces correspondants. In: VIIIe Congrès Intern Huiles Essen 1980 Annales Techn, Fedarom Ed, Grasse, pp 287–294Google Scholar
  42. Krumperman PH (1972) Erroneous peaks from Porapak-Q traps. J Agric Food Chem 20: 909CrossRefGoogle Scholar
  43. Kullenberg B (1961) Studies in Ophrys pollination. Zool Bidr Upps 34: 1–340Google Scholar
  44. Kullenberg B, Bergström G (1976) Hymenoptera aculeata males as pollinators of Ophrys orchids. Zool Scr 5: 13–23CrossRefGoogle Scholar
  45. Kumar N, Motto MG (1986) Volatile constituents of Peony flowers. Phytochemistry 25: 250–253CrossRefGoogle Scholar
  46. Lamparsky D (1985) Headspace techniques as a versatile complementary tool to increase knowledge about constituents of domestic or exotic flowers and fruits. In: Svendsen AB, Scheffer JJC (eds) Essential oils and aromatic plants. W Junk, Dordrecht, pp 79–92CrossRefGoogle Scholar
  47. Lewis JA, Moore CJ, Fletcher MT, Drew RA, Kitching W (1988) Volatile compounds from the flowers of Spathiphyllum cannaefolium. Phytochemistry 27: 2755–2757CrossRefGoogle Scholar
  48. Lindeman A, Jounela-Eriksson P, Lounasmaa M (1982) The aroma composition of the flower of meadowsweet (Filipendula ulmaria (L.)Maxim.) Lebensm Wiss Technol 15: 286–289Google Scholar
  49. Loper GM (1976) Differences in alfalfa flower volatiles among parent and Fl plants. Crop Sci 16: 107–110CrossRefGoogle Scholar
  50. Loper GM, Berdel RL (1978) Seasonal emanation of ocimenc from alfalfa flowers with three irrigation treatments. Crop Sci 18: 447–452CrossRefGoogle Scholar
  51. Loper GM, Lapioli AM (1971) Photoperiodic effects on the emanation of volatiles from alfalfa (Medicago sativa L.) florets. Plant Physiol 49: 729–732CrossRefGoogle Scholar
  52. Lorbeer E, Mayr M, Hausmann B, Kratzl K (1984) Zur Identifizierung flüchtiger Substanzen aus biologischem Material mit Hilfe der CLSA ( Closed Loop Stripping Apparatus ). Monatsh Chem 115: 1107–1112CrossRefGoogle Scholar
  53. Loughrin JH, Hamilton-Kemp TR, Andersen RA, Hildebrand DF (1990) Headspace compounds from flowers of Nicotiana tabacum and related species. J Agric Food Chem 38: 455–460CrossRefGoogle Scholar
  54. Matile P, Altenburger R (1988) Rhythms of fragrance emission in flowers. Planta 174: 242–247CrossRefGoogle Scholar
  55. McCabe T, Hiller JF, Morabito PL (1989) An automated large volume on-column injection technique for capillary gas chromatography. J High Resolut Chromatogr 12: 517–521CrossRefGoogle Scholar
  56. Nilsson LA (1978) Pollination ecology and adaptation in Platanthera chlorantha (Orchidaceae). Bot Not 131: 35–51Google Scholar
  57. Nilsson LA (1981) The pollination ecology of Listera avala (Orchidaceae). Nord J Bot 1: 461–480CrossRefGoogle Scholar
  58. Nilsson LA (1983) Anthecology of Orchis morio (Orchidaceae) at its outpost in the north. Nova Acta Reg Soc Sci Upsaliensis Ser V:C 3: 167–179Google Scholar
  59. Nilsson LA, Jonsson L, Rason L, Randrianjohany E (1985) Monophily and pollination mechanisms in Angraecum arachnites Schltr. (Orchidaceae) in a guild of long-tongued hawk-moths ( Sphingidae) in Madagascar. Biol J Linn Soc 26: 1–19CrossRefGoogle Scholar
  60. Patt JM, Rhoades DF, Corkill JA (1988) Analysis of the floral fragrance of Platanthera stricta. Phytochemistry 27: 91–95CrossRefGoogle Scholar
  61. Pellmyr O, Bergström G, Groth I (1987) Floral fragrances in Actaea, using differential chromatograms to discern between floral and vegetative volatiles. Phytochemistry 26: 1603–1606CrossRefGoogle Scholar
  62. Pham-Delegue MH, Masson C, Etievant P, Azar M (1986) Selective olfactory choices of the honeybee among sunflower aromas: a study of combined olfactory conditioning and chemical analysis. J Chem Ecol 12: 781–793CrossRefGoogle Scholar
  63. Pham-Delegue MH, Etievant P, Guichard E, Masson C (1989) Sunflower volatiles involved in honeybee discrimination among genotypes and flowering stages. J Chem Ecol 15: 329–343CrossRefGoogle Scholar
  64. Porsch O (1956) Windpollen und Blumeninsekt. Österr Bot Z 103: 1–18CrossRefGoogle Scholar
  65. Robacker DC, Meeuse BJD, Erickson EH (1988) Floral aroma: how far will plants go to attract pollinators? BioScience 38: 390–398CrossRefGoogle Scholar
  66. Schaefer J (1981) Comparison of adsorbents in head space sampling. In: Schreier P (ed) Flavour ‘81. Third Weurman Symp Proc Intern Conf., Walter de Gruyter, Berlin, pp 301–313Google Scholar
  67. Takeoka G, Ebeler S, Jennings W (1985) Capillary gas chromatography analysis of volatile flavor compounds. In: Bills DD, Mussinan CI (eds) Characterization and measurement of flavor compounds. Am Chem Soc Symp Ser 289, Washington, DC, pp 95–108CrossRefGoogle Scholar
  68. Tatsuka K, Kohama M, Suekane S (1988) Floral fragrance components of Zygopetalum mackayi ( Orchidaceae ). Agric Biol Chem 52: 1599–1600CrossRefGoogle Scholar
  69. Thien LB, Heimermann WH, Holman RT (1975) Floral odors and quantitative taxonomy of Magnolia and Liriodendron. Taxon 24: 557–568CrossRefGoogle Scholar
  70. Thien LB, Bernhardt P, Gibbs GW, Pellmyr 0, Bergström G, Groth I, McPherson G (1985) The pollination of Zygogynum (Winteraceae) by a moth, Sabatinca (Micropterigidae): an ancient association? Science 227: 540–543PubMedCrossRefGoogle Scholar
  71. Tollsten L, Bergström J (1989) Variation and post-pollination changes in floral odours released by Platanthera bifolia ( Orchidaceae ). Nord J Bot 9: 359–362CrossRefGoogle Scholar
  72. Toulemonde B, Richard HMJ (1983) Volatile constituents of dry elder (Sambucus nigra L.) flowers. J Agric Food Chem 31: 365–370CrossRefGoogle Scholar
  73. von Aufsess A (1960) Geruchliche Nahorientierung der Biene bei entomophilen und ornithophilen Blüten. Z Vergl Physiol 43: 469–498CrossRefGoogle Scholar
  74. von Frisch K (1923) Über die “Sprache” der Bienen. Zool Jahrb Abt Allg Zool Physiol 40: 1–186Google Scholar
  75. von Klimetzek D, Köhler J, Krohn S, Francke W (1989) Das Pheromon-System des WaldrebenBorkenkäfers, Xylocleptes bispinus Duft. (Col., Scolytidae ). J Appl Entomol 107: 304–309CrossRefGoogle Scholar
  76. Wassgren A-B, Bergström G (1984) A simple effluent splitter for capillary gas chromatography. J High Resolut Chromatogr Chromatogr Commun 7: 154–155CrossRefGoogle Scholar
  77. Williams AA, May HV, Tucknott OG (1978) Observations of the use of porous polymers for collecting volatiles from synthetic mixtures reminiscent of fermented ciders. J Sci Food Agric 29: 1041–1054CrossRefGoogle Scholar
  78. Williams NH (1983) Floral fragrances as cues in animal behavior. In: Jones EC, Little RJ (eds) Handbook of experimental pollination biology. Sci Acad Eds Van Nostrand Reinhold, New York, pp 50–72Google Scholar
  79. Williams NH, Whitten M (1983) Orchid floral fragrances and male euglossine bees: methods and advances in the last sesquidecade. Biol Bull 164: 355–395CrossRefGoogle Scholar

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© Springer-Verlag Berlin Heidelberg 1991

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  • H. E. M. Dobson

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