Rice pp 619-641 | Cite as

In Vitro Studies on Rice in Hungary

  • L. E. Heszky
  • Su Nam Li
  • I. K. Simon
  • E. Kiss
  • K. Lökös
  • Quang Binth Do
Part of the Biotechnology in Agriculture and Forestry book series (AGRICULTURE, volume 14)


Hungary, not a typical rice-consuming country, is situated in the northest part of the rice-producing area. The aim of production is self-sufficiency, which requires about 70,000 ha sowing area with a yield of 2.8–3.0 t/ ha (Simon-Kiss 1983).


Salt Tolerance Plant Regeneration Callus Induction Shoot Apex Somaclonal Variation 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Bajaj YPS, Bidani M (1980) Differentiation of genetically variable plants from embryo-derived callus cultures of rice (Oryza sativa L). Phytomorphology 30: 290–299Google Scholar
  2. Bogges S, Stewart CR (1980) The relationship between water stress induced proline accumulation and inhibition of protein synthesis in tobacco leaves. Plant Sci Lett 17: 245–252CrossRefGoogle Scholar
  3. Chon KT, Ge KL, Tsai IS, Yang CS, Tang AW (1983) Callus induction and redifferentiation of different hybrid rice plant parts. In: Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, pp 207–213 Google Scholar
  4. Chu CC (1978) The N6 medium and its applications to anther culture ofcerealcrops.In: Proc Symp Plant tissue culture. Science Press, Beijing, pp 43–50Google Scholar
  5. Greenway H, Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Annu Rev Plant Physiol 31: 149–190CrossRefGoogle Scholar
  6. Harms CT, Oertli JJ (1985) The use of osmotically adapted cell cultures to study salt tolerance in vitro. J Plant Physiol 120: 29–38Google Scholar
  7. Henke RR, Mansut MA, Constantin MJ (1978) Organogenesis and plantlet formation from organ and seedling-derived calli of rice (Oryza saliva). Physiol Plant 44: 11–14CrossRefGoogle Scholar
  8. Heszky LE, Pauk J (1975) Induction of haploid rice plants of different origin in anther culture. Riso 24: 197–204Google Scholar
  9. Heszky LE, Li Su Nam (1984) Effect of callus subculture on plant regeneration capacity in rice. In: Novak FJ, Havel L, Dolezel J (eds) Plant tissue and cell culture, application to crop improvement. Czech Acad Sci Press, Prague, pp 123–124Google Scholar
  10. Heszky LE, Li Su Nam, Horvath Zs (1986) Rice tissue culture and application to breeding: Il Factors affecting the plant regeneration during subculture of diploid and haploid callus.Cereal Res Commun 14: 289–296Google Scholar
  11. Heszky LE, Lökös K, Kiss E, Li Su Nam, Percsich K, Simon-Kiss I (1987) Improvement and application of tissue culture methods to rice (Oryza saliva L.). Bull Agric Sci, Gödöllö 1: 5–15Google Scholar
  12. Heszky LE, Simon-Kiss I, Lökös K, Gyulai G, Kiss E, Geczki I (1989) New rice varieties developed by pollen haploid somaclone method. Proc Vth Scientific Symp Socialist Countries on Biotechnology, Balatonszéplak, Hungary vol II, pp 94–95Google Scholar
  13. Heszky LE, Li Su Nam, Simon-Kiss I, Lökös K, Gyulai G, Kiss E (1989) Tissue specific and ploidy dependent somaclonal variation in rice (Oryza saliva L.). Acta Biologica 40: 381–394PubMedGoogle Scholar
  14. Heyser JW, Nabors MW, MacKinnon C, Dykes TA, De Mott KJ, Kautzman DC, Mujeeb-Kazi A (1985) Long-term, high-frequency plant regeneration and the induction of somatic embryogenesis in callus cultures of wheat (Triticum aestivum L.). Z Pflanzenzwcht 94: 218–233Google Scholar
  15. Inoue M, Maeda E (1980) Effect of auxins and cytokinins on occurrence of green regions in rice callus cultures. Jpn J Crop Sci 49: 167–174CrossRefGoogle Scholar
  16. Li SN, Heszky LE (1984) High frequency of callus induction and plant regeneration in cultured explants of rice varieties. In: Novak FJ, Havel L, Dolezel J (eds) Proc Int Symp Plant Tissue and Cell Culture Application to Crop Improvement. Olomouc, Czechoslovakia, Institute of Experimental Botany, Czechoslovak Academy of Sciences, Prague, pp 121–122Google Scholar
  17. Li SN, Heszky LE (1986a) Testing of salt (NaCI) tolerance and regeneration in callus culture (n, 2n) of rice. In: Horn V, Jensen JC, Odenbach W, Schieder O (eds) Genetic manipulation in plant breeding. De Gruyter, Berlin, pp 617–619Google Scholar
  18. Li SN, Heszky LE (1986b) Rice tissue culture and application to breeding. I. Induction of high totipotent haploid and diploid callus from the different genotypes of rice (Oryza sativa L.). Cereal Res Commun 14: 197–203Google Scholar
  19. Li SN, Heszky LE, Simon-Kiss I, Horvath Zs (1986) Production and applicability of doubled haploid somaclones in rice. Oryza 23: 229–234Google Scholar
  20. Li SN, Heszky LE, Kiss E (1987) Selection for salt tolerance in callus cultures of rice (Oryza sativa L.). Bull Univ Agric Sci, Gödöllö 1: 17–22Google Scholar
  21. Meredith CP (1983) On being selective: mutant from cultured cells. Plant Mol Biol Rep 1:105–110 Müller AI, Grafe R (1978) Isolation and characterization of cell line of Nicotiana tabacum lacking nitrate reductase. Mol Gen Genet 161: 67–76Google Scholar
  22. Nabors MW, Heyser JW, Dykes TA, DeMott KJ (1983) Long-duration high-frequency plant regeneration from cereal tissue cultures. Planta 157: 385–391CrossRefGoogle Scholar
  23. Nishimura S, Maeda E (1977) Histological studies of callus induction in rice seed. Jpn J Crop Sci 46: 275–285CrossRefGoogle Scholar
  24. Oono K (1975) Production of haploid plants of rice (Oryza saliva) by anther culture and their use for breeding. Bull Natl Inst Sci Jpn 26: 139–222Google Scholar
  25. Raghava Ram NV, Nabors MW (1984) Cytokinin mediated long-term high-frequency plant regeneration in rice tissue cultures. Z Pflanzenphysiol 113: 315–323Google Scholar
  26. Simon-Kiss I (1983) Rice production. Mezögazdasagi Kiadb, Budapest, 26 pp (in Hungarian) Siriwardana S, Nabors MW (1983) Tryptophan enhancement of somatic embryogenesis in rice. Plant Physiol 73: 142–146CrossRefGoogle Scholar
  27. Vazquez AM, Ruiz ML, Rueda J, Espino FJ (1984) Plant regeneration studies from embryo derived callus of Hordeum vulgare L. In: Novak FJ, Havel L, Dolezel J (eds) Plant tissue and cell culture application to crop improvement. Czech Acad Sci, Prague, pp 115–116Google Scholar
  28. Wu L, Li HW (1971) Induction of callus tissues initiation from different somatic organs of rice plant by various concentrations of 2,4-dichlorophenoxy acetic acid. Cytologia 36: 411–416CrossRefGoogle Scholar
  29. Yeo AR (1983) Salinity resistance. Physiologies and prices. Physiol Plant 58: 214–222CrossRefGoogle Scholar
  30. Yeo AR, Flowers TJ (1984) Mechanism of salinity resistance in rice and their role as physiological criteria in plant breeding. In: Staples RC, Toenniessen GH (eds) Salinity tolerance in plants. Strategies for crop improvement. Willey Interscience, New York, pp 151–170Google Scholar
  31. Yoshida S, Ogawa M, Suenaga K, He Chun Ye (1983) Induction and selection of salt tolerant mutant rice by tissue culture. Recent progress at IRRI. In: Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, pp 237–254 Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1991

Authors and Affiliations

  • L. E. Heszky
    • 1
  • Su Nam Li
    • 2
  • I. K. Simon
    • 3
  • E. Kiss
    • 1
  • K. Lökös
    • 1
  • Quang Binth Do
    • 4
  1. 1.Department of Genetics and Plant BreedingUniversity of Agricultural SciencesGödöllöHungary
  2. 2.Institute of Experimental BiologyAcademy of SciencesPyongyangDPR, Korea
  3. 3.Rice Breeding SectionInstitute for IrrigationSzarvasHungary
  4. 4.Institute of BiologyAcademy of SciencesHanoiVietnam

Personalised recommendations