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Rice pp 368–382Cite as

Somaclonal Variation for Salt Tolerance in Rice

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Part of the book series: Biotechnology in Agriculture and Forestry ((AGRICULTURE,volume 14))

Abstract

Somaclonal variation for plant improvement is among the most important developments in the agricultural application of this technology; rates of mutation in existing genes have been increased and even new genotypes not found in nature have been engineered. Negative selections using agents such as antibiotic drugs, analogs of metabolic compounds, or simply NaC1 are widely used. The selection of salt-tolerant characteristics is relatively simple, especially if compared to the selection of other characteristics such as high seed protein or disease resistance. However, the main problem in the selection process is the low rate of plant regeneration from different forms of cell aggregates derived from cultured explants. This bars the use of large numbers of regenerants for mass selection. Other problems arise because of the lack of information on what mechanisms produce salt tolerance in cells, tissues, and at whole plant levels. There is also the difficulty of conserving other characteristics of the original varieties in plants selected for their tolerance of salt. The ideal occurrence is a single gene mutation for salt tolerance at a specific locus, which does not involve a chromosome rearrangement in a given adapted variety. This can be achieved through selection of variants resulting from soma-clonal material in vitro in the form of protoplast, cell colony, or callus. Even the selection of entire plants in natural conditions becomes possible, or a combination of these uses, followed by conventional breeding processes.

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References

  • Bajaj YPS, Gupta RK (1987) Plants from salt tolerant cell lines of napier grass Pennisetum purpureum. Schum Ind J Exp Biol 25: 58–60

    Google Scholar 

  • Bright SWJ (1985) Selection in vitro. In: Bright SWJ, Jones MGK (eds) Cereal tissue and cell culture. Nijhoff Junk, Dordrecht, pp 231–260

    Chapter  Google Scholar 

  • Buiatti M (1977) DNA amplification and tissue cultures. In: Reinert J, Bajaj YPS (eds) Applied and fundamental aspects of plant cell, tissue, and organ culture. Springer, Berlin Heidelberg New York, pp 358–373

    Google Scholar 

  • Croughan TP, Stavarek SJ, Rains DW (1978) Selection of NaCI tolerant line of cultured alfalfa cells. Crop Sci 18: 959–963

    Article  CAS  Google Scholar 

  • Dix PJ (1979) Cell culture manipulations as a potential breeding tool. In: Lyons JM, Graham D, Raison JK (eds) Low temperature stress in crop plants. Academic Press, New York London, pp 463–472

    Google Scholar 

  • Dix PJ, Pearce RS (1981) Proline accumulation in NaCI-resistant and sensitive cell lines of Nicotiana sylvestris. Z Pflanzenphysiol 102: 243–348

    CAS  Google Scholar 

  • Flick CE (1983) Isolation of mutants from cell culture. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture, vol 1. Techniques for propagation and breeding. Macmillan, New York, pp 393–441

    Google Scholar 

  • Fukui K (1983) Sequential occurrence of mutation in a growing rice callus. Theor Appl Genet 65: 225–230

    Article  Google Scholar 

  • Greenway H, Munns R (1980) Mechanisms of salt tolerance in nonhalophytes. Annu Rev Plant Physiol 31: 149–190

    Article  CAS  Google Scholar 

  • Hanning G, Nabors MW (1989) In vitro tissue culture selection for sodium chloride (NaCI) tolerance in rice and the performance of the regenerants under saline conditions. In: Proc 2nd Int Symp Genetic manipulation in crops. CIMMYT, Aug 29–31, 1988, Mexico, pp 239–248

    Google Scholar 

  • HeyserJW, Nabors MW (1981) Growth, water content and solute accumulation of two tobacco cell lines cultured on sodium chloride, dextran and polyethyleneglycol. Plant Physiol 68: 429–435

    Article  Google Scholar 

  • Ikehashi H, Ponnamperuma FN (1978) Varietal tolerance of rice for adverse soils. In: Soils and rice. IRRI, Los Banos, Philipp, pp 801–823

    Google Scholar 

  • Kishor Kavi PB, Reddy GM (1986) Improvement of rice for tolerance to salt and drought through tissue culture. Oryza 23: 102–108

    CAS  Google Scholar 

  • Liu MC, Yeh HS (1984) Regeneration of NaCI-tolerant sugarcane plants from callus reinitiated from preselected differentiated shoots. Proc Natl Sci Counc B ROC 8: 110–118

    Google Scholar 

  • Maliga P (1980) Isolation characterization and utilization of mutant cell lines in higher plants. Int Rev Cytol Suppl 11A: 225–250

    Google Scholar 

  • Mathur AK, Ganapathy PS, Johri BM (1980) Isolation of sodium chloride-tolerant plantlets of Kickxia ramossissima under in vitro conditions. Z Pflanzenphysiol 99: 287–249

    CAS  Google Scholar 

  • McHughen A, Swartz M (1984) A tissue-culture derived salt-tolerant line of flax (Linum usitatissimum) J Plant Physiol 117: 109–117

    Google Scholar 

  • Moeljopawiro S, Ikehashi H (1981) Inheritance of salt tolerance in rice. Euphytica 30: 291–300

    Article  Google Scholar 

  • Murashige T, Nakano R (1965) Morphogenetic behavior of tobacco tissue cultures and implication of plant senescence Am J Bot 52: 819–827

    Article  Google Scholar 

  • Nabors MW (1982) Plant tissue culture can help plant breeders produce stress tolerant plant. Tissue Cult Crops Newslett 1: 1–2

    Google Scholar 

  • Nabors MW, Gibbs SE, Bernstein CS, Meis ME (1980) NaCl-tolerant tobacco plants from cultured cells. Z Pflanzenphysiol 97:13–17

    CAS  Google Scholar 

  • Oono K, Sakaguchi S (1980) Mutations induced by rice tissue culture in induction of saline resistant mutants. 7th Symp Plant Tissue and Cell Culture (abstract), p 7 (in Japanese) (cf. Yoshida et al. 1983)

    Google Scholar 

  • Oono K, Okuno K, Kawai T (1984) High frequency of somaclonal mutations in callus culture of rice. Gamma Field Symp 23: 71–95

    Google Scholar 

  • Pearson GS (1959) Factors influencing salinity of submerged soils and growth of Caloro rice. Soil Sci 87: 198–206

    Article  Google Scholar 

  • Petolino JF, Collins GB (1984) Cellular approaches to environmental stress resistance. In: Collins GB, Petolino JG (eds) Application of genetic engineering to crop improvement. NijhoffJunk, Dordrecht, pp 341–390

    Chapter  Google Scholar 

  • Ponnamperuma FN (1984) Role of cultivar tolerance in increasing rice production on saline lands. In: Staples RC, Toenniessen GH (eds) Salinity tolerance in plants. John Wiley & Sons, New York, pp 255–272

    Google Scholar 

  • Reddy PJ, Vaidyanath K (1986) In vitro characterization of salt stress effects and the selection of salt tolerant plants in rice (Oryza sativa L.). Theor Appl Genet 71: 757–760

    Article  Google Scholar 

  • Smith MK, McComb JA (1981) Effect of NaC1 on the growth of whole plants and their corresponding callus cultures. Aust J Plant Physiol 8: 267–275

    Article  CAS  Google Scholar 

  • Staples RC, Toenniessen GH (eds) (1984) Salinity tolerance in plants: strategies for crop improvement. John Wiley & Sons, New York, 443 pp

    Google Scholar 

  • Stavarek SJ, Rains DW (1984) Cell culture techniques: Selection and physiological studies of salt tolerance. In: Staples RC, Toenniessen GH (eds) Salinity tolerance in plants, strategies for crop improvement. John Wiley & Sons, New York, pp 321–334

    Google Scholar 

  • Sun Z, Zhao C, Zheng K, Qi X, Fu Y (1983) Somaclonal genetics of rice, Oryza saliva L. Theor Appl Genet 67: 67–73

    Article  Google Scholar 

  • Tal M (1983) Selection for stress tolerance. In: Evans DA, Sharp WR, Ammirato PV, Yamada Y (eds) Handbook of plant cell culture vol 1. Techniques for propagation and breeding. Macmillan, New York, pp 461–448

    Google Scholar 

  • Tal M (1984) Physiological genetics of salt resistance in higher plants: studies on the level of the whole plant and isolated organs tissues and cells. In: Staples RC, Toenniessen GH (eds) Salinity tolerance in plants, strategies for crop improvement. John Wiley & Sons, New York, pp 301–320

    Google Scholar 

  • Tal M, Katz A (1980) Salt tolerance in the wild relatives of the cultivated tomato: the effect of proline on the growth of callus tissue of Lycopersicon esculentum and L. peruvianum under salt and water stress Z Pflanzenphysiol 98: 283–288

    CAS  Google Scholar 

  • Torrey JG (1967) Morphogenesis in relation to chromosomal constitution in long-term plant tissue cultures. Physiol Plant 20: 265–275

    Article  Google Scholar 

  • Tyagi AK, Rashid A, Maheshwari SC (1981) Sodium chloride resistant cell line from haploid Datura innoxia Mill. A resistance trait carried from cell to plantlet and vice versa in vitro. Protoplasma 105: 327–332

    Article  Google Scholar 

  • Vajrabhaya M, Vajrabhaya T (1986) On the selection of salt tolerant rice from tissue culture. In: Japan-Singapore Seminar on Clonal multiplication and transformation of useful plants through cell and tissue culture. Univ Press, Tsukuba, Jpn, Abstr, p 4

    Google Scholar 

  • Vajrabhaya M, Tunvachkul O, Vajrabhaya T (1987) Toward establishing salt-tolerant lines of rice. In: Proc 2nd Annu Conf Int Plant Biotechnol Network, Bangkok, Abstr 57

    Google Scholar 

  • Wenzel G, Schieder O, Przewozny T, Sopory SK, Melchers G (1979) Comparison of single-cell culture derived Solanum tuberosum L. plants and a model for their application in breeding programs. Theor Appl Genet 55: 49–55

    Article  Google Scholar 

  • Wong CK, Woo SC, Ko SW (1986) Production of rice plantlets on NaCl-stressed medium and evaluation of their progenies. Bot Bull Acad Sin 27: 11–23

    Google Scholar 

  • Yamada Y, Ogawa M, Yano S (1983) Tissue culture in sea water increases salt tolerance of rice plants. In: Proc Worksh Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, pp 229–236

    Google Scholar 

  • Yano S, Ogawa M, Yamada Y (1982) Plant formation from selected rice cells resistant to salts. In: Fujiwara A (ed) Plant Tissue Culture 1982. Maruzen, Tokyo, pp 495–496

    Google Scholar 

  • Yeo AR, Flowers TJ (1983) Varietal differences in the toxicity of sodium ions in rice leaves. Physiol Plant 59: 189–195

    Article  CAS  Google Scholar 

  • Yeo AR, Flowers TJ (1984) Mechanisms of salinity resistance in rice and their role as physiological criteria in plant breeding. In: Staples RC, Toenniessen GH (eds) Salinity tolerance in plants, strategies for crop improvement. John Wiley & Sons, New York, pp 151–170

    Google Scholar 

  • Yeoman MM (ed) (1986) Plant cell culture technology. Botanical monographs, vol 23. Blackwell, Oxford, 375 pp

    Google Scholar 

  • Yoshida S (1981) Fundamental of rice crop science. IRRI, Los Banos, Philipp, 269 pp

    Google Scholar 

  • Yoshida S, Ogawa M, Suenaga K, Ye HC (1983) Induction and selection of salt-tolerant mutant rices by tissue culture — recent progress at IRRI. In: Proc Worksh Cell and tissue culture techniques for cereal crop improvement. Science Press, Beijing, pp 237–254

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department of Botany, Faculty of Science, Chulalongkorn University, Bangkok, 10330, Thailand

    Thavorn Vajrabhaya

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  1. Montakan Vajrabhaya
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  2. Thavorn Vajrabhaya
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  1. A-137 New Friends Colony, 110065, New Delhi, India

    Y. P. S. Bajaj

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© 1991 Springer-Verlag Berlin Heidelberg

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Vajrabhaya, M., Vajrabhaya, T. (1991). Somaclonal Variation for Salt Tolerance in Rice. In: Bajaj, Y.P.S. (eds) Rice. Biotechnology in Agriculture and Forestry, vol 14. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83986-3_24

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  • DOI: https://doi.org/10.1007/978-3-642-83986-3_24

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83988-7

  • Online ISBN: 978-3-642-83986-3

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