Free Calcium in Red Blood Cells of Human Hypertensives is Elevated: How Can This be?
Abnormal regulation of calcium transport has been proposed as one mechanism by which increased levels of intracellular free Ca2+ may occur and could be a factor in essential hypertension (Bohr and Webb, 1984). Since vascular smooth muscle cells from human subjects are not readily available for studies of ion transport, circulating blood cells have frequently been used as a model for such measurements in hypertension (Erne et al., 1984a; Lindner et al., 1987; Postnov et al., 1977). While several abnormal Na+ transport pathways have been described in human red blood cells (RBCs) in hypertension (Postnov et al., 1977; Diez et al., 1987), little is known concerning intracellular free Ca2+ in these cells.
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- Bohr DF, Webb RC (1984) Vascular smooth muscle function and its changes in hypertension. Am J Med 77 Suppl 4A: 3–16Google Scholar
- Dedman JR, Potter JD, Jackson RL, Johnson JD, Means AR (1977) Physicochemical properties of rat testis Ca2+-dependent regulator protein of cyclic nucleotide phosphodiesterase. Relationship of Ca2+-binding, conformational changes and phosphodiesterase activity. J Biol Chem 252: 8415–8422PubMedGoogle Scholar
- Eme P, Bürgisser E, Bolli P, Ji B-H, Bühler F (1984b) Free calcium concentration in platelets closely relates to blood pressure in normal and essentially hypertensive subjects. Hypertension 6: 166–169Google Scholar
- Ferreira HG, Lew VL (1977) Passive Ca transport and cytoplasmic buffering in intact red cells. In Membrane Transport in Red Cells. Ellory JC, Lew VL (eds) Academic Press New York, pp. 53–91Google Scholar
- Hinds TR, Lindner A, Vincenzi FF (1989) Estimation of intracellular free calcium in red blood cells using the fluorescent probe FLUO-3. J Cell Biol 107: 75Google Scholar
- Schatzmann HT, Vincenzi FF (1969) Calcium movements across the membrane of human red cells. J Physiol (Lond) 201: 369–385Google Scholar
- Vincenzi FF, Cambareri JJ (1985) Apparent ionophoric effects of red blood cell deformation. In Cellular and Molecular Aspects of Aging: The Red Cell as a Model. Eaton JW, Konzen DK, White JG (eds) Alan R. Liss New York, pp. 213–222Google Scholar
- Incenzi FF (1990) Regulation of the plasma membrane Cat+-pump. In The Red Cell Membrane. Raess BU, Tunnicliff G (eds) Humana Press Clifton, NJ, pp. 123–142Google Scholar
- Vincenzi FF, DiJulio D, Morris CD, McCarron D (1988) Measurements on the activity of the plasma membrane Ca pump ATPase in human hypertension. In Cellular Calcium and Phosphate Transport in Health and Disease. Bronner F, Peterlik M (eds) Alan R. Liss New York, pp. 379–383Google Scholar
- Vincenzi FF, Hinds TR (1980) Calmodulin and plasma membrane calcium transport. In Calcium and Cell Function, Vol. I. Cheung WY (ed) Academic Press New York, pp. 127–165Google Scholar