Skip to main content

The Sensitivity of Plant Protoplasts to Auxin is Likely Modulated by the Number of Receptors at the Plasmalemma

  • Conference paper

Part of the NATO ASI Series book series (ASIH,volume 47)

Abstract

Auxin modifies the electrical transmembrane potential of isolated protoplasts according to an inverted bell-shaped dose-response curve (Ephritikhine et al, 1987; Shen et al, 1988). The sensitivity of plant protoplasts to auxin can be characterized by the hormone concentration inducing the maximal variation of the transmembrane potential difference (ΔEm). On tobacco mesophyll protoplasts, the response to auxin was blocked by antibodies directed against an auxin-binding protein (ABP) from maize coleoptile membranes, or raised to the plasmalemma ATPase from yeast (Barbier-Brygoo et al, 1989a). The auxin-induced ΔEm thus seems to nvolve the occupancy of an auxin receptor at the cell surface which in turns would activate the proton-pump ATPase at the plasmalemma.

Keywords

  • Antigenic Site
  • Plant Protoplast
  • Tobacco Protoplast
  • Auxin Receptor
  • Tobacco Mesophyll Protoplast

These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

This is a preview of subscription content, access via your institution.

Buying options

Chapter
USD   29.95
Price excludes VAT (USA)
  • DOI: 10.1007/978-3-642-83974-0_1
  • Chapter length: 11 pages
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
eBook
USD   119.00
Price excludes VAT (USA)
  • ISBN: 978-3-642-83974-0
  • Instant PDF download
  • Readable on all devices
  • Own it forever
  • Exclusive offer for individuals only
  • Tax calculation will be finalised during checkout
Softcover Book
USD   159.00
Price excludes VAT (USA)

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Barbier-Brygoo H, Ephritikhine G, Klämbt D, Ghislain M, Guern J (1989a) Functional evidence for an auxin receptor at the plasmalemma of tobacco mesophyll protoplasts. Proc Natl Acad Sci USA 86: 891–896

    PubMed  CrossRef  CAS  Google Scholar 

  • Barbier-Brygoo H, Ephritikhine G, Shen WH, Delbarre A, Klämbt D, Guern J, (1989b) Characterization and modulation of the sensitivity of plant protoplasts to auxin. In NATO ASI Series, Transducing Pathways:Activation and Desensitization, Konijn TM ed., Springer Verlag, in press

    Google Scholar 

  • Barbier-Brygoo H, Guern J, Ephritikhine G, Shen WH, Maurel C, Klämbt D (1989c) The sensitivity of plant protoplasts to auxins: modulation of receptors at the plasmalemma. In “Plant Gene Transfer”, UCLA Symposia on Molecular and Cellular Biology, New Series, Vol. 189, eds Lamb C and Beachy R, Liss AR, Inc., New-York, in press

    Google Scholar 

  • Caboche M (1980) Nutritional requirements of protoplastderived, haploid tobacco cells grown at low cell densities in liquid medium. Planta 149: 7–18

    CrossRef  CAS  Google Scholar 

  • Ephritikhine G, Barbier-Brygoo H, Muller JF, Guern J (1987) Auxin effect on the transmembrane potential difference of wild-type and mutant tobacco protoplasts exhibiting a differential sensitivity to auxin. Plant Physiol 83: 801–804

    PubMed  CrossRef  CAS  Google Scholar 

  • Guern J (1987) Regulation from within: the hormone dilemma. Ann Bot 60: 75–102

    CAS  Google Scholar 

  • Inohara N, Shimomura S, Fukui T, Futai M (1989) Auxin-binding protein located in the endoplasmic reticulum of maize shoots: molecular cloning and complete primary structure. Proc Natl Acad Sci USA 86: 3564–3568

    PubMed  CrossRef  CAS  Google Scholar 

  • Löbler M, Klämbt D (1985) Auxin-binding protein from coleoptile membranes of corn (Zea mays L.). I Purification by immunological methods. J Biol Chem 260:9848–9853

    PubMed  Google Scholar 

  • Napier RM, Venis MA, Bolton MA, Richardson LI, Butcher GW (1988) Preparation and characterisation of monoclonal and polyclonal antibodies to maize membrane auxin-binding protein. Planta 176: 519–526

    CrossRef  CAS  Google Scholar 

  • Shen WH, Petit A, Guern J, Témpé J (1988) Hairy roots are more sensitive to auxin than normal roots. Proc Natl Acad Sci USA 85: 3417–3421

    PubMed  CrossRef  CAS  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and Permissions

Copyright information

© 1990 Springer-Verlag Berlin Heidelberg

About this paper

Cite this paper

Barbier-Brygoo, H., Ephritikhine, G., Klämbt, D., Guern, J. (1990). The Sensitivity of Plant Protoplasts to Auxin is Likely Modulated by the Number of Receptors at the Plasmalemma. In: Ranjeva, R., Boudet, A.M. (eds) Signal Perception and Transduction in Higher Plants. NATO ASI Series, vol 47. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83974-0_1

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-83974-0_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83976-4

  • Online ISBN: 978-3-642-83974-0

  • eBook Packages: Springer Book Archive