GTP-Binding Proteins associated with CNS Myelin

  • Peter E. Braun
  • Lise Bernier
Part of the NATO ASI Series book series (volume 43)


The unexpected discovery of cholinergic receptor activity (Larocca et al, 1987) in isolated myelin and the abundant, dynamic metabolism of polyphos-phoinositides of PNS and CNS myelin (Kahn and Morell, 1988; Eichberg et al 1989) is forcing a reevaluation of the role of this membranous organelle in the nervous system. Implicit in these considerations is the probable participation of GTP-binding proteins (G proteins) in the signal transduction apparatus.


Myelin Basic Protein Cholera Toxin Pertussis Toxin Myelin Protein Crude Membrane Fraction 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


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  1. Ashkenazi A, Ramachandran J and Capon DJ (1989) Acetylcholine analog stimulates DNA synthesis in brain-derived cells via specific muscarinic receptor subtypes Nature 340: 146–150PubMedCrossRefGoogle Scholar
  2. Barbacid M (1987) ras genes. Ann Rev Biochem 56:779–828.Google Scholar
  3. Barres BA, Chun LLY and Corey DP (1988) Ion channel expression by white matter glia: type 2 astrocytes and oligodendrocytes. Glia 1: 10–30PubMedCrossRefGoogle Scholar
  4. Bernier L, Horvath E and Braun P (1989) GTP binding proteins in CNS myelin. Trans Amer Soc Neurochem 20: 254Google Scholar
  5. Bhullar R and Haslam RJ (1988) Gn-proteins are distinct from ras p.21 and other known low molecular mass GTP-binding proteins in the platelet. FEBS Letts 237: 168–172CrossRefGoogle Scholar
  6. Bhullar RP and Haslam RJ (1987) Detection of 23–27 kDa GTP-binding proteins in platelets and other cells. Biochem J 245: 617–620PubMedGoogle Scholar
  7. Bourne HR (1988) Do GTPases direct membrane traffic in secretion? Cell 53: 669–671.PubMedCrossRefGoogle Scholar
  8. Burgoyne RD (1987) Control of exocytosis. Nature 328: 112–113PubMedCrossRefGoogle Scholar
  9. Nagata K, Nagao S and Nozawa Y (1989) Low Mr GTP-binding proteins in human platelets: cyclic AMP-dependent protein kinase phosphorylates m22KG(I) in membrane but not c21KG in cytosol. Biochem Biophys Res Comm 160: 235–242.PubMedCrossRefGoogle Scholar
  10. Comerford JG, Gibson JR, Dawson AP, Gibson I (1989) RAS p21 and other Gn proteins ore detected in mammalian cell lines by (y35S)GTP yS binding. Biochem Biophys Res Comm 159: 1269–1274PubMedCrossRefGoogle Scholar
  11. Eichberg J, Berti-Mattera LN, Day S-F, Lowery J and Zhu X (1989) Basal and receptor-stimulated metabolism of polyphosphoinositides in Peripheral nerve myelin. J Neurochem 52:524 AGoogle Scholar
  12. Freissmuth M, Casey PJ and Gilman AG (1989) G proteins control diverse pathways of transmembrane signaling. The FASEB J 3: 2125–2131.Google Scholar
  13. Gilman AG (1987) G proteins: transducers of receptor-generated signals. Ann Rev Biochem 56: 615–650PubMedCrossRefGoogle Scholar
  14. Hancock JF, Magee AI, Childs JE and Marshall CJ (1989) All ras proteins are polyisoprenylated but only some are palmitoylated. Cell 57: 1167–1177PubMedCrossRefGoogle Scholar
  15. Kahn DW and Morell P (1988) Phosphatidic acid and phosphoinositide turnover in myelin and its stimulation by acetylcholine. J Neurochem 50: 1542–1550PubMedCrossRefGoogle Scholar
  16. Kamata, T. and Kung, H.F. (1988) Effects of ras-encoded proteins and platelet-derived growth factor on inositol phospholipid turnover in NRK cells. Proc Natl Acad Sci (USA) 85: 5799–5803CrossRefGoogle Scholar
  17. Kikuchi A, Yamamoto K, Fujita T and Takai Y (1988) ADP-ribosylation of the bovine brain rho protein by botulinum toxin type Cl. J Biol Chem 263: 16303–16308.PubMedGoogle Scholar
  18. Larocca JN, Ledeen RW, Dvorkin B and Makman MH (1987) Muscarinic receptor binding and muscarinic receptor-mediated inhibition of adenylate cyclase in rat brain myelin. J Neurosci 7: 3869–3876PubMedGoogle Scholar
  19. Magee T and Hanley M (1988) Sticky fingers and CAAX boxes. Nature 335: 114–115PubMedCrossRefGoogle Scholar
  20. Mayorga LS, Diaz R and Stahl PD (1989) Regulatory role for GTP-binding proteins in endocytosis. Science 244: 1475–1477PubMedCrossRefGoogle Scholar
  21. Miller RJ (1988) G proteins flex their muscles (1988) Trends in Neurosci 11: 3–6.Google Scholar
  22. Nagata K, Nagao S and Nozawa Y (1989) Low Mr GTP-binding proteins in human platelets: cyclic AMP-dependent protein kinase phosphorylates m22KG(I) in membrane but not c21KG in cytosol. Biochem Biophys Res Comm 160: 235–242.PubMedCrossRefGoogle Scholar
  23. Norton WT, Poduslo SE (1973) Myelination in rat brain: method of myelin isolation. J Neurochem 21: 749–757PubMedCrossRefGoogle Scholar
  24. Ribeiro FAP and Rodbell M (1989) Pertussis toxin induces structural changes in Ga proteins independently of ADP-ribosylation. Proc Nat’l Acad Sci (USA) 86: 2577–2581CrossRefGoogle Scholar
  25. Santos E and Nebreda AR (1989) Structural and functional properties of ras proteins. The FASEB J 3: 2151–2163.Google Scholar
  26. Spiegel AM (1987) Signal transduction by guanine nucleotide binding proteins. Molec Cell Endocrin 49: 1–16CrossRefGoogle Scholar
  27. Sprinkle TJ and Hancock J (1989) Identification of phosphoryl and nucleotide binding sites on CNPase. Trans Amer Soc Neurochem 20: 255Google Scholar
  28. Towbin H, Staehlin T and Gordon J (1979) Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Nat Acad Sci USA 76: 4350–4354PubMedCrossRefGoogle Scholar
  29. Ulmer JB and Braun PE (1983) In vivo phosphorylation of myelin basic proteins in developing mouse brain: evidence that phosphorylation is an early event in myelin formation. Dev Neurosci 6: 345–355PubMedCrossRefGoogle Scholar
  30. Yong VW, Sekiguchi S, Kim MW and Kim SU (1988) Phorbol ester enhances morphological differentiation of oligodendrocytes in culture. J Neurosci Res 19: 187–194.PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • Peter E. Braun
    • 1
  • Lise Bernier
    • 1
  1. 1.Department of BiochemistryMcGill UniversityMontrealCanada

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