The Cytoskeleton and Polyspermy in Sea Urchin Eggs

  • L. Santella
Conference paper
Part of the NATO ASI Series book series (volume 45)


At fertilization the sea urchin egg undergoes a dramatic structural reorganization of its surface. Within the first few seconds there is a depolarization of the egg plasma membrane and this is followed by a transient increase in intracellular Ca++. The elevated Ca induces the exocytosis of cortical granules and the resulting fertilization membrane helps make the egg refractory to further insemination.


Cortical Actin Cortical Granule Cortical Reaction Rhodamine Phalloidin Cell BioI 
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  1. Allen R, Hagström B (1955) Interruption of the cortical reaction by heat. Exp Cell Res 9:157–167PubMedCrossRefGoogle Scholar
  2. Begg DA, Rebhurn LI (1979) pH regulates the polymerization of actin in the sea urchin egg cortex. J Cell Biol 83: 241–248PubMedCrossRefGoogle Scholar
  3. Begg DA, Rebhun LI, Hyatt H (1982) Structural organization of actin in the sea urchin egg cortex: microvillar elongation in tha absence of actin filament bundle formation. J Cell Biol 93: 24–32PubMedCrossRefGoogle Scholar
  4. Boveri T (1901) Die Polaritat von Ovocyte, Ei und Larvae des Strongylocentrotus lividus. Zool Jb (Morph) 14: 630–651Google Scholar
  5. Carron CP, Longo FJ (1982) Relation of cytoplasmic alkalinization to microvillar elongation and microfilament formation in the sea urchin egg. Dev Biol 89: 128–137PubMedCrossRefGoogle Scholar
  6. Cline CA, Shatten G (1986) Microfilaments during sea urchin fertilization: Fluorescence detection with rhodaminyl phalloidin. Gamete Res 14: 277–291PubMedCrossRefGoogle Scholar
  7. Dale B (1985) Sperm receptivity in sea urchin oocytes and eggs. J Exp Biol 118: 85–97Google Scholar
  8. Dale B (1987) Mechanism of fertilization Nature Lond 325: 762–763PubMedCrossRefGoogle Scholar
  9. Dale B, De Santis A (1981) Maturation and fertilization of the sea urchin oocyte: an electrophysiological study. Develop Biol 85:474–484PubMedCrossRefGoogle Scholar
  10. Dale B, Santella L (1985) Sperm-oocyte interaction in the sea urchin. J Cell Sci 74: 153–167PubMedGoogle Scholar
  11. Dale B, Hagström B, Santella L (1989) Partially fertilized sea urchin eggs: an electrophysiological and morphological study. Develop Growth & Differ 31: (2) 165–170CrossRefGoogle Scholar
  12. Eddy EM, Shapiro BM (1976) Changes in the topography of the sea urchin after fertilization. J Cell Biol 71: 35–48PubMedCrossRefGoogle Scholar
  13. Epel D (1978) Mechanism of activation of sperm and egg during fertilization of sea urchin gametes. Curr Top Dev Biol 12: 185–246PubMedCrossRefGoogle Scholar
  14. Hagström B, Runnström J (1959) Re-fertilization of partially fertilized sea urchin eggs. Protoplasma 87: 281–290CrossRefGoogle Scholar
  15. Hamaguchi Y, Mabuchi I (1988) Accumulation of fluorescent labeled actin in the cortical layer in sea urchin eggs after fertilization. Cell Motility & Cytoskeleton 9: 153–163CrossRefGoogle Scholar
  16. Henson JH, Begg DA (1988) Filamentous actin organization in the unfertilized sea urchin egg cortex. Dev Biol 127: 338–348PubMedCrossRefGoogle Scholar
  17. Longo F, Lynn J, McCulloh D, Chambers E (1986) Correlative ultrastructure and electrophysiological study of sperm egg interactions of the sea urchin Lytechinus variegatus. Develop Biol 118: 155–166PubMedCrossRefGoogle Scholar
  18. Rothschild L, Swann M (1952) The fertilization reaction in the sea urchin. The block to polyspermy. J Exp Biol 29: 469–483Google Scholar
  19. Runnström J (1961) The mechanism of protection of the eggs against polyspermy. Experiments on the sea urchin Paracentrotus lividus. Ark Zool 13: 565–571Google Scholar
  20. Santella L, Monroy A (1989) Cold shock induces actin reorganization and polyspermy in sea urchin eggs. J Exp Zool 252:183–189PubMedCrossRefGoogle Scholar
  21. Sardet C (1984) The ultrastructure of the sea urchin eggs cortex isolated before and after fertilization Dev Biol 105: 196–210PubMedCrossRefGoogle Scholar
  22. Shroeder T (1978) Microvilli on sea urchin eggs: A second burst of elongation. Dev Biol 64: 324–346Google Scholar
  23. Shroeder T (1979) Surface area change at fertilization: resorption of the mosaic membrane. Dev Biol 70: 306–326CrossRefGoogle Scholar
  24. Shroeder T, Stricker S (1983) Morphological changes during maturation of starfish oocytes: Surface ultrastructure and cortical actin. Develop Biol 98: 373–384CrossRefGoogle Scholar
  25. Spudich A, Spudich J (1979) Actin in triton-treated cortical preparations of unfertilized and fertilized sea urchin eggs. J Cell Biol 82: 212–226PubMedCrossRefGoogle Scholar
  26. Tilney LG, Jaffe LA (1980) Actin, microvilli and the fertilization cone of sea urchin eggs. J Cell Biol 87: 771–782PubMedCrossRefGoogle Scholar
  27. Wang Y, Taylor D (1979) Distribution of fluorescent labeled actin in living sea urchin eggs during early development. J Cell Biol 82: 672–679CrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • L. Santella
    • 1
  1. 1.Stazione Zoologica “A. Dohrn” di NapoliNapoliItaly

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