Human Sperm-Oocyte Fusion

  • A. Henry Sathananthan
  • S. C. Ng
  • A. O. Trounson
  • S. S. Ratnam
  • T. A. Bongso
Conference paper
Part of the NATO ASI Series book series (volume 45)

Abstract

Sperm-oocyte fusion is the key event of fertilization in the human, as well as in animals. It is a classic example of spontaneous membrane fusion between 2 cells without the intervention of agents such as viruses or chemicals. The process of gamete fusion has been extensively studied in various animals, including mammals (see reviews, Yanagimachi, 1981, 1988a,b; Bedford, 1983; Monroy, 1985; Longo, 1989), but there is little information about gamete fusion in the human (Soupart, 1980; Sathananthan and Chen, 1986; Sathananthan et al, 1986a, 1987). Yanagimachi (1988a,b) has comprehensively reviewed sperm-egg fusion in animals. This chapter will concentrate on what is known in the human with reference to recent reviews of gamete membrane fusion in mammals. The recent impact of IVF and new technologies of assisted reproduction such as microinsemination sperm transfer (MIST) beneath the zona, using a micromanipulator (Ng et al, 1988, 1989a) have further helped us understand the events that lead up to gamete fusion and microfertilization (Trounson, 1987; Laws-King et al, 1987; Sathananthan et al, 1989).

Keywords

Germinal Strontium Mist Hyaluronidase Eutherian Mammal 

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References

  1. Aitken RJ, Ross A, Lees MM (1983) Analysis of sperm function in Kartegener’s syndrome. Fertil steril 40: 696 – 698PubMedGoogle Scholar
  2. Bedford JM (1983) Form and function of eutherian spermatozoa in relation to the nature of egg vestments. In: Beier HM, Lindner HR (eds) Fertilization of the human egg In Vitro Springer-Verlag, Berlin, p133CrossRefGoogle Scholar
  3. Bedford JM, Moore HDM, Franklin LE (1979) Significance of the equatorial segment of the acrosome of the spermatozoon in eutherian mammals. Exp Cell Res 119:119–126PubMedCrossRefGoogle Scholar
  4. Bongso A, Sathananthan H, Wong PC, Ratnam SS, Ng SC, Anandakumar C, Ganatra S (1989) Human fertilization by microinjection of immotile sperm. Human Reprod 4: 175–179Google Scholar
  5. Chen C, Sathananthan AH (1986) Early penetration of human sperm through the vestments of human eggs in vitro. Arch Androl 16: 183–197PubMedCrossRefGoogle Scholar
  6. Dvorak M, Tesarik J, Kopechny V (1984) Ultrastructure of human fertilization In: Van Blerkom J, Motta PM (eds) Ultrastructure of reproduction, Martinus Nijhoff, Boston, p. 176CrossRefGoogle Scholar
  7. Edwards RG (1980) Conception in the human female. Academic Press, London.Google Scholar
  8. Gaddum — Rosse P (1985) Mammalian gamete interactions: What can be gained from observations on living eggs? Am J Anat 174: 347–356CrossRefGoogle Scholar
  9. Gwatkin RBL (1977) Fertilization mechanisms in man and mammals, Plenum Press, New York.Google Scholar
  10. Jaffe LA, Cross NL (1986) Electrical regulation of sperm — egg fusion. Annu Rev Physiol 48: 191–200PubMedCrossRefGoogle Scholar
  11. Laws-King A, Trounson A, Sathananthan H, Kola I (1987) Fertilization of human oocytes by micro injection of a single spermatazoon under the zone pellucida. Fertil Steril 48: 637–642PubMedGoogle Scholar
  12. Longo FJ (1989). Egg cortical architecture. In: Schatten H, Schatten G (eds) The cell biology of fertilization, Academic Press, San Diego, p105Google Scholar
  13. Lopata A, Sathananthan AH, McBain JC, Johnston WIH, Spiers AL (1980) The ultrastructure of the preovulatory human egg fertilized in vitro. Fertil Steril 33:12–20PubMedGoogle Scholar
  14. Lopata A, Nayudu P, Jones G, Abramczuk J (1985) The quality of human embryos obtained by IVF. In: Testart J, Frydmann R (eds) Human in victro fertilization. Actual problems and prospects, Elsevier, Armsterdam, p171Google Scholar
  15. Meizel S (1985) Molecules that initiate or help stimulate the acrosome reaction by their interaction with the mammalian sperm surface. Am J Anat 174: 285–302PubMedCrossRefGoogle Scholar
  16. Monroy A (1985) Processes controlling sperm-egg fusion. Eur J Biochem 152: 51–56PubMedCrossRefGoogle Scholar
  17. Mortimer D, Curtis EF, Dravland JE (1986) The use of strontium-substituted media for capacititing human spermatozoa: an improved sperm preparation method for zona-free hamsters egg penetration test. Fertil Steril 46: 97–103PubMedGoogle Scholar
  18. Nagae T, Yanagimachi R, Srivastava PN, Yanagimachi H (1986) Acrosome reaction in human spermatozoa. Fertil Steril 45: 701–707PubMedGoogle Scholar
  19. Ng SC, Sathananthan AH, Edirisinghe WR, Kum Chue JH, Wong PC, Ratnam SS, Sarla G (1987),. Fertilization of a human egg with sperm from a patient with immotile cilia syndrome: case report. In: Ratnam SS, Teoh ES, Anandakumar C (eds) Advances in Fertility and Sterility, vol 4, Parthenon, Lancaster, p71Google Scholar
  20. Ng SC, Bongso A, Ratnam SS, Sathananthan AH, Chan CL, Wong PC, Hagglund L, Anandakumar C, Wong YC and Goh VHH (1988) Pregnancy after transfer of multiple sperm under the zona. Lancet 2: 790.PubMedCrossRefGoogle Scholar
  21. Ng SC, Bongso A, Sathananthan AH, Ratnam SS (1989a) Micromanipulation: Its relevance to human in vitro fertilization. Fertil Steril (in press)Google Scholar
  22. Ng SC, Sathananthan AH, Bongso TA, Tok VCN, Ho JCK, Ratnam SS (1989b) Sperm transfer by micromainpulation into early human embryos (in review)Google Scholar
  23. Sathananthan AH (1984) Ultrastructural morphology of ferilization and early cleavage in the human. In: Trounson A, Wood C (eds) In vitro fertilization and embryo transfer. Churchill Livingstone, Edinburgh, p131Google Scholar
  24. Sathananthan AH and Trounson AO (1982) Ultrastructure of cortical granule release and zona interaction in monospermic and Polyspermic human ova fertilized in vitro. Gamete Res 6: 225–234CrossRefGoogle Scholar
  25. Sathananthan AH and Trounson A (1985) The human pronuclear ovum: fine structure of monospermic and Polyspermic fertilization in vitro. Gamete Res 12:385–398CrossRefGoogle Scholar
  26. Sathananthan AH and Chen C (1986) Sperm-oocyte membrane fusion in the human during monospermic fertilization. Gamete Res 15:177–186CrossRefGoogle Scholar
  27. Sathananthan AH and Trounson A (1989) The microinjection technique and the role of the acrosome reaction in microfertilization. In: Ben-Rafael Z (ed) In vitro fertilization and alternate assisted reproduction, Plenum, New York (in press)Google Scholar
  28. Sathananthan AH, Trounson AO, Wood C, Leeton JF (1982) Ultrastructural observations on the penetration of human sperm into the zona pellucida of the human egg in vitro. J Androl 3:356–364Google Scholar
  29. Sathananthan AH, Ng SC, Edirisinghe R, Ratnam SS, Wong PC (1986a) Sperm-egg interaction in vitro. Gamete Res 15:317–326CrossRefGoogle Scholar
  30. Sathananthan AH Trounson A and Wood C (1986b) Atlas of fine structure of human sperm penetration eggs and embryos cultured in vitro. Praeger Scientific, PhiladelphiaGoogle Scholar
  31. Sathananthan AH, Trounson A, Freemann L (1987) Morphology and fertilizability of frozen human oocytes. Gamete Res 16: 343–354PubMedCrossRefGoogle Scholar
  32. Sathananthan AH, Ng SC, Trounson A, Bongso A, Laws-King A, Ratnam SS (1989) Human micro-insemination by injection of single or multiple sperm: ultrastructure. Human Reprod 4:574–383Google Scholar
  33. Soupart P (1980) Fertilization. In: Hafez ESE (ed) Human reproduction: conception and contraception. Harper & Row, New York, p453Google Scholar
  34. Soupart P, Strong PA (1974) Ultrastructural observations on human oocytes fertilized in vitro. Fertil Steril 25:11–44PubMedGoogle Scholar
  35. Soupart P, Strong PA (1975) Ultrastructural observations on Polyspermic penetration of zona pellucida free human oocytes inseminated in vitro. Fertil Steril 36:523–537Google Scholar
  36. Trounson A (1987) Microfertilization. Proc Fifth World Congress In Vitro Fertilization, Norfolk, Virginia (abstract)Google Scholar
  37. Trounson A (1989) Fertilization and embryo culture. In: Wood C, Trounson A (eds) Clinical in vitro fertilization, 2nd ed, Springer-Verlag, London, p32Google Scholar
  38. Usui N, Yanagimachi R (1976) Behavior of hamster sperm nuclei incorporated into eggs at variours stages of maturation, fertilization and early development. J Ultrastructure Res 57: 276–288CrossRefGoogle Scholar
  39. Wassarman PM, Florman HM, Greve JM (1985) Receptor mediated sperm-egg interactions in mammals. In: Metz CB, Monroy A (eds) Fertilization, Vol 2, Academic Press, New York, p341Google Scholar
  40. Yanagimachi R (1981) Mechanisms of fertilization in mammals. In Mastroianni L Jr, Biggers JD (eds) Fertilization and embryonic development in vitro. Plenum Press, New York, p 81Google Scholar
  41. Yanagimachi R (1988a) Sperm-egg fusion. In: Duzgunes N, Bronner F (eds) Vol 32, Membrane fusion in fertilization, cellular transport and viral infection, Academic Press, New York, p 3Google Scholar
  42. Yanagimachi R (1988b) Mammalian fertilization. In: Knobil E, Neill J et al (eds) The physiology of reproduction, Raven Press, New York, p 135Google Scholar
  43. Zamboni L (1971) Fine morphology of mammalian fertilization. Harper and Row, New YorkGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1990

Authors and Affiliations

  • A. Henry Sathananthan
    • 1
    • 2
  • S. C. Ng
    • 1
  • A. O. Trounson
    • 1
  • S. S. Ratnam
    • 1
  • T. A. Bongso
    • 1
  1. 1.Monash Medical CentreMelbourne and National University HospitalSingapore
  2. 2.Lincoln School of Health SciencesLa Trobe UniversityCarltonAustralia

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