Localization of Hormones by Antibodies

  • P. U. Heitz
Conference paper
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 118)

Abstract

Hormones are substances produced by endocrine cells which are carried by the blood from the site of their secretion to the receptors of their target cells. At present hormones, including paracrine and autocrine substances, and neural messengers form the group of regulatory substances. Chemically they are peptides, proteins, glycoproteins, steroid hormones, iodothyronines or biogenic amines. Antibodies can be raised against many, if not all hormones, but the immunogenicity of the different substances varies widely.

Keywords

Formaldehyde Citrate Titration Adenoma Polypeptide 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Bishop AE, Power RF, Polak JM (1988) Markers for neuroendocrine differentiation. Pathol Res Pract 183: 119–128PubMedCrossRefGoogle Scholar
  2. Chejfec G, Falkmer S, Askensten U, Grimelius L, Gould VE (1988) Neuroendocrine tumors of the gastrointestinal trat. Pathol Res Pract 183: 143–154PubMedCrossRefGoogle Scholar
  3. Danscher G (1981) Localization of gold in biological tissue. A photochemical method for light and electron microscopy. Histochemistry 71: 81–88PubMedCrossRefGoogle Scholar
  4. Danscher G, Nörgaard JOR (1983) Light microscopic visualization of colloidal gold on resin-embedded tissue. J Histochem Cytochem 31: 1394–1398PubMedCrossRefGoogle Scholar
  5. Falkmer S, Hakanson R, Sundler F (eds) (1984) Evolution and tumour pathology of the neuroendocrine system. Elsevier, AmsterdamGoogle Scholar
  6. Guesdon JL, Ternynck T, Avrameas S (1979) The use of avidin-biotin interaction in immunoenzymatic techniques. J Histochem Cytochem 27: 1131–1139PubMedCrossRefGoogle Scholar
  7. Heitz PU (1986) Hormonale Tumormarker. Verh Dtsch Ges Pathol 70: 64–81PubMedGoogle Scholar
  8. Heitz PU (1987) Neuroendocrine tumor markers. In: Seifert G (ed) Morphological tumor markers. General aspects and diagnostic relevance. Springer, Berlin Heidelberg New York, pp 279–306 (Current topics in pathology vol 77 )Google Scholar
  9. Heitz PU, Klöppel G (1987) Endokrine Tumoren des Pankreas and des Duodenum. Verh Dtsch Ges Pathol 71: 202–221PubMedGoogle Scholar
  10. Heitz PU, Steiner H, Halter F, Egli F, Kapp JP (1971) Multihormonal amyloid-producing tumour of the islets of Langerhans in a twelve year old boy. Virchows Arch [A] 353: 312–324Google Scholar
  11. Heitz PU, Kasper M, Klöppel G, Polak JM, Vaitukaitis JL (1983) Glycoprotein-hormone alpha-chain production by pancreatic endocrine tumors: a specific marker for malignancy. Immunocytochemical analysis of tumors of 155 patients. Cancer 51: 277–282PubMedCrossRefGoogle Scholar
  12. Heitz PU, Landolt AM, Zenklusen HR, Kasper M, Reubi JC, Oberholzer M, Roth J (1987a) Immunocytochemistry of pituitary tumors. J Histochem Cytochem 35: 1005–1011PubMedCrossRefGoogle Scholar
  13. Heitz PU, von Herbay G, Klöppel G, Komminoth P, Kasper M, Höfler H, Müller KM, Oberholzer M (1987b) The expression of subunits of human chorionic gonadotropin (hCG) by nontrophoblastic, nonendocrine, and endocrine tumors. Am J Clin Pathol 88: 467–472PubMedGoogle Scholar
  14. Holgate CS, Jackson P, Cowen PN, Bird CC (1983) Immunogold silver staining: new method of immunostaining with enhanced sensitivity. J Histochem Cytochem 31: 938–944PubMedCrossRefGoogle Scholar
  15. Kelly B (1985) Pathways of protein secretion in eukaryotes. Science 230: 25–32PubMedCrossRefGoogle Scholar
  16. Klöppel G, Heitz PU (1988) Pancreatic endocrine tumors. Pathol Res Pract 183: 155–168PubMedCrossRefGoogle Scholar
  17. Polak JM, Bloom SR, Adrian TE, Heitz PU, Bryant MG, Pearse AGE (1976) Pancreatic polypeptide in insulinomas, gastrinomas, vipomas, and glucagonomas. Lancet 1: 328–330PubMedCrossRefGoogle Scholar
  18. Roth J, Kasper M, Heitz PU, Labat F (1985) What’s new in light and electron microscopic immunocytochemistry? Application of the protein A-gold technique to routinely processed tissue. Pathol Res Pract 180: 711–717PubMedCrossRefGoogle Scholar
  19. Ryffde Lèche A, Staub JJ, Kohler-Faden R, Müller-Brand J, Heitz PU (1986) Thyroglobulin production by malignant thyroid tumors: an immunocytochemical and radioimmunoassay study. Cancer 57: 1045–1053Google Scholar
  20. Sternberger LA (1986) Immunocytochemistry, 3rd edn. Wiley, New YorkGoogle Scholar
  21. Taatjes DJ, Chen TH, Ackerström B, Björck L, Carlemalm E, Roth J (1987) Streptococcal protein G-gold complex: comparison with staphylococcal protein A-gold complex for spot blotting and immunolabeling. Eur J Cell Biol 45: 151–159PubMedGoogle Scholar

Copyright information

© Springer-Verlag Berlin·Heidelberg 1990

Authors and Affiliations

  • P. U. Heitz
    • 1
  1. 1.Institut für PathologieUniversität ZürichZürichSwitzerland

Personalised recommendations