Studies on the Nature of Physiologically Processed Antigen

  • S. Demotz
  • H. M. Grey
  • A. Sette
Conference paper


Activation of class II-restricted T cells requires the formation of a trimolecular complex between antigenic peptide fragments, class II MHC molecules, and the T cell receptor (Buus et al. 1987). In contrast to the substantial body of evidence characterizing the interaction between synthetic peptides and class II molecules, essentially no data are available on the nature of the antigen resulting from in vivo processing of protein molecules. It has been postulated that fragments arise from limited proteolytic degradation of native antigens inside acidic compartments of the antigen-presenting cells; however, up to now no direct chemical characterization of physiologically processed peptides has been presented. In this paper we review experiments that we have performed that provide the first partial characterization of a physiologically processed antigen.


Antigenic Peptide Planar Membrane Acidic Compartment Restricted Determinant Antigenic Material 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.


Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.


  1. Adorini L, Sette A, Buus S, Grey HM, Darsley M, Lehmann PV, Doria G Nagy ZA, Appella E (1988) Interaction of an immunodominant epitope with la molecules in T-cell activation. Proc Natl Acad Sci USA 85:5181.PubMedCentralPubMedCrossRefGoogle Scholar
  2. Buus, S, Sette A, Colón SM, Jenis DM, and Grey HM (1986) Isolation and characterization of antigen-Ia complexes involved in T cell recognition. Cell 47:1071.PubMedCrossRefGoogle Scholar
  3. Buus, S, Sette A, and Grey, HM (1987) The interaction between protein-derived immunogenic peptides and Ia. Immunol. Rev. 98:115.PubMedCrossRefGoogle Scholar
  4. Buus, S, Sette A, Colón SM, and Grey HM (1988) Autologous peptides constitutively occupy the antigen binding site on Ia. Science 242:1045.PubMedCrossRefGoogle Scholar
  5. Falo, LD Jr, Benacerraf B, and Rock KL (1986) Phospholipase treatment of accessory cells that have been exposed to antigen selectively inhibits antigen-specific Ia-restricted, but not allospecific, stimulation of T lymphocytes. Proc Natl Acad Sci USA 83:6994.PubMedCentralPubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • S. Demotz
  • H. M. Grey
  • A. Sette

There are no affiliations available

Personalised recommendations