Studies on the Nature of Physiologically Processed Antigen
Activation of class II-restricted T cells requires the formation of a trimolecular complex between antigenic peptide fragments, class II MHC molecules, and the T cell receptor (Buus et al. 1987). In contrast to the substantial body of evidence characterizing the interaction between synthetic peptides and class II molecules, essentially no data are available on the nature of the antigen resulting from in vivo processing of protein molecules. It has been postulated that fragments arise from limited proteolytic degradation of native antigens inside acidic compartments of the antigen-presenting cells; however, up to now no direct chemical characterization of physiologically processed peptides has been presented. In this paper we review experiments that we have performed that provide the first partial characterization of a physiologically processed antigen.
KeywordsCholesterol HPLC Filtration Lymphoma EDTA
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