Characterization of the Secondary Antibody Response Using Purified Memory B and T Cells
Multicolor immunofluorescence analysis of antigen binding cells allows us to detect and purify memory B cells in a highly specific manner. We use the fluorescent protein PE (phycoerythrin) as an antigen in order to reveal antigen binding cells (PE+++) (Hayakawa, 1987) and simultaneously permit analysis of the expression of other cell surface molecules. In addition, we can resolve a memory T cell subset which provides help for antibody secretion in the secondary response from the total CD4+ T cell population by expression of particular determinants (Hayakawa, 1988). Using this approach, we find that the secondary antibody response by long-term memory B and T cells can be uniquely characterized as follows.
KeywordsAntigen Binding Antibody Secretion Surface Immunoglobulin Antibody Secreting Cell Antigen Priming
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
Black SJ, van der Loo W, Loken MR, Herzenberg LA (1977) Expression of IgD by murine lymphocytes. Loss of surface IgD indicates maturation of memory B cells. J Exp Med 147:984–996CrossRefGoogle Scholar
Coffman R (1982) Surface antigen expression and immunoglobulin gene rearrangement during mouse pre-B cell development. Immunol Rev 69:5–23PubMedCrossRefGoogle Scholar
Hardy RR, Hayakawa K, Parks DR, Herzenberg LA, Herzenberg LA (1984) Murine B cell differentiation lineages. J Exp Med 159:1169–1188PubMedCrossRefGoogle Scholar
Hayakawa K, Ishii R, Yamasaki K, Kishimoto T, Hardy RR (1987) Isolation of high-affinity memory B cells: Phycoerythrin as a probe for antigen-binding cells. Proc Natl Acad Sci USA 84:1379–1383PubMedCentralPubMedCrossRefGoogle Scholar
Hayakawa K, Hardy RR (1988) Murine CD4+ T cell subsets defined. J Exp Med 168:1825–1838PubMedCrossRefGoogle Scholar
Hayakawa K, Hardy RR (1989) Phenotypic and functional alteration of CD4+ T cells after antigen stimulation. Resolution of two populations of memory T cells that both secrete interleukin 4. J Exp Med 169:2245–2250PubMedCrossRefGoogle Scholar
© Springer-Verlag Berlin Heidelberg 1989