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Characterization of the Secondary Antibody Response Using Purified Memory B and T Cells

  • K. Hayakawa
  • R. R. Hardy

Abstract

Multicolor immunofluorescence analysis of antigen binding cells allows us to detect and purify memory B cells in a highly specific manner. We use the fluorescent protein PE (phycoerythrin) as an antigen in order to reveal antigen binding cells (PE+++) (Hayakawa, 1987) and simultaneously permit analysis of the expression of other cell surface molecules. In addition, we can resolve a memory T cell subset which provides help for antibody secretion in the secondary response from the total CD4+ T cell population by expression of particular determinants (Hayakawa, 1988). Using this approach, we find that the secondary antibody response by long-term memory B and T cells can be uniquely characterized as follows.

Keywords

Antigen Binding Antibody Secretion Surface Immunoglobulin Antibody Secreting Cell Antigen Priming 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

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Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • K. Hayakawa
  • R. R. Hardy

There are no affiliations available

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