Abstract
Two T cell populations exist which express distinct T cell receptors (TCR), TCR a 13 (Marrack and Kappler, 1986) and TCR γδ (Brenner et al. 1986). T cells bearing the TCR αβ complex are numerically predominant in the blood and organized lymphoid organs and recognize foreign peptides presented in the context of self-major histocompatibility complex (MHC) molecules. As mature lymphocytes, they are “single positive” and express either CD4 or CD8 which participate in recognition of MHC class II or class I molecules, respectively. In contrast, the recognition specificity of lymphocytes bearing the γδ-TCR are unknown, and these cells are frequently both CD4 and CD8 negative (“double negative”) (Groh et al. 1988). Besides γδ-TCR lymphocytes, a population of CD-4- lymphocytes bearing the αβ-TCR also exists in man (Shivakumar et al. 1989). In cytofluorographic analyses, staining with anti-CD4 together with anti-CD8 in one color compared to staining with anti-CD3 in a second color, we observed the expected presence of a subpopulation of CD3+CD-8- cells in human peripheral blood. When also staining with mAb anti-TCRδ1 (pan TCR γδ reactive), a majority of these double negative T cells were shown to bear the γδ -TCR. However, in all normal subjects examined a population of 0.5–2.0% of these CD4-8- T cells was found to lack reactivity with anti-TCRδ1. These cells were isolated and were shown to express the αβ-TCR. CD4-8- TCR-αβ bearing cells have been reported to be increased in number in certain genetically autoimmune strains of mice that have generalized lymphadenopathy (gld, L pr) (Miescher et al. 1987). These mice share some clinical features in common with the human autoimmune disease systemic lupus erythematosus (SLE) where an increase in the number of CD4”8” T cells in peripheral blood has also been found (Shivakumar et al. 1989). Our analyses suggest that the number of double negative αβ-TCR bearing lymphocytes were increased and represented more than 10% of the CD3+ cells in several individuals with SLE. Thus the CD3+4-8- phenotype occurs on a majority of αβ-TCR lymphocytes and on a small fraction of αβ-TCR bearing lymphocytes, particularly in SLE.
Keywords
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.
This is a preview of subscription content, log in via an institution.
Buying options
Tax calculation will be finalised at checkout
Purchases are for personal use only
Learn about institutional subscriptionsPreview
Unable to display preview. Download preview PDF.
References
Bahn, A.K., E.L. Reinherz, Poppama S., R.T. McGluskey, S.F. Schlossman (1980) J. Exp. Med. 152:771.
Balk, S.P., Bleicher, P.A., Terhorst, C. (1989) Proc. natn. Acad. Sci. U.S.A. 86:252. 103.
Bluestone, J.A. and Matis, L.A. J. Immunol. (1989) 142:1785.
Borst, J. et al. (1987) Nature 325:683.
Brenner, M.B. et al. (1987) Nature 325:689.
Brenner, M.B., Strominger, J.L. & Krangel, M.S. (1988) Adv. Immun. 43:133.
Brenner, M.B., McLean, J., Dialynas, D.P., Strominger, J.L., Smith, J.A., Owen, F.L., Seidman, J.G., Ip, S., Rosen, F., Krangel, M.S. (1986) Nature 322:145.
Calabi F. and Milstein, C. (1986) Nature 323:540.
Fithian, E. et al. (1981) Proc. natn. Acad. Sci. U.S.A. 78:2541.
Groh, V., Porcelli, S., Fabbi, M., Lanier, L.L., Picker, L.J., Anderson, T., Warnke, R.A., Bhan, A.K., Strominger, J.L., Brenner, M.B. (1989) J. Exp. Med. 169:1277.
Marrack, P., and Kappler, J. (1986) Adv. Immunol. 38:1.
Martin L.H., Calabi, F., Milstein, C. (1986) Proc. natn. Acad. Sci. U.S.A. 83:9154.
Martin L.H., Calabi, F., Lefebvre, F.-A., Bilsland, C.A.G., Milstein, C. (1987) Proc. natn. Acad. Sci. U.S.A. 84:9189.
McMichael, A.J., Pilch, J.R., Galfre, G., Mason, D.Y., Fabre, J.W., Milstein, C. (1979) Eur J. Immunol. 9:205.
Miescher, G.C., Budd, R.C., Lees, R.K. & MacDonald, H.R. (1987) J.Immunol. 138:1959.
Porcelli, P., Brenner, M.B., Greenstein, J.L., Balk, S.P., Terhorst, C., Bleicher, PA. (1989) Nature, In Press.
Reinherz E.L., S.F. Schlossman (1980) Cell 19:821.
Shivakumar, S., Tsokos, G.C. & Datta, S.K. (1989) J. Immun. 143:103.
Small, T.N. et al. (1987) J. Immun. 138:2864.
Terhorst, C., A van Agthoven, K. LeClair, P. Snow, E.L. Reinherz, S.F. Schlossman (1981) Cell 23:771.
van Agthoven, A. C. Terhorst (1982) J. Immunol. 128:426.
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1989 Springer-Verlag Berlin Heidelberg
About this paper
Cite this paper
Porcelli, S., Bleicher, P.A., Greenstein, J.L., Balk, S.P., Terhorst, C., Brenner, M.B. (1989). Lymphocytes Bearing Either γδ-TCR or αβ-TCR Can Recognize Non-MHC Encoded CD1 Molecules. In: Melchers, F., et al. Progress in Immunology. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83755-5_5
Download citation
DOI: https://doi.org/10.1007/978-3-642-83755-5_5
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-83757-9
Online ISBN: 978-3-642-83755-5
eBook Packages: Springer Book Archive