Development of the T Cell Repertoire: Contributions of Both TCR-MHC and Accessory Molecule-MHC Interactions

  • L. A. Jones
  • J. C. Zúñiga-Pflücker
  • J. S. Fine
  • D. L. Longo
  • A. M. Kruisbeek
Conference paper

Abstract

To examine the issue of thymic selection with experimental systems entirely different from hematopoietic and thymic chimeras, the development of the T cell repertoire has been analyzed under conditions where expression of MHC- encoded gene products is blocked by in vivo mAb treatment during the early post-natal period. Such experiments also serve to re-evaluate in normal mice whether positive selection in the context of thymic MHC indeed forms the basis for the phenomenon of MHC restriction. The initial results demonstrated that blocking of class ll-MHC resulted in abrogation of development of CD4+CD8- T cells (Kruisbeek et al 1985), and blocking of class l-MHC prevented generation of CD4-CD8+ T cells (Marusic- Galesic et al 1988). Thus, the impressively strong correlation, in mature T cells, between expression of particular accessory molecules (CD4 or CD8) and MHC- restriction specificity (class II or class I, respectively) (Swain 1983) appears to reflect early selection events. These findings are consistent with the idea that blocking of the appropriate MHC molecules prevents delivery of a positive signal necessary for clonal differentiation.

Keywords

Hydrocortisone 

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References

  1. Berg LJ, Pullen AM, Fazekas de St Groth B, Mathis D, Benoist C, Davis MM (1989) Cell In PressGoogle Scholar
  2. Bill J, Kanagawa O, Woodland DL, Palmer E (1989) J Exp Med 169:1405–1419PubMedCrossRefGoogle Scholar
  3. Blackman MA, Marrack P, Kappler J (1989) Science 244:214–217PubMedCrossRefGoogle Scholar
  4. Crispe IN, and Bevan MJ (1987) J Immunol 138:2013–2018PubMedGoogle Scholar
  5. Emmrich F, Kanz L, Eichmann K (1987) Eur J Immmunol 17:529–535CrossRefGoogle Scholar
  6. Fazekas de St Groth B, Gallagher PF, Miller JFAP (1986) Proc Natl Acad Sci USA 83:2594–2597CrossRefGoogle Scholar
  7. Fowlkes BJ, Schwartz RH, Pardoll DM (1988) Nature 334:620–623PubMedCrossRefGoogle Scholar
  8. Janeway CA (1988) Nature 335:208–210PubMedCrossRefGoogle Scholar
  9. Janeway CA (1989) Immunol. Today 10:234–238PubMedCrossRefGoogle Scholar
  10. Kappler JW, Roehm N, Marrack P (1987) Cell 49:273–280PubMedCrossRefGoogle Scholar
  11. Kappler JW,Wade T, White J, Kushnir E, Blackman M, Bill, Roehm N, Marrack P Cell 49:263–271Google Scholar
  12. Kappler JW, Kushnir E, Marrack(1989) J Exp Med 169:1533–1541PubMedCrossRefGoogle Scholar
  13. Kruisbeek AM, Fowlkes BJ, Bridges SA, Mond JJ, Longo DL (1985) J Exp Med 161:1029–1039PubMedCrossRefGoogle Scholar
  14. Liao NS, Maltzman J, Raulet DH (1989) J Exp Med 170:135–143PubMedCrossRefGoogle Scholar
  15. MacDonald HR, Lees RK, Schneider R, Zinkernagel RM, Hengartner H (1988a) Nature 336:471–473PubMedCrossRefGoogle Scholar
  16. MacDonald HR, Hengartner H, Pedrazzini (1988b) Nature 335:174–176PubMedCrossRefGoogle Scholar
  17. Marrack P, Lo D, Brinster R, Palmiter R, Burkly L, Flavell RH, Kappler J (1988) Cell 53:627–634PubMedCrossRefGoogle Scholar
  18. Marrack P, Kappler J (1988) Nature 332:840–843PubMedCrossRefGoogle Scholar
  19. Marusic-Galesic S, Stephany DA, Longo DL, Kruisbeek AM (1988) Nature 333:180–183PubMedCrossRefGoogle Scholar
  20. McCarthy SA, Kruisbeek AM, Uppenkamp IK, Sharrow SO, Singer A (1988) Nature 336: 76–78PubMedCrossRefGoogle Scholar
  21. Mizuochi T, Tentori L, Sharrow SO, Kruisbeek AM, Singer A (1988) J Exp Med 168:437–452PubMedCrossRefGoogle Scholar
  22. Rudd CE, Trevillyan JM, Dasgupta JD, Wong LL, Schlossman SF (1988) Proc Natl Acad Sci USA 85:5190–5194PubMedCentralPubMedCrossRefGoogle Scholar
  23. Schrezenmeier H, Fleischer B (1988) J Immunol 141:398–403PubMedGoogle Scholar
  24. Scott B, Bluthmann, Teh HS, von Boehmer H (1989) Nature 338:591–593PubMedCrossRefGoogle Scholar
  25. Sha W, Nelson C, Newberry R, Kranz D, Russell J, Loh D (1988) Nature 336:73–76PubMedCrossRefGoogle Scholar
  26. Swain S (1983) Immunol Rev 74:129–149PubMedCrossRefGoogle Scholar
  27. Teh HS, Kisielow P, Scott B, Hiroyuki K, Uematsu Y, Bluthmann H, von Boehmer H (1988) Nature 355:229–233CrossRefGoogle Scholar
  28. Veillette A, Bookman MA, Horak EM, Bolen JB (1988) Cell 55:301–309PubMedCrossRefGoogle Scholar
  29. Veillette A, Zúñiga-Pflücker JC, Bolen JB, Kruisbeek AM (1989) Submitted von Boehmer, H. (1986) Immunol. Today 7:333–738Google Scholar
  30. Weynand CM, Goronzy J, Fathman CG (1987) J Immunol 138:1351–1358Google Scholar
  31. Zuniga-PflGcker JC, McCarthy SA, Weston M, Longo DL, Singer A, Kruisbeek AM (1989a) J Exp Med 169:2085–2096CrossRefGoogle Scholar
  32. Zúñiga-Pflücker JC, Jones LA, Longo DL, Kruisbeek AM (1989b) Cold Spring Harbor Symposium “Immunologic Recognition “In PressGoogle Scholar
  33. Zúñiga-Pflücker JC, Jones LA, Longo DL, Kruisbeek AM (1989c) SubmittedGoogle Scholar
  34. Zúñiga-Pflücker JC, Longo DL, Kruisbeek AM (1989d) Nature 338:76–78PubMedCrossRefGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • L. A. Jones
  • J. C. Zúñiga-Pflücker
  • J. S. Fine
  • D. L. Longo
  • A. M. Kruisbeek

There are no affiliations available

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