Transgenic Plants and the Study of Plant Development
One of the most powerful means for approaching difficult biological problems is our ability to transform and observe directly the trait we are studying. This is as true in E. coli as in the study of one of the most complex of biological phenomena, the process of plant development. It is only during the past 5 years that plant molecular biologists have been able to transform and produce stable, transgenic plants which contain a wide variety of chimeric genes. Several recent reviews cover the many aspects associated with transgenic plants, including their use in the analysis of the molecular organization of plant genes (Schell 1987), and the study of plant gene expression (Kuhlemeier et al. 1987; Willmitzer 1988). This short review focuses on the current uses of transgenic plants for the purpose of studying plant development, in particular work we are presently engaged in. Specifically, this review will discuss the study of Agrobacterium oncogenic genes in transgenic tobacco, and the use of both transposons and T-DNA for insertional mutagenesis in tobacco and Arabidopsis.
KeywordsTransgenic Plant Hairy Root Transgenic Tobacco Insertional Mutagenesis Crown Gall
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- Depicker A, Montagu MV, Schell JS (1983) Plant cell transformation by Agrobacterium plasmids. In: Kosuge T, Meredith CP, Hollaender A (eds) Genetic Engineering of plants: an agricultural perspective. Plenum, New York, pp 143–176Google Scholar
- Fedoroff NV (1983) Controlling elements in maize. In: Shapiro J (ed) Mobile Genetic Elements. Academic Press, New York, pp 1–63Google Scholar
- Kahl G, Schell J (1982) Molecular biology of plant tumors. Academic Press, LondonGoogle Scholar
- Koncz C, Martini N, Koncz-Kalman Z, Olsson O, Rademiacher A, Szalay A, Schell J (1987) Genetic tools for the analysis of gene expression in higher plants. In: Bruening G, Harada J, Kosuge T, Hollaender A (eds) Tailoring genes for crop improvement. Plenum, New York, pp 197–209Google Scholar
- Koncz C, Marini N, Mayerhofer R, Koncz-Kalman Z, Körber H, Redei G, Schell J (1988) T-DNA insertions preferentially tag functional genes in Arabidopsis and Nicotiana. (submitted for publication)Google Scholar
- Schmülling T, Schell J, Spena A (1988) Single genes from Agrobacterium rhizogenes influence plant development. EMBO J EMBO J 7: 2621–2629Google Scholar