Fast Atom Bombardment Mass Spectrometry
Traditionally, a major constraint in the application of mass spectrometry to analytes of biological origin has been the need to transfer the analyte to the gas phase for ionization by the conventional techniques of electron ionization (EI) and chemical ionization (CI) (Busch and Cooks 1982). This requirement for volatilization has limited the range of applications of the combined technique of gas chromatography-mass spectrometry (GC-MS) (Busch and Glish 1984). Whereas derivatization of the analyte(s) can overcome these specific limitations of volatility in GC-MS applications, derivatization is often restricted to analytes of relatively low molecular weight, and each derivatization scheme may not be appropriate for all compounds within any given analyte class. For nonvolatile or thermally fragile samples, heating the sample to vaporize it often leads to thermal degradation (Busch and Cooks 1982). These widely recognized limitations have spurred many recent developments in liquid chromatography-mass spectrometry (LC-MS), specifically, the development of new interfaces for transferring LC effluent into a mass spectrometer, and alternative ionization techniques which overcome the need for volatility of the analyte (Busch and Cooks 1982; Busch and Glish 1984). Of the latter techniques, molecular secondary ion mass spectrometry (SIMS) (Benninghoven and Sichtermann 1977) and fast atom bombardment mass spectrometry (FAB-MS) [liquid SIMS] (Barber et al. 1981) have emerged as powerful new research tools in recent years. These methods are now in routine use in a wide range of biological applications (Rinehart 1982; Busch and Glish 1984).
KeywordsFast Atom Bombardment Betaine Aldehyde Fast Atom Bombardment Mass Spectrometry Fast Atom Bombardment Mass Spectrum Molecular Cation
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