DNA Repair by the Ada Protein of E. coli
The Ada protein of E. coli exemplifies the highly specialized nature of the enzymes which have evolved to repair DNA. This protein exhibits not only novel mechanistic features but also provides an apparently unique example of a strategy for controlling gene expression in E. coli. Furthermore, knowledge of the properties and mode of action of the Ada protein has afforded insight into how human cells are affected by alkylating agents, including those used in chemotherapy.
KeywordsToxicity Cysteine Proline Polypeptide Thiol
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- Ishizaki K, Tsujimura T, Yawata H, Fujio C, Nakabeppu U, Sekiguchi M, Ikenaga M (1986) Transfer of the Escherichia coli O6-methylguanine methyltransferase gene into repair-deficient human cells and restoration of cellular resistance to N-methyl-N’-nitro-N-nitrosoguanidine. Mutat Res 166:135–141PubMedGoogle Scholar
- Olsson M, Lindahl T (1980) Repair of alkylated DNA in Escherichia coli: methyl group transfer from O6-methylguanine to a protein cysteine residue. J Biol Chem 25 5:10569–10571Google Scholar
- Sedgwick B, Robins P, Totty N, Lindahl T (1987) Functional domains and methyl acceptor sites of the Escherichia coli Ada protein. J Biol Chem (in press)Google Scholar