Laser Raman Spectroscopy of Nucleic Acids

  • G. J. ThomasJr.
  • A. H.-J. Wang
Part of the Nucleic Acids and Molecular Biology book series (NUCLEIC, volume 2)


Recent applications of the technique of single crystal X-ray diffraction, in combination with structure refinement methods, have revealed architectural details of right- and left-handed DNA helices at atomic resolution. [For comprehensive reviews of this work, see the monograph edited by Jurnak and McPherson (Jurnak and McPherson 1985).] Structural details of DNA in complexes with regulatory proteins (Anderson et al. 1987) and anti-tumor drugs (Wang 1987) have also been probed by crystallographic methods. Together these studies provide a wealth of information about the conformational properties of DNA, including dependence upon base sequence and intercalating agents, influence of solvent and counterions, and complementarity between DNA and substrate surfaces. X-ray diffraction experiments which are currently in progress should provide still further information on the nature of DNA-substrate interactions, as well as new insight into long-range structural features, such as helix curvature.


Raman Spectrum Raman Spectroscopy Raman Band Backbone Conformation Helix Axis 
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Copyright information

© Springer-Verlag Berlin Heidelberg 1988

Authors and Affiliations

  • G. J. ThomasJr.
    • 1
  • A. H.-J. Wang
    • 2
  1. 1.Division of Cell Biology and Biophysics, School of Basic Life SciencesUniversity of Missouri-Kansas CityKansas CityUSA
  2. 2.Department of Physiology and BiophysicsUniversity of Illinois at Urbana-ChampaignUrbanaUSA

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