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Oxygen and Hydrogen Isotope Measurements in Plant Cellulose Analysis

  • L. Da Silveira Lobo Sternberg
Part of the Molecular Methods of Plant Analysis book series (MOLMETHPLANT, volume 10)

Abstract

Isotopes are atoms containing the same number of protons but a different number of neutrons in their nucleus. Most of us are familiar with unstable isotopes (e.g., Carbon-14) which decay to another element (e.g., Carbon-14 decays to Nitrogen-14). This chapter, however, is concerned with stable isotopes which do not decay and thus produce no radioactivity. The most abundant elements found in plant biomass have two or more stable isotopes. Carbon exists either as carbon-12 (6 protons and 6 neutrons) or carbon-13 (6 protons and 7 neutrons), hydrogen can be found either as deuterium (1 proton and 1 neutron) or protium (1 proton), and oxygen can be found either as oxygen-16 (8 protons and 8 neutrons), oxygen-17 (8 protons and 9 neutrons), or oxygen-18 (8 protons and 10 neutrons). Isotopes having the least number of neutrons are usually called the lighter isotopes, and those having more neutrons are called the heavier isotopes. The natural abundance of the isotope of those elements with the least mass (lower number of neutrons) is greater than those with the larger mass. Thus carbon in the biosphere is comprised of 98.89% carbon 12 and only 1.11% of carbon-13 (Nier and Gulbransen 1939). Hydrogen is comprised of 99.9844% protium and 0.0156% deuterium, and oxygen is comprised of 99.763% oxygen-16, 0.0375% oxygen-17 and 0.1995% oxygen-18 (Hoefs 1973). It is extremely difficult to measure absolute abundance of stable isotopes, thus they are usually measured and expressed relative to a standard.

Keywords

Stable Isotope Isotope Ratio Hydrogen Isotope Carbon Isotope Ratio Cupric Oxide 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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References

  1. Alexander WJ, Mitchell RL (1949) Rapid measurement of cellulose viscosity by the nitration method. Anal Chem 21:1497–1500CrossRefGoogle Scholar
  2. Craig H (1961) Isotope variations in meteoric water. Science 133:1702–1703PubMedCrossRefGoogle Scholar
  3. DeNiro MJ (1981) The effects of different methods of preparing cellulose nitrate on the determination of the D/H ratios of non-exchangeable hydrogen of cellulose. Earth Planet Sci Lett 54:177–185CrossRefGoogle Scholar
  4. DeNiro MJ, Epstein S (1981) Isotopic composition of cellulose from aquatic organisms. Geochim Cosmochim Acta 42:495–506CrossRefGoogle Scholar
  5. Epstein S, Thompson P, Yapp CJ (1977) Oxygen and hydrogen isotopic ratios in plant cellulose. Science 198:209–1215CrossRefGoogle Scholar
  6. Estep MP, Hoering TC (1980) Biogeochemistry of the stable hydrogen isotopes. Geochim Cosmochim Acta 44:1197–1206CrossRefGoogle Scholar
  7. Friedman I (1953) Deuterium content of natural waters. Geochim Cosmochim Acta 4:89–103CrossRefGoogle Scholar
  8. Gat R, Gonfiantini R (1981) Stable isotope hydrology: deuterium and oxygen-18 in the water cycle. International Atomic Energy Agency, Vienna, Austria Technical Report No 206Google Scholar
  9. Hoefs J (1973) Stable isotope geochemistry. Springer, Berlin Heidelberg New YorkGoogle Scholar
  10. Libby LM, Pandolfi LF, Payton PH, Marshall J, Bercker B, Sienhenlist VG (1976) Isotopic thermometers. Nature 261:284–288CrossRefGoogle Scholar
  11. Nier AO, Gulbransen EA (1939) Variations in the relative abundance of the carbon isotopes. J Am Chem Soc 61:697–698CrossRefGoogle Scholar
  12. O’Leary MH (1981) Carbon isotope fractionation in plants. Phytochemistry 20:553–567CrossRefGoogle Scholar
  13. Schiegl WE (1972) Deuterium content of peat as a paleoclimatic recorder. Science 175:512–513PubMedCrossRefGoogle Scholar
  14. Sternberg L da SL (1988) Oxygen and hydrogen isotope ratios in plant cellulose: mechanisms and application. In: Ehleringer J, Rundel P, Nagy KA (eds) Stable Isotopes in Ecological Research. Springer, Berlin Heidelberg New York Tokyo (in press)Google Scholar
  15. Sternberg L, DeNiro MJ (1983) Isotopic composition of cellulose from C3, C4 and CAM plants growing in the vicinity of one another. Science 220:9947–948CrossRefGoogle Scholar
  16. Sternberg LO, DeNiro MJ, Ting IP (1984) Carbon, hydrogen and oxygen isotope ratios of cellulose from plants having intermediate photosynthetic modes. Plant Physiol 74:104–107PubMedCrossRefGoogle Scholar
  17. Timmel TE (1955) Chain-length distributions of native white spruce cellulose. Pulp Paper Mag Can 104–117Google Scholar
  18. Ting IP, Rayder L (1982) Regulation of C3 to CAM shifts. In: Ting IP, Gibbs M (eds) Cras- sulacean acid metabolism. Am Soc Plant Physiol, pp 677–679Google Scholar
  19. Wise LE (1944) Wood chemistry. Reinhold, Washington D.C., USAGoogle Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • L. Da Silveira Lobo Sternberg

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