Abstract
Respiration can be defined in the simplest of terms as the consumption of oxygen. In reality, it is a complex process involving chemical reactions in glycolysis and the Krebs cycle and electron transfer in the electron transport chains. The process of respiration is controlled by interactions at many levels, including organ, tissue, cell, organelle and pathway. In addition, there are many other causes of oxygen consumption. Despite these complexities, respiration is usually measured simply as oxygen consumption. The purpose of this chapter is to demonstrate how measurements of oxygen consumption can be used to study differences in respiration in plant roots throughout development. Techniques will be outlined for apportioning respiratory activity, determining the impact of tissue organization and accounting for the confounding effects of extramitochondrial oxygen consumption.
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References
Bahr JT, Bonner WD Jr (1973) Cyanide-insensitive respiration. I. The steady state of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem 248: 3441–3445
Bonner WD Jr, Slater EC (1970) Effect of antimycin on the potato mitochondrial cy-tochrome b system. Biochim Biophys Acta 223: 349–358
Boyer WD, Romancier RM, Ralston CW (1971) Root respiration rates of four tree species grown in the field. For Sci 17: 492–493
Burton WG (1950) Studies on the dormancy and sprouting of potatoes. I. The oxygen content of the potato tuber. New Phytol 49: 121–134
Clarkson DT, Robards AW (1975) The endodermis, its structural development and physiological role. In:Torrey JJ, Clarkson DT (eds) The development and function of roots. Academic Press, New York London, pp 415–436
Cole ME, Solomos T, Faust M (1982) Growth and respiration of dormant flower buds of Pyrus communis and Pyrus calleryana. J Am Soc Hort Sci 107: 226–231
Day DA, Arron GP, Laties GG (1980) Nature and control of respiratory pathways in plants:the interaction of cyanide-resistant respiration with the cyanide-sensitive pathway. In:Davies DD (ed) The biochemistry of plants. Academic Press, New York London, pp 197–241
Dizengremel P (1985) Potato respiration:electron transport pathways. In:Li P (ed) Potato physiology. Academic Press, New York London, pp 59–109
Goldstein AH, Anderson JO, McDaniel RG (1980) Cyanide-insensitive and cyanide-sensitive O2 uptake in wheat. Plant Physiol 66: 488–493
Goldstein AH, Anderson JO, McDaniel RG (1981) Cyanide-insensitive and cyanide-sensitive O2 uptake in wheat. II. Gradient-purified mitochondria lack cyanide-insensitive respiration. Plant Physiol 67: 594–596
Green DE, Mii S, Kohaut PM (1955) Studies on the terminal electron transport system. I. Succinic dehydrogenase. J Biol Chem 217: 551–556
Grover SD, Laties GG (1978) Characterization of the binding properties of disulfiram, an inhibitor of cyanide resistant respiration. In:Ducet G, Lance C (eds) Plant mitochondria. Elsevier New York, pp 259–266
Hansen GK (1976) Adaptation to photosynthesis and diurnal oscillations of root respiration rates for Lolium perenne. Physiol Plant 39: 275–279
Hausknecht EC, Funk MO (1984) The differential effect of disulfiram on lipoxygenases from Glycine max. Phytochemistry 23: 1535–1539
Huq S, Palmer JM (1978) Superoxide and hydrogen peroxide production in cyanide resistant Arum maculatum mitochondria. Plant Sci Lett 11: 351–358
Ikuma H, Bonner WD (1967) Properties of higher plant mitochondria. III. Effects of respiratory inhibitors. Plant Physiol 42: 1535–1544
James TW, Spencer MS (1979) Cyanide-insensitive respiration in pea cotyledons. Plant Physiol 64: 431–434
Janes HW, Chin CK (1981) The effect of age and growing conditions on cyanide resistance in culture tomato roots. Plant Sci Lett 23: 307–313
Jensen WA (1962) Botanical histochemistry. Freeman, San Francisco
Johnson-Flanagan AM (1980) Rotenone-resistant respiration in mitochondria isolated from etiolated pea cotyledons. Thesis, Univ Alberta, Edmonton
Johnson-Flanagan AM (1984) Growth and development of styroplug white spruce [Picea glauca (Moench) Voss] seedling roots. Thesis, Univ Victoria
Johnson-Flanagan AM, Owens JN (1985 a) Development of white spruce [Picea glauca (Moench) Voss] seedling roots. Plant Physiol 79: 103–107
Johnson-Flanagan AM, Owens JN (1985 b) Peroxidase activity in relation to suberization and respiration in white spruce [Picea glauca (Moench) Voss] seedling roots. Plant Physiol 79: 103–107
Johnson-Flanagan AM, Owens JN (1986) Root respiration in white spruce [Picea glauca (Moench) Voss] seedlings in relation to morphology and environment. Plant Physiol 81: 21–25
Johnson-Flanagan AM, Singh J (1986) Membrane deletion during plasmolysis in hardened and non-hardened plant cells. Plant Cell Environ 9: 299–305
Johnson-Flanagan AM, Spencer MS (1981) The effect of rotenone on respiration in pea cotyledon mitochondria. Plant Physiol 68: 1211–1217
Lahde E (1967) Studies on the respiration rate in the different parts of the roots systems of pine and spruce seedlings and its variation during the growth season. Acta For Fenn 81: 5–24
Lambers H (1980) The physiological significance of cyanide-resistant respiration in higher plants. Plant Cell Environ 3: 293–302
Lambers HS, van de Dijk SJ (1979) Cyanide-resistant root respiration and tap root formation in two subspecies of Hypochaeris radiata. Physiol Plant 45: 235–239
Lambers H, Steingröver E (1978) Efficiency of root respiration of a flood-tolerant and flood-intolerant Senecio species as affected by low oxygen tension. Physiol Plant 42: 179–184
Lambers H, Blacquiere T, Stuiver B (CEE) (1981) Interactions between osmoregulation and the alternative respiratory pathway in Plant ago coronopus as affected by salinity. Physiol Plant 51: 63–68
Laties GG (1974) Isolation of mitochondria from plant material. In:Fleischer S, Packer L (eds) Methods of enzymology, vol 31. Biomembranes. Academic Press, New York, pp 589–606
Ledig FT, Drew AP, Clark JG (1976) Maintenance and constructive respiration, photosynthesis and net assimilation rate in seedlings of pitch pine (Pinus rigida Mill). Ann Bot 40: 289–300
Loomis WD (1974) Overcoming problems of phenolics and quinones in the isolation of plant membranes and organelles. In:Fleischer S, Packer L (eds) Methods of enzymology, vol 31. Biomembranes. Academic Press, New York, pp 528–553
Loughman BC (1960) Uptake and utilization of phosphate associated with respiratory changes in potato tubes slices. Plant Physiol 35: 418–424
Miller MG, Obendorf RL (1981) Use of tetraethylthiuram disulphide to discriminate between alternative respiration and lipoxygenase. Plant Physiol 67: 962–964
Neuburger M, Journet EP, Bligny R, Carde JP, Douce R (1982) Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch Biochem Biophys 217: 312–323
Palmer JM (1976) The organization and regulation of electron transport in mitochondria. Annu Rev Plant Physiol 27: 133–157
Parrish DJ, Leopold AC (1977) Transient changes during soybean inhibition. Plant Physiol 59: 1111–1115
Parrish DJ, Leopold AC (1978) Confounding of alternative respiration by lipoxygenase activity. Plant Physiol 62: 470–472
Peterson CA, Edgington LV (1975) Uptake of the systemic fungicide methylo-2-benzimid-azolecarbamate and the fluorescent dye PTS by onion roots. Phytopathology 65: 1254–1259
Peterson CA, Emanuel ME, Humphreys GB (1981) Pathway of the movement of apoplas-tic fluorescent dye tracers through the endodermis at the site of secondary root formation in corn (Zea mays) and broad bean (Vicia faba). Can J Bot 59: 618–625
Rich PR, Weigand NK, Blum H, Moore AL, Bonner WD Jr. (1978) Studies on the mechanism of inhibition of redox enzymes by substituted hydroxamic acids. Biochim Bio-phys Acta 525: 325–337
Shingles RM, Arron GP, Hill RD (1982) Alternative pathway respiration and lipoxygenase activity in aged potato slice mitochondria. Plant Physiol 69: 1435–1438
Siedow JN, Girvin ME (1980) Alternative respiratory pathway. Its role in seed respiration and its inhibition by propyl gallate. Plant Physiol 65: 669–674
Slater EC (1969) Application of inhibitors and uncouplers for a study of oxidative phos-phorylation. In:Estabrook RW, Pullman ME (eds) Methods of enzymology, vol 10. Academic Press, New York, pp 48–56
Solomos T, Malhotra SS, Prasad S, Malhotra AK, Spencer M (1972) Biochemical and structural changes in mitochondria and other cellular components of pea cotyledons during germination. Can J Biochem 50: 725–737
Steingröver E (1981) The relationship between cyanide-resistant root respiration and the storage of sugars in the taproot in Daucus carota L. J Exp Bot 32: 911–919
Szaniawski RK (1981) Growth and maintenance respiration of shoots and roots in Scots pine seedlings. Z Pflanzenphysiol 101: 391–398
Tappel AL (1961) Biocatalysts:lipoxidase and hematin compounds. In:Lundberg WO (ed) Autoxidation and antioxidants, vol 1. Wiley, New York, pp 325–366
Theologis A, Laties GG (1978 a) Relative contribution of cytochrome-mediated and cyanide-resistant electron transport in fresh and aged potato slices. Plant Physiol 62: 232–237
Theologis A, Laties GG (1978 b) Antimycin-insensitive cytochrome-mediated respiration in fresh and aged potato slices. Plant Physiol 62: 238–242
Theologis A, Laties GG (1980) Membrane lipid breakdown in relation to wound-induced and cyanide-resistant respiration in tissue slices. Plant Physiol 66: 690–896
Webb T, Armstrong W (1983) Effects of KCN and salicylhydroxamic acid on the root respiration of pea seedlings. Plant Physiol 72: 280–286
Wiskich JT (1980) Controls of the Krebs cycle. In:Davies DD (ed) The biochemistry of plants, vol 2. Academic Press, New York London, pp 243–278
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Johnson-Flanagan, A.M. (1989). Respiration Measurements in Plant Roots Throughout Development. In: Linskens, HF., Jackson, J.F. (eds) Gases in Plant and Microbial Cells. Modern Methods of Plant Analysis, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83346-5_6
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DOI: https://doi.org/10.1007/978-3-642-83346-5_6
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-83348-9
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