Respiration Measurements in Plant Roots Throughout Development

  • A. M. Johnson-Flanagan
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 9)

Abstract

Respiration can be defined in the simplest of terms as the consumption of oxygen. In reality, it is a complex process involving chemical reactions in glycolysis and the Krebs cycle and electron transfer in the electron transport chains. The process of respiration is controlled by interactions at many levels, including organ, tissue, cell, organelle and pathway. In addition, there are many other causes of oxygen consumption. Despite these complexities, respiration is usually measured simply as oxygen consumption. The purpose of this chapter is to demonstrate how measurements of oxygen consumption can be used to study differences in respiration in plant roots throughout development. Techniques will be outlined for apportioning respiratory activity, determining the impact of tissue organization and accounting for the confounding effects of extramitochondrial oxygen consumption.

Keywords

Surfactant Sucrose Starch Cysteine Polysaccharide 

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References

  1. Bahr JT, Bonner WD Jr (1973) Cyanide-insensitive respiration. I. The steady state of skunk cabbage spadix and bean hypocotyl mitochondria. J Biol Chem 248: 3441–3445Google Scholar
  2. Bonner WD Jr, Slater EC (1970) Effect of antimycin on the potato mitochondrial cy-tochrome b system. Biochim Biophys Acta 223: 349–358PubMedCrossRefGoogle Scholar
  3. Boyer WD, Romancier RM, Ralston CW (1971) Root respiration rates of four tree species grown in the field. For Sci 17: 492–493Google Scholar
  4. Burton WG (1950) Studies on the dormancy and sprouting of potatoes. I. The oxygen content of the potato tuber. New Phytol 49: 121–134Google Scholar
  5. Clarkson DT, Robards AW (1975) The endodermis, its structural development and physiological role. In:Torrey JJ, Clarkson DT (eds) The development and function of roots. Academic Press, New York London, pp 415–436Google Scholar
  6. Cole ME, Solomos T, Faust M (1982) Growth and respiration of dormant flower buds of Pyrus communis and Pyrus calleryana. J Am Soc Hort Sci 107: 226–231Google Scholar
  7. Day DA, Arron GP, Laties GG (1980) Nature and control of respiratory pathways in plants:the interaction of cyanide-resistant respiration with the cyanide-sensitive pathway. In:Davies DD (ed) The biochemistry of plants. Academic Press, New York London, pp 197–241Google Scholar
  8. Dizengremel P (1985) Potato respiration:electron transport pathways. In:Li P (ed) Potato physiology. Academic Press, New York London, pp 59–109Google Scholar
  9. Goldstein AH, Anderson JO, McDaniel RG (1980) Cyanide-insensitive and cyanide-sensitive O2 uptake in wheat. Plant Physiol 66: 488–493PubMedCrossRefGoogle Scholar
  10. Goldstein AH, Anderson JO, McDaniel RG (1981) Cyanide-insensitive and cyanide-sensitive O2 uptake in wheat. II. Gradient-purified mitochondria lack cyanide-insensitive respiration. Plant Physiol 67: 594–596Google Scholar
  11. Green DE, Mii S, Kohaut PM (1955) Studies on the terminal electron transport system. I. Succinic dehydrogenase. J Biol Chem 217: 551–556Google Scholar
  12. Grover SD, Laties GG (1978) Characterization of the binding properties of disulfiram, an inhibitor of cyanide resistant respiration. In:Ducet G, Lance C (eds) Plant mitochondria. Elsevier New York, pp 259–266Google Scholar
  13. Hansen GK (1976) Adaptation to photosynthesis and diurnal oscillations of root respiration rates for Lolium perenne. Physiol Plant 39: 275–279CrossRefGoogle Scholar
  14. Hausknecht EC, Funk MO (1984) The differential effect of disulfiram on lipoxygenases from Glycine max. Phytochemistry 23: 1535–1539CrossRefGoogle Scholar
  15. Huq S, Palmer JM (1978) Superoxide and hydrogen peroxide production in cyanide resistant Arum maculatum mitochondria. Plant Sci Lett 11: 351–358CrossRefGoogle Scholar
  16. Ikuma H, Bonner WD (1967) Properties of higher plant mitochondria. III. Effects of respiratory inhibitors. Plant Physiol 42: 1535–1544Google Scholar
  17. James TW, Spencer MS (1979) Cyanide-insensitive respiration in pea cotyledons. Plant Physiol 64: 431–434PubMedCrossRefGoogle Scholar
  18. Janes HW, Chin CK (1981) The effect of age and growing conditions on cyanide resistance in culture tomato roots. Plant Sci Lett 23: 307–313CrossRefGoogle Scholar
  19. Jensen WA (1962) Botanical histochemistry. Freeman, San FranciscoGoogle Scholar
  20. Johnson-Flanagan AM (1980) Rotenone-resistant respiration in mitochondria isolated from etiolated pea cotyledons. Thesis, Univ Alberta, EdmontonGoogle Scholar
  21. Johnson-Flanagan AM (1984) Growth and development of styroplug white spruce [Picea glauca (Moench) Voss] seedling roots. Thesis, Univ VictoriaGoogle Scholar
  22. Johnson-Flanagan AM, Owens JN (1985 a) Development of white spruce [Picea glauca (Moench) Voss] seedling roots. Plant Physiol 79: 103–107PubMedCrossRefGoogle Scholar
  23. Johnson-Flanagan AM, Owens JN (1985 b) Peroxidase activity in relation to suberization and respiration in white spruce [Picea glauca (Moench) Voss] seedling roots. Plant Physiol 79: 103–107PubMedCrossRefGoogle Scholar
  24. Johnson-Flanagan AM, Owens JN (1986) Root respiration in white spruce [Picea glauca (Moench) Voss] seedlings in relation to morphology and environment. Plant Physiol 81: 21–25PubMedCrossRefGoogle Scholar
  25. Johnson-Flanagan AM, Singh J (1986) Membrane deletion during plasmolysis in hardened and non-hardened plant cells. Plant Cell Environ 9: 299–305Google Scholar
  26. Johnson-Flanagan AM, Spencer MS (1981) The effect of rotenone on respiration in pea cotyledon mitochondria. Plant Physiol 68: 1211–1217PubMedCrossRefGoogle Scholar
  27. Lahde E (1967) Studies on the respiration rate in the different parts of the roots systems of pine and spruce seedlings and its variation during the growth season. Acta For Fenn 81: 5–24Google Scholar
  28. Lambers H (1980) The physiological significance of cyanide-resistant respiration in higher plants. Plant Cell Environ 3: 293–302CrossRefGoogle Scholar
  29. Lambers HS, van de Dijk SJ (1979) Cyanide-resistant root respiration and tap root formation in two subspecies of Hypochaeris radiata. Physiol Plant 45: 235–239CrossRefGoogle Scholar
  30. Lambers H, Steingröver E (1978) Efficiency of root respiration of a flood-tolerant and flood-intolerant Senecio species as affected by low oxygen tension. Physiol Plant 42: 179–184CrossRefGoogle Scholar
  31. Lambers H, Blacquiere T, Stuiver B (CEE) (1981) Interactions between osmoregulation and the alternative respiratory pathway in Plant ago coronopus as affected by salinity. Physiol Plant 51: 63–68CrossRefGoogle Scholar
  32. Laties GG (1974) Isolation of mitochondria from plant material. In:Fleischer S, Packer L (eds) Methods of enzymology, vol 31. Biomembranes. Academic Press, New York, pp 589–606Google Scholar
  33. Ledig FT, Drew AP, Clark JG (1976) Maintenance and constructive respiration, photosynthesis and net assimilation rate in seedlings of pitch pine (Pinus rigida Mill). Ann Bot 40: 289–300Google Scholar
  34. Loomis WD (1974) Overcoming problems of phenolics and quinones in the isolation of plant membranes and organelles. In:Fleischer S, Packer L (eds) Methods of enzymology, vol 31. Biomembranes. Academic Press, New York, pp 528–553Google Scholar
  35. Loughman BC (1960) Uptake and utilization of phosphate associated with respiratory changes in potato tubes slices. Plant Physiol 35: 418–424PubMedCrossRefGoogle Scholar
  36. Miller MG, Obendorf RL (1981) Use of tetraethylthiuram disulphide to discriminate between alternative respiration and lipoxygenase. Plant Physiol 67: 962–964PubMedCrossRefGoogle Scholar
  37. Neuburger M, Journet EP, Bligny R, Carde JP, Douce R (1982) Purification of plant mitochondria by isopycnic centrifugation in density gradients of Percoll. Arch Biochem Biophys 217: 312–323PubMedCrossRefGoogle Scholar
  38. Palmer JM (1976) The organization and regulation of electron transport in mitochondria. Annu Rev Plant Physiol 27: 133–157CrossRefGoogle Scholar
  39. Parrish DJ, Leopold AC (1977) Transient changes during soybean inhibition. Plant Physiol 59: 1111–1115PubMedCrossRefGoogle Scholar
  40. Parrish DJ, Leopold AC (1978) Confounding of alternative respiration by lipoxygenase activity. Plant Physiol 62: 470–472PubMedCrossRefGoogle Scholar
  41. Peterson CA, Edgington LV (1975) Uptake of the systemic fungicide methylo-2-benzimid-azolecarbamate and the fluorescent dye PTS by onion roots. Phytopathology 65: 1254–1259CrossRefGoogle Scholar
  42. Peterson CA, Emanuel ME, Humphreys GB (1981) Pathway of the movement of apoplas-tic fluorescent dye tracers through the endodermis at the site of secondary root formation in corn (Zea mays) and broad bean (Vicia faba). Can J Bot 59: 618–625CrossRefGoogle Scholar
  43. Rich PR, Weigand NK, Blum H, Moore AL, Bonner WD Jr. (1978) Studies on the mechanism of inhibition of redox enzymes by substituted hydroxamic acids. Biochim Bio-phys Acta 525: 325–337Google Scholar
  44. Shingles RM, Arron GP, Hill RD (1982) Alternative pathway respiration and lipoxygenase activity in aged potato slice mitochondria. Plant Physiol 69: 1435–1438PubMedCrossRefGoogle Scholar
  45. Siedow JN, Girvin ME (1980) Alternative respiratory pathway. Its role in seed respiration and its inhibition by propyl gallate. Plant Physiol 65: 669–674Google Scholar
  46. Slater EC (1969) Application of inhibitors and uncouplers for a study of oxidative phos-phorylation. In:Estabrook RW, Pullman ME (eds) Methods of enzymology, vol 10. Academic Press, New York, pp 48–56Google Scholar
  47. Solomos T, Malhotra SS, Prasad S, Malhotra AK, Spencer M (1972) Biochemical and structural changes in mitochondria and other cellular components of pea cotyledons during germination. Can J Biochem 50: 725–737PubMedCrossRefGoogle Scholar
  48. Steingröver E (1981) The relationship between cyanide-resistant root respiration and the storage of sugars in the taproot in Daucus carota L. J Exp Bot 32: 911–919CrossRefGoogle Scholar
  49. Szaniawski RK (1981) Growth and maintenance respiration of shoots and roots in Scots pine seedlings. Z Pflanzenphysiol 101: 391–398Google Scholar
  50. Tappel AL (1961) Biocatalysts:lipoxidase and hematin compounds. In:Lundberg WO (ed) Autoxidation and antioxidants, vol 1. Wiley, New York, pp 325–366Google Scholar
  51. Theologis A, Laties GG (1978 a) Relative contribution of cytochrome-mediated and cyanide-resistant electron transport in fresh and aged potato slices. Plant Physiol 62: 232–237PubMedCrossRefGoogle Scholar
  52. Theologis A, Laties GG (1978 b) Antimycin-insensitive cytochrome-mediated respiration in fresh and aged potato slices. Plant Physiol 62: 238–242PubMedCrossRefGoogle Scholar
  53. Theologis A, Laties GG (1980) Membrane lipid breakdown in relation to wound-induced and cyanide-resistant respiration in tissue slices. Plant Physiol 66: 690–896CrossRefGoogle Scholar
  54. Webb T, Armstrong W (1983) Effects of KCN and salicylhydroxamic acid on the root respiration of pea seedlings. Plant Physiol 72: 280–286PubMedCrossRefGoogle Scholar
  55. Wiskich JT (1980) Controls of the Krebs cycle. In:Davies DD (ed) The biochemistry of plants, vol 2. Academic Press, New York London, pp 243–278Google Scholar

Copyright information

© Springer-Verlag Berlin Heidelberg 1989

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  • A. M. Johnson-Flanagan

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