Skip to main content

Analytical Gas Exchange Measurements of Photosynthetic CO2 Assimilation

  • Chapter
Gases in Plant and Microbial Cells

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 9))

Abstract

Carbon dioxide is assimilated by plants in the process of photosynthesis. Virtually all life depends on this process. The measurement of carbon dioxide assimilation is one method for studying photosynthesis which is adaptable both to reductionist questions such as what specific biochemical process limits the overall rate of photosynthesis and to ecophysiological questions such as how does water stress affect photosynthesis. The techniques required by reductionists and ecophysiologists are similar and are the subject of this chapter. Other descriptions of these methods will also be useful (Bloom et al. 1980; Field et al. 1982 and in press; Ball 1987; Field and Mooney in press).

This is a preview of subscription content, log in via an institution to check access.

Access this chapter

eBook
USD 16.99
Price excludes VAT (USA)
  • Available as PDF
  • Read on any device
  • Instant download
  • Own it forever
Softcover Book
USD 109.99
Price excludes VAT (USA)
  • Compact, lightweight edition
  • Dispatched in 3 to 5 business days
  • Free shipping worldwide - see info

Tax calculation will be finalised at checkout

Purchases are for personal use only

Institutional subscriptions

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  • Badger MR, Sharkey TD, Caemmerer S von (1984) The relationship between steady-state gas exchange of bean leaves and the levels of carbon-reduction-cycle intermediates. Planta 160: 305–313

    Article  CAS  Google Scholar 

  • Ball JT (1987) Calculations related to gas exchange. In:Zeiger E, Farquhar GD, Cowan IR (eds) Stomatal function. Stanford Univ Press, Stanford, pp 445–476

    Google Scholar 

  • Bloom AJ, Mooney HA, Björkman O, Berry J (1980) Materials and methods for carbon dioxide and water exchange analysis. Plant Cell Environ 3: 371–376

    Article  CAS  Google Scholar 

  • Brooks A, Farquhar GD (1985) Effects of temperature on the O2/CO2 specificity of ribu-lose-l,5-bisphosphate carboxylase/oxygenase and the rate of respiration in the light. Estimates from gas exchange measurement on spinach. Planta 165: 397–406

    CAS  Google Scholar 

  • Caemmerer S von, Edmondson DL (1986) The relationship between steady-state gas exchange, in vivo RuP2 carboxylase activity and some carbon reduction cycle intermediates in Raphanus sativus. Aust J Plant Physiol 13: 669–688

    Article  Google Scholar 

  • Caemmerer S von, Farquhar GD (1981) Some relationships between the biochemistry of photosynthesis and the gas exchange of leaves. Planta 153: 376–387

    Article  Google Scholar 

  • Caemmerer S von, Farquhar GD (1984) Effects of partial defoliation, changes of irradiance during growth, short-term water stress and growth at enhanced p(CO2) on the photo-synthetic capacity of leaves of Phaseolus vulgaris L. Planta 160: 320–329

    Article  Google Scholar 

  • Farquhar GD, Caemmerer S von, Berry JA (1980) A biochemical model of photosynthetic CO2 assimilation in leaves of C3 species. Planta 149: 78–90

    Google Scholar 

  • Field CB, Mooney HA (in press) Measuring photosynthesis under field conditions — past and present approaches. In:Kramer PJ, Strain BR, Funada S, Hashimoto Y (eds) Scientific instruments in physiological plant ecology. Academic Press, New York London

    Google Scholar 

  • Field C, Berry JA, Mooney HA (1982) A portable system for measuring carbon dioxide and water vapour exchange of leaves. Plant Cell Environ 5: 179–186

    Google Scholar 

  • Field CB, Ball JT, Berry JA (in press) Photosynthesis:principles and field techniques. In:Pearcy RW, Ehlringer J, Mooney HA, Rundel P (eds) Physiological plant ecology. Field methods and instrumentation

    Google Scholar 

  • Gaastra P (1959) Photosynthesis of crop plants as influenced by light, carbon dioxide, temperature, and stomatal diffusion resistance. Meded Landbouwhogesch Wageningen 59(13): 1–68

    Google Scholar 

  • Hardwick RC, Cole RA, Fyfield TP (1984) Injury to and death of cabbage (Brassica ol-eracea) seedlings caused by vapours of dibutylphthalate emitted from certain plastics. Ann Appl Biol 105: 97–105

    Article  CAS  Google Scholar 

  • Hill DW, Powell T (1968) Non-dispersive infrared gas analysis in science, medicine and industry. Plenum, New York

    Google Scholar 

  • Jarman PD (1974) The diffusion of carbon dioxide and water vapour through stomata. J Exp Bot 25: 927–936

    Article  Google Scholar 

  • Laisk A (1977) Kinetics of photosynthesis and photorespiration of C3 plants. Nauka, Moscow (in Russian)

    Google Scholar 

  • Leunig R (1983) Transport of gases into leaves. Plant Cell Environ 6: 181–194

    Google Scholar 

  • Moss DN, Rawlins SL (1963) Concentration of carbon dioxide inside leaves. Nature 197: 1320–1321

    Article  CAS  Google Scholar 

  • Mott KA, O’Leary JW 81984) Stomatal behavior and CO2 exchange characteristics in am-phistomatous leaves. Plant Physiol 74: 47–51

    Google Scholar 

  • Mott KA, Jensen RG, O’Leary JW, Berry JA (1984) Photosynthesis and ribulose 1,5-bis-phosphate concentrations in intact leaves of Xanthium strumarium L. Plant Physiol 76: 968–971

    Article  PubMed  CAS  Google Scholar 

  • Parkinson KJ, Day W (1981) Water vapour calibration using salt hydrate transitions. J Exp Bot 32: 411–418

    Article  CAS  Google Scholar 

  • Penman H1, Schofield RK (1951) Some physical aspects of assimilation and transpiration. Symp Soc Exp Biol 5: 115–129

    Google Scholar 

  • Seemann JR, Sharkey TD (1986) Salinity and nitrogen effects on photosynthesis, ribulose-f 1,5-bisphosphate carboxylase and metabolite pool sizes in Phaseolus vulgaris L. Plant Physiol 82: 555–560

    Article  PubMed  CAS  Google Scholar 

  • Seemann JR, Sharkey TD (1987) The effect of abscisic acid and other inhibitors on photo-synthetic capacity and the biochemistry of CO2 assimilation. Plant Physiol 84: 696–700

    Article  PubMed  CAS  Google Scholar 

  • Seemann JR, Tepperman JM, Berry JA (1981) The relationship between photosynthetic performance and the levels and kinetic properties of RuBP carboxylase-oxygenase from desert winter annuals. Carnegie Inst Washington Yearb 80: 67–72

    Google Scholar 

  • Sestak Z, Catsky J, Jarvis PG (1971) Plant photosynthetic production manual of methods. Junk, The Hague

    Google Scholar 

  • Sharkey TD (1984) Transpiration-induced changes in the photosynthetic capacity of leaves. Plant Physiol 160: 143–150

    Google Scholar 

  • Sharkey TD (1985) Photosynthesis in intact leaves of C3 plants:physics, physiology and rate limitations. Bot Rev 51: 53–105

    Article  Google Scholar 

  • Sharkey TD, Imai K, Farquhar GD, Cowan IR (1982) A direct conformation of the standard method of estimating intercellular partial pressure of CO2. Plant Physiol 69: 657–659

    Article  PubMed  CAS  Google Scholar 

  • Sharkey TD, Seemann JR, Berry JA (1986a) Regulation of ribulose-1,5-bisphosphate car-boxlase activity in response to changing partial pressure of O2 and light in Phaseolus vulgaris. Plant Physiol 81: 788–791

    Article  PubMed  CAS  Google Scholar 

  • Sharkey TD, Stitt M, Heineke D, Gerhardt R, Raschke K, Heldt HW (1986 b) Limitation of photosynthesis by carbon metabolism II. O2-insensitive CO2 uptake results from limitation of triose phosphate utilization. Plant Physiol 81: 1123–1129

    Article  PubMed  CAS  Google Scholar 

Download references

Authors

Editor information

Editors and Affiliations

Rights and permissions

Reprints and permissions

Copyright information

© 1989 Springer-Verlag Berlin Heidelberg

About this chapter

Cite this chapter

Sharkey, T.D. (1989). Analytical Gas Exchange Measurements of Photosynthetic CO2 Assimilation. In: Linskens, HF., Jackson, J.F. (eds) Gases in Plant and Microbial Cells. Modern Methods of Plant Analysis, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83346-5_5

Download citation

  • DOI: https://doi.org/10.1007/978-3-642-83346-5_5

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83348-9

  • Online ISBN: 978-3-642-83346-5

  • eBook Packages: Springer Book Archive

Publish with us

Policies and ethics