O2 Exchange Measurement Using a Platinum Polarographic Electrode

  • W. Vidaver
  • S. Swenson
Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 9)


The platinum polarographic electrode has been used extensively to study O2 exchange in photosynthetic organisms for several decades (Haxo and Blinks 1950; Myers and Graham 1963; Blinks 1964; Joliot and Joliot 1968; Joliot et al. 1970; Kok et al. 1970; Chandler and Vidaver 1971; Forbush et al. 1971; Delieu and Walker 1981; Swenson et al. 1986). One of the earliest applications of the platinum electrode resulted in photosynthetic action spectra for various aquatic and marine algae that remain as definitive examples of qualitative light-use efficiency by plants (Haxo and Blinks 1950). Subsequent studies have utilized polarographic techniques to investigate every conceivable aspect of O2 evolution or uptake in intact macroorganisms (Chandler and Vidaver 1970; Weiss and Sauer 1970; Swenson et al. 1986), cell suspensions or single cells (Joliot 1968; Ried 1968; Delrieu 1972; Greenbaum and Mauzerall 1976; Diner 1977; Jursinic 1981; Delrieu 1983 a), isolated chloroplasts (Fork 1963 b; Joliot and Joliot 1968; Kok et al. 1970; Schmid and Thibault 1979; Wydrzynski and Sauer 1980; Delrieu 1984; Sinclair 1984; see Inoue, this Vol.), thylakoid preparations (Yamaoka et al. 1978; Bader et al. 1983; Vermaas et al. 1984; Tang and Satoh 1985) and submembrane fractions of the photosynthetic apparatus, such as Photosystem II (PS II) particles (Lavorel and Scibert 1982; Clement-Metral and Gantt 1983; Cohen and Barton 1983; Wensink et al. 1984; Ikeuchi et al. 1985; Cole et al. 1986).


Oxygen Evolution Exchange Measurement Electrode System Bare Electrode Photosynthetic Oxygen Evolution 
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© Springer-Verlag Berlin Heidelberg 1989

Authors and Affiliations

  • W. Vidaver
  • S. Swenson

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