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Methods for Uptake and Assimilation Studies of Nitrogen Dioxide

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Gases in Plant and Microbial Cells

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 9))

Abstract

Nitrogen dioxide is a major air pollutant, discharged into the environment from both natural and anthropogenic sources, although its background concentration in most localities is well below the concentrations reported to produce visible damage and death of plants (National Academy of Sciences 1971). The control of industrial NO2 emissions to the atmosphere has lagged behind that of other pollutants such as SO2. Several scientists have suggested the creation of green belts in urban and sub-urban areas to reduce the air pollution load, as plants have been found to be an efficient sink of air pollutants including NO2 (Hill 1971). The pollutants can diffuse into plant tissues through open stomata, as do CO2,02 and water vapour, although cuticular absorption also accounts for some pollutant removal from the atmosphere. Plants play a major role in regulating the concentration of CO2 and O2 in the air; they may also play an important role in removing air pollution.

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References

  • Anderson LS, Mansfield TA (1979) The effect of nitric oxide pollution on the growth of tomato. Environ Pollut 20: 113–121

    Article  CAS  Google Scholar 

  • AOAC (1975) Official methods of analysis of the association of the official agricultural chemists, 9th edn. Herwitz W (ed). AOAC, Washington, DC, pp 1094

    Google Scholar 

  • Brunetti N, Hageman RH (1976) Comparison of in vivo and in vitro assays of nitrate re-ductase in wheat (Triticum aestivum). Plant Physiol 58: 583–587

    Article  PubMed  CAS  Google Scholar 

  • Colowick SP, Kaplan NO (1980) Photosynthesis and nitrogen fixation. In:San Pietro A (ed) Methods in enzymology, vol 69. Academic Press, New York London, p 894

    Google Scholar 

  • Decormis L, Bonte J, Tisne A (1975) Technique expérimentale permettant l’étude de l’incidence sur la végétation d’une pollution par le dioxide de soufre appliqué en permanence et à dose subnecrotique. Pollut Atmosph 17: 103–107

    Google Scholar 

  • Dee LA, Martens HH, Merill CI, Nakamura JT, Jaye FC (1973) New manual method for nitrogen dioxide emission measurement. Anal Chem 45: 1477–1481

    Article  PubMed  CAS  Google Scholar 

  • Freer-Smith PH (1985) The influence of SO2 and NO2 on the growth, development and gas exchange of Betula pendula Roth. New Phytol 99: 417–430

    Article  CAS  Google Scholar 

  • Fuhrer J, Erismann KH (1980) Uptake of NO2 by plants grown at different salinity levels. Experientia 36: 409–410

    Article  PubMed  CAS  Google Scholar 

  • Heagle AS, Body DE, Heck WW (1973) An open top field chamber to assess the impact of air pollution on plants. J Environ Qual 2: 365–368

    Article  CAS  Google Scholar 

  • Heck WW, Dunning JA, Johnson H (1968) Design of a simple plant exposure chamber. DHEW, National Center for Air Pollution Control Publications APTD-68-6 Washington, DC

    Google Scholar 

  • Heck WW, Philbeck RB, Dunning JA (1978) A continuous stirred tank reactor (CSTR) system for exposing plants to gaseous air contaminants; principles, specifications, construction and operation. US Agric Res Ser s-181

    Google Scholar 

  • Hill AC (1971) Vegetation:a sink for atmospheric pollutants. J Air Pollut Control Assoc 21: 341–346

    PubMed  CAS  Google Scholar 

  • Ito K, Okano K, Kuroina M, Totsuka T (1985) Effects of NO2 and O3 alone and in combination on kidney bean plants (Phaseolus vulgaris L.):growth, partitioning of assimilates and root activities. J Exp Bot 36: 652–662

    Article  CAS  Google Scholar 

  • Jefferies HE, Rodgers HH, Stahel EP (1976) Spatially uniform environment for the dynamic study of biological systems; application of the continuous stirred tank reactor concept. Sci Biol 2: 180–182

    Google Scholar 

  • Lee JJ, Lewis RA (1977) Zonal air pollution system. Design and performance. In:Preston EM, Lewis RA (eds) The bioenvironmental impact of a coal fired power plant. 3rd Interim Rep EPA-600/3-78-021, pp 322–344

    Google Scholar 

  • Maclean DC, McCune DC, Weinstein LH, Mandl RH, Woodruff GN (1968) Effects of acute hydrogen fluoride and nitrogen dioxide exposures on Citrus and ornamental plants of central Florida. Environ Sci Technol 2: 444–449

    Article  CAS  Google Scholar 

  • Mandl RH, Weinstein LH, McCune DC, Keveny M (1973) A cylindrical open top chamber for the exposure of plants to air pollutants in the field. J Environ Qual 2: 372–376

    Article  Google Scholar 

  • Matsumaru T, Yoneyama T, Totsuka T, Shiratori K (1979) Absorption of atmospheric NO2 by plants and soils. I. Quantitative estimation of absorbed NO2 in plants by 15N methods. Soil Sci Plant Nutrit 25: 255–265

    CAS  Google Scholar 

  • Matsumaru T, Yoneyama T, Totsuka T, Mutsuoka Y (1981) Absorption of atmospheric NO2 by rice, wheat and barley plants. Estimation by the 15N method. Soil Sci Plant Nutrit 27: 255–261

    CAS  Google Scholar 

  • Matsushima J (1972) Influence of S02 and N02 on assimilation of amino acids, organic acids and saccharoid in Citrus nutsuidaidi seedlings. Bull Fac Agric Mie Univ 44: 131–139

    Google Scholar 

  • Muller RN, Miller JE, Sprugel DG (1979) Photosynthetic response of field grown soybeans to fumigation with sulfur dioxide. J Appl Ecol 16: 567–576

    Article  CAS  Google Scholar 

  • National Academy of Sciences (ed) (1971) Nitrogen oxides. Natl Acad Sci Washington, DC, pp 333

    Google Scholar 

  • Okano K, Totsuka T (1986) Absorption of nitrogen dioxide by sunflower plants grown at various levels of nitrate. New Phytol 102: 551–562

    Article  CAS  Google Scholar 

  • Okano K, Fukuzawa T, Tazaki T, Totsuka T (1986) 15N dilution method for estimating the absorption of atmospheric NO2 by plants. New Phytol 102: 73–84

    Article  CAS  Google Scholar 

  • Oleksyn J (1984) Effects of SO2, HF and NO2 on net photosynthetic and dark respiration rates of Scots pine needles of various ages. Photosynthetica 18: 259–262

    CAS  Google Scholar 

  • Preiss MC, Woodin SJ, Lee JA (1986) The potential importance of an increased atmospheric nitrogen supply to the growth of ombrotrophic Sphagnum species. New Phytol 103: 45–55

    Article  Google Scholar 

  • Rogers HH, Aneja VP (1980) Uptake of atmospheric ammonia by selected plant species. Environ Exp Bot 20: 251–257

    Article  CAS  Google Scholar 

  • Rogers HH, Jeffries HE, Stahel EP, Heck WW, Ripperton LA, Witherspoon AM (1977) Measuring air pollutant uptake by plants:a direct kinetic technique. J Air Pollut Control Assoc 27: 1192–1197

    CAS  Google Scholar 

  • Rogers HH, Campbell JC, Volk JC (1979 a) Nitrogen-15 dioxide uptake and incorporation by Phaseolus vulgaris L. Science 206: 333–335

    Article  PubMed  CAS  Google Scholar 

  • Rogers HH, Jeffries HE, Witherspoon AM (1979 b) Measuring air pollutant uptake by plants:nitrogen dioxide. J Environ Qual 88: 551–557

    Article  Google Scholar 

  • Rowland AJ (1986) Nitrogen uptake, assimilation and transport in barley in the presence of atmospheric nitrogen dioxide. Plant Soil 91: 353–356

    Article  Google Scholar 

  • Saltzman BE (1954) Colorimetric microdetermination of nitrogen dioxide in the atmosphere. Anal Chem 26: 1949–1955

    Article  CAS  Google Scholar 

  • Sinn JP, Pell EJ (1984) Impact of chronic nitrogen dioxide exposures on composition and yield of potato foliage and tubers. J Am Soc Hortic Sci 109: 481–484

    CAS  Google Scholar 

  • Sinn JP, Pell EJ, Decaria A (1984) A NO2 generating system for use in air pollution research. Penn Agric Exp Stn Prog Rep 382

    Google Scholar 

  • Spierings FHFG (1971) Influence of fumigation with NO2 on growth and yield of tomato plants. Neth J Plant Pathol 77: 194–200

    Article  CAS  Google Scholar 

  • Srivastava HS (1974) Effects of NO2 on gas exchange in bean leaves. Ph D Thesis, Univ British Columbia, Vancouver

    Google Scholar 

  • Srivastava HS (1980) Regulation of nitrate reductase activity in higher plants. Phyto-chemistry 19: 725–733

    CAS  Google Scholar 

  • Srivastava HS, Ormrod DP (1984) Effects of nitrogen dioxide and nitrate nutrition on growth and nitrate assimilation in bean leaves. Plant Physiol 76: 418–423

    Article  PubMed  CAS  Google Scholar 

  • Srivastava HS, Ormrod DP (1986) Effects of nitrogen dioxide and nitrate nutrition on nodulation, nitrogenase activity, growth and nitrogen content of bean plants. Plant Physiol 81: 737–741

    Article  PubMed  CAS  Google Scholar 

  • Srivastava HS, Singh RP (1987) Role and regulation of 1-glutamate dehydrogenase activity in higher plants. Phytochemistry 26: 597–610

    Article  CAS  Google Scholar 

  • Srivastava HS, Jolliffe PA, Runeckles VC (1975 a) Inhibition of gas exchange in bean leaves by NO2. Can J Bot 53: 466–474

    Article  CAS  Google Scholar 

  • Srivastava HS, Jolliffe PA, Runeckles VC (1975 b) The effects of environmental conditions on the inhibition of leaf gas exchange by NO2. Can J Bot 53: 475–482

    Article  CAS  Google Scholar 

  • Takeuchi Y, Nihira J, Kondo N, Tezuka T (1985) Changes in nitrate reducing activity in squash seedlings with NO2 fumigation. Plant Cell Physiol 26: 1027–1035

    CAS  Google Scholar 

  • Troiano JJ, Leone IA (1977) Changes in growth rate and nitrogen content of tomato plants after exposure to NO2. Phytopathology 67: 1130–1133

    Article  CAS  Google Scholar 

  • Unsworth MH (1982) Exposure to gaseous pollutants and uptake by plants. In:Unsworth MH, Ormrod DP (eds) Effects of gaseous air pollution in agriculture and horticulture. Butterworth, London, p 43

    Google Scholar 

  • Wellburn AR, Higginson C, Robinson D, Walmsley C (1981) Biochemical explanation of more than additive inhibitory effects of low atmospheric levels of sulfur dioxide plus nitrogen dioxide upon plants. New Phytol 88: 223–237

    Article  CAS  Google Scholar 

  • Yoneyama T, Sasakawa H (1979) Transformation of atmospheric NO2 absorbed in spinach leaves. Plant Cell Physiol 20: 263–266

    CAS  Google Scholar 

  • Yoneyama T, Sasakawa H, Ishizuka S, Totsuka T (1979) Absorption of atmospheric NO2 by plants and soils, II:Nitrite accumulation, nitrite reductase activity and diurnal changes of NO2 absorption in leaves. Soil Sci Plant Nutrit 25: 267–275

    CAS  Google Scholar 

  • Yoneyama T, Arai K, Totsuka T (1980) Transfer of nitrogen and carbon from a mature sunflower leaf-15NO2 and 13CO2 feeding studies. Plant Cell Physiol 21: 1367–1381

    CAS  Google Scholar 

  • Zeevaart AJ (1974) Induction of nitrate reductase by NO2. Acta Bot Neerl 23: 345–346

    Google Scholar 

  • Zeevaart AJ (1976) Some effects of fumigating plants for short periods with NO2. Environ Pollut 11: 98–108

    Google Scholar 

Download references

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© 1989 Springer-Verlag Berlin Heidelberg

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Srivastava, H.S., Ormrod, D.P., Marie, B.H. (1989). Methods for Uptake and Assimilation Studies of Nitrogen Dioxide. In: Linskens, HF., Jackson, J.F. (eds) Gases in Plant and Microbial Cells. Modern Methods of Plant Analysis, vol 9. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83346-5_12

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  • DOI: https://doi.org/10.1007/978-3-642-83346-5_12

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-83348-9

  • Online ISBN: 978-3-642-83346-5

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