The Enzyme-Linked Immunosorbent Assay (ELISA) in the Beverage Industries: Principles and Practice

Part of the Modern Methods of Plant Analysis book series (MOLMETHPLANT, volume 8)


The analytical techniques compiled under the name Enzyme-Linked ImmunoSor-bent Assay, ELISA, provide highly sensitive and specific means for measurement of a variety of compounds, if only antibodies to the substance of interest can be obtained. Antibodies themselves may also be quantitated by ELISA procedures. The name enzyme-linked immunosorbent assay was introduced by Engvall and Perlmann (1971, 1972), and, at the same time, similar procedures were described by van Weemen and Schuurs (1971). Since the initial descriptions, different varieties of the basic procedures have been described (reviewed by, among others, Voller et al. 1979; O’Sullivan et al. 1979 a; Schuurs and van Weemen 1980; Blake and Gould 1984).


Enzyme Immunoassay Immunol Method Beverage Industry Barley Flour ELISA Procedure 
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