Effect of Hyperthermia at 40.5° C and Cytostatic Drugs on Human Bone Marrow Progenitor Cells
Hyperthermia in clinical treatment is mostly used locally, either alone or in combination with radio- or chemotherapy. In the treatment of local tumors, this modality is of proven therapeutic benefit (Cavaliere et al. 1982; Dewhirst et al. 1983). A small group of clinicians, however, have tried to apply whole body hyperthermia. Their therapeutic aim is the thermal enhancement of cytostatic drug effects in the treatment of disseminated malignancies (Pettigrew et al. 1974; Barlogie et al. 1979; Bull et al. 1979; Parks et al. 1979; Neumann et al. 1982). In using this modality, it is of critical importance to know whether hyperthermia can damage normal bone marrow and, if enhancement of cytostatic drug effects is indeed achieved, whether the action might be not only on the tumor cells but also on the bone marrow cells. It was therefore the aim of our study to establish whether hyperthermia can enhance the cytotoxicity of several cytostatic drugs on normal human bone marrow cells. For the clinical application of whole body hyperthermia the temperatures and the treatment times must be restricted to a tolerable level. We therefore used a temperature of 40.5° C for 2 h.
KeywordsBone Marrow Cell Colony Formation Mouse Bone Marrow Normal Bone Marrow Cytostatic Drug
Unable to display preview. Download preview PDF.
- Aye MT, Niho Y, Till JE, McCulloch EA (1977) Studies of leukemic cell populations in culture. Blood 44: 205–214Google Scholar
- Balogie B, Corry PM, Yip E, Lippmann I, Johnston DA, Khalil K, Tenczynski TF, Reilly E, Lawson R, Dosik G, Rigor B, Hankenson R, Freireich EJ (1979) Total-body hyperthermia with and without chemotherapy for advanced neoplasms. Cancer Res 39: 1481–1489Google Scholar
- Cavaliere R, Di Filippo F, Moricca G, Santori F, Varahese A, Pantano FP, Cassanelli A, Aloe L, Monticelli G (1982) Hyperthermic perfusion for treatment of tumors of the extremities. Chemotherapy 1: 278–287Google Scholar
- Hahn GM (1983) Hyperthermia to enhance drug delivery. In: Chabner BA (ed) Rational basis for chemotherapy, UCLA symp on molecular and cellular biology-new series, vol.4. UCLA symposium on the rational basis for chemotherapy, Keystone, CO 1982. Liss, New York, pp 427–436Google Scholar
- Neumann HA, Fabricius HA, Engelhardt R (1982) Moderate whole-body hyperthermia in combination with chemotherapy in the treatment of small cell carcinoma of the lung: a pilot study. Natl Cancer Inst Monogr 61: 427–429Google Scholar
- O’Donnel JF, McKoy WS, Makuch RW, Bull JM (1979) Increased in vitro toxicity to mouse bone marrow with 1,3-bis (2-chloroethyl) 1-nitrosourea and hyperthermia. Cancer Res 39: 1547–2549Google Scholar