Abstract
A rapid in vitro assay based on morphological changes in the nucleus has, recently, been reported for predicting human cancer chemosensitivity (Tokita et al. 1986 a, b; Takamizawa et al. 1985). This method provides a realistic potential with regard to the following advantages: (1) We can apply it to any kind of tumors, including intestinal tumors contaminated with bacteria. Consequently, the proportion of tumors yielding sensitivity data is high. (2) The results are obtained within 4 h for alkylating agents and Vinca alkaloids, within 8 h for antibiotic and antimetabolic agents. (3) The assay can be done in a clinical laboratory on a regular basis because of its simplicity.
This study was supported by Bundesministerium für Jugend, Familie, Frauen und Gesundheit, Bonn.
This is a preview of subscription content, log in via an institution to check access.
Access this chapter
Tax calculation will be finalised at checkout
Purchases are for personal use only
Preview
Unable to display preview. Download preview PDF.
References
Andrysek O, Bláhová E, Gregora V, Rezney Z (1985) An experimental model for predicting the synergism of hyperthermia with cytostatics. Neoplasma 32: 93–101
Arcangeli G, Dividalli A, Nervi C, Creton G (1983) Tumor control and therapeutic gain with different schedules of combined radiotherapy and local external hyperthermia in human cancer. Int J Radiat Oncol Biol Phys 9:1125–1134
Fajardo LF, Egbert B, Marmor J, Hahn GM (1980) Effects of hyperthermia in a malignant tumor. Cancer 45: 613–623
Hamburger AW, Salmon SE (1977) Primary bioassay of human tumors stem cells. Science 197: 461–463
Kim JH, Hahn EW, Ahmed SA (1982) Combination of hyperthermia and radiation for malignant melanoma. Cancer 50: 478–482
Mann BD, Kern DH, Giuliano AE, Burk MW, Campbell MA, Kaiser LR, Morton DL (1982) Clinical correlation with drug sensitivities in the clonogenic assay. Arch Surg 117: 33–36
Mann BD, Storm FK, Morton DL, Bertelsen CA, Korn EL, Kaiser LR, Kern DH (1983) Predictability of response to clinical thermochemotherapy by the clonogenic assay. Cancer 52: 1389–1394
Neumann HA, Fiebig HH, Lohr GW, Engelhardt R (1985) Effects of cytostatic drugs and 40.5° C hyperthermia on human bone marrow progenitors (CFU-C) and human clonogenic tumor cells implanted into mice. JNCI 75: 1059–1066
Overgaard J (1976) Influence of extracellular pH on the viability and morphology of tumor cells exposed to hyperthermia. JNCI 56:1243–1250
Salmon SE, Albert DS, Durie BGM, Meysken FL, Soehnlen B, Chen HS, Moon T (1980) Clinical correlation of drug sensitivity in the human tumor stem cell assay. Recent Results Cancer Res 74: 300–305
Salmon SE, Meysken FL, Albert DS (1981) New drug in ovarian cancer and malignant melanoma: in vitro phase II screening with the human tumor stem cell assay. Cancer Treat Rep 65:1–12
Scott RS, Johnson RJ, Kowal H, Krisnamsetty RM, Story K, Clay L (1983) Hyperthermia in combination with radiotherapy: a review of five years experience in the treatment of superficial tumors. Int J Radiat Oncol Biol Phys 9:1327–1333
Stehlin JS (1980)Hyperthermic perfusion for melanoma of the extremities: experience with 165 patients, 1967 to 1979. Ann NY Acad Sci 335: 352–355
Storm FK, Sillberman AW, Ramming KR, Kaiser LR, Harrison WH, Elliot RS, Haskel CM, Sarna G, Morton DL (1984) Clinical thermochemotherapy: a control trial in advanced cancer patient. Cancer 53: 863–868
Takamizawa H, Sekiya S, Iwamisawa H, Ishige H, Tokita H, Tanaka N (1985) A new in vitro chemosensitivity test: individualized chemotherapy against ovarian cancer and its clinical effect. Gan To Kagaku Ryoho 12: 2293–2297
Tokita H, Tanaka N, Ueno T (1982) Morphological changes of tumor cells by antitumor drugs -comparison of in vivo and in vitro. 41st Japanese Cancer Congress, Kyoto
Tokita H, Tanaka N, Fujimoto S, Nakao K (1986 a) The in vitro cytotoxic test for predicting human colon and rectum cancer chemosensitivity. Med Biol 112: 171–175
Tokita H, Tanaka N, Ueno T, Fujimoto S, Nakao K, Takada N, Takamisawa H (1986b) In vitro cytotoxic test for predicting human cancer chemosensitivity (Abstr 3883). 14th International Cancer Congress, Budapest
Van der Zee J, van Rhoon GC, Wike-Hooley JL, Faithful NS, Rheinhold HS (1983) Whole-body hyperthermia in cancer therapy: a report of a phase I-II study. Eur J Cancer Clin Oncol 19: 1189–1200
Von HoffDD (1983) Send this patient’s tumor for culture and sensitivity. N Engl J Med 308: 154–155
Von HoffDD, Clark GM, Stogdill BJ, Sarosdy J, O’Brien MT, Casper JT, Mattox DE, Page CP, Cruz AB, Sandbach JF(1983) Prospective clinical trial of a human tumor cloning system. Cancer Res 43: 1926–1931
Yamada K, Neumann HA, Fiebig HH, Engelhardt R, Tokita H (1988) A rapid in vitro assay for predicting human cancer thermochemosensitivity. Recent Results Cancer Res (in press)
Author information
Authors and Affiliations
Editor information
Editors and Affiliations
Rights and permissions
Copyright information
© 1988 Springer-Verlag Berlin · Heidelberg
About this paper
Cite this paper
Yamada, K., Neumann, H.A., Fiebig, H.H., Engelhardt, R., Tokita, H. (1988). A Rapid in Vitro Assay for Predicting Thermochemosensitivity of Human Cancer: Comparison with Clonogenic Assay. In: Issels, R.D., Wilmanns, W. (eds) Application of Hyperthermia in the Treatment of Cancer. Recent Results in Cancer Research, vol 107. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-83260-4_25
Download citation
DOI: https://doi.org/10.1007/978-3-642-83260-4_25
Publisher Name: Springer, Berlin, Heidelberg
Print ISBN: 978-3-642-83262-8
Online ISBN: 978-3-642-83260-4
eBook Packages: Springer Book Archive