Growth, Cell Proliferation and Morphological Alterations of a Mouse Mammary Carcinoma After Exposure to X-Rays and Hyperthermia

  • K. C. George
  • D.van Beuningen
  • C. Streffer
Part of the Recent Results in Cancer Research book series (RECENTCANCER, volume 107)


There are numerous studies on the effects of radiation and hyperthermia on experimental animal tumours (Denekamp and Fowler 1977; Hahn 1982). Tumour regression is the criterion of response frequently used in most of these studies. However, a number of biological parameters can influence the effects of radiation and hyperthermic treatment on tumours, and only few of these studies consider simultaneously tumour growth, tumour cell proliferation and morphological alterations (Thomlinson and Craddock 1967; Hermens and Barendsen 1969; Kovacs et al. 1976; Nelson et al. 1976; Rowley et al. 1980). Therefore, it was of interest to study several such parameters on the same tumour system. Metabolic studies were carried out by our group with a C57 mouse mammary carcinoma. The tumours were irradiated with one of various doses of X-rays and heated to 43° C for 30 min following exposure to 10 Gy. Tumour growth, cell proliferation, formation of micronuclei and morphological changes in necrosis and density of small blood vessels were studied.


Morphological Alteration Small Blood Vessel Tumour Core Mouse Mammary Carcinoma Relative Tumour Volume 
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  1. Denekamp J, Fowler JF (1977)Cell proliferation kinetics and radiation therapy. In: Becker FF. (ed) Cancer:A comprehensive treatise, vol.6. Plenum, New York, pp 101–137Google Scholar
  2. Hahn GM (1982) Hyperthermia and cancer. Plenum, New YorkCrossRefGoogle Scholar
  3. Hermens AF, Barendsen GW (1978) The proliferation status and clonogenic capacity of tumour cells in a transplantable rhabdomyosarcoma of the rat before and after irradiation with 800 rad of X-rays. Cell Tissue Kinet 11: 83PubMedGoogle Scholar
  4. Hilmas DE, Gillette EL (1975) Microvasculature of C3H/Bi mouse mammary tumours after X-irradiation. Radiat Res 61: 128–143PubMedCrossRefGoogle Scholar
  5. Kovacs CJ, Hopkins HA, Evans MJ, Looney WB (1976) Changes in cellularity induced by radiation in a solid tumour. Int J Radiat Biol 30: 101–113CrossRefGoogle Scholar
  6. Nelson JSR, Carpenter RE, Burboraw RE (1976) Mechanisms underlying reduced growth rate in C3H BA mammary adenocarcinoma recurring after single doses of X-rays or fast neutrons. Cancer Res 36:524–531PubMedGoogle Scholar
  7. Reinhold HS, Wike-Hooley JC, van den Berg AP, van den Berg Blok A (1982)Environmental factors, blood flow and microcirculation. In: Overgaard J. (ed) Hyperthermic oncology. Taylor and Francis, London, pp 41–52Google Scholar
  8. Rowley R, Hopkins HA, Bestill WL, Retnour ER, Looney WB (1980) Response and recovery kinetics of a solid tumour after irradiation. Br J Cancer 42: 586–595PubMedCrossRefGoogle Scholar
  9. Streffer C, van Beuningen D, Bamberg M, Eigler FW, Gross E, Schabronath J (1984) An approach to the individualization of cancer therapy - determination of DNA, SH groups and micronuclei.Strahlentherapie 160: 661–666PubMedGoogle Scholar
  10. Thomlinson RH, Craddock EA (1967) The gross response of an experimental tumour to single doses of X-rays. Br J Cancer 21: 108–123PubMedCrossRefGoogle Scholar
  11. Walter J, Maurer-Schultze B (1987) Regrowth, tumour cell proliferation and morphological alterations of the adenocarcinoma E0771 following a single dose of 30 Gy 60Co 7-rays. Strahlentherapie (in press)Google Scholar

Copyright information

© Springer-Verlag Berlin · Heidelberg 1988

Authors and Affiliations

  • K. C. George
    • 1
  • D.van Beuningen
    • 1
  • C. Streffer
    • 1
  1. 1.Institut für Medizinische StrahlenbiologieUniversitätsklinikum EssenEssen 1Germany

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