The Role of PDGF in Health and Disease

Conference paper
Part of the Veröffentlichungen aus der Geomedizinischen Forschungsstelle der Heidelberger Akademie der Wissenschaften book series (HD AKAD, volume 1987/88 / 1987/4)

Abstract

Platelet-derived growth factor (PDGF), originally recognized as the principal source of mitogenic activity present in whole blood serum and missing in plasma or plasma-derived serum, has now been shown to be derived from many diploid cells as well as a number of tumor cells. Although the platelet was the first cell of origin to be discovered, it is clear that PDGF is stored in alpha granules in the platelet and is synthesized and stored in these granules in the megakaryocyte. Since the purification and characterization of platelet-derived growth factor has been accomplished by a number of different laboratories, several assays have been developed that permit the determination of other cells that can make and secrete PDGF. The assays that are routinely used in our laboratory include: 1) a receptor competition binding assay based on the fact that PDGF binds with high affinity (10-11M) to a specific cell-surface receptor. This competition binding assay, therefore, measures the capacity of unknowns containing PDGF or PDGF-like substances to compete for binding to this receptor. 2) An ELISA was established based on the development of a monospecific polyclonal anti-PDGF IgG that was developed in a goat. This antibody can completely abolish the mitogenic activity of highly purified PDGF.

Keywords

Catheter Arthritis Sarcoma 

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Copyright information

© Springer-Verlag Berlin Heidelberg 1987

Authors and Affiliations

  • R. Ross
    • 1
  1. 1.Department of Pathology, SM-30, School of MedicineUniversity of WashingtonSeattleUSA

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