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Methods for Computer Analysis and Comparison of Two-Dimensional Protein Patterns Obtained by Electrophoresis

  • Reinhold C. Mann
  • Betty K. Mansfield
  • James K. Selkirk
Part of the NATO ASI Series book series (volume 30)

Abstract

Mammalian cells contain thousands of proteins each representing an important function for maintaining the homeostasis of the cell at the biochemical, genetic, or structural level. Two-dimensional gel electrophoresis is uniquely sensitive in its ability to separate these proteins in the first dimension by isoelectric focusing as a function of net charge, and in the second dimension as a function of molecular weight ([13]). The presence of a certain radioactively labeled protein in a preparation is indicated by a spot that is produced on a film. The intensity of the spot is indicative of the amount of protein. Usually several hundred, even up to a few thousand spots, i.e., proteins, can be distinguished in a gel image, which makes rigorous quantitative analysis by simple visual inspection impossible. Analysis of the voluminous information inherent in a gel image requires localization, quantitation and possibly identification of each protein. In addition, and equally importantly, it is necessary to make local and global comparisons between multiple gels that reflect potential changes in protein patterns from various disease states or after carcinogen or mutagen treatment, in order to demonstrate subtle changes that occur in the progression to the diseased state.

Keywords

Projection Pursuit Simple Visual Inspection Spot Profile Projection Pursuit Method Rigorous Quantitative Analysis 
These keywords were added by machine and not by the authors. This process is experimental and the keywords may be updated as the learning algorithm improves.

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Copyright information

© Springer-verlag Berlin Heidelberg 1987

Authors and Affiliations

  • Reinhold C. Mann
    • 1
  • Betty K. Mansfield
    • 1
  • James K. Selkirk
    • 2
  1. 1.Biology DivisionOak Ridge National LaboratoryOak RidgeUSA
  2. 2.National Toxicology ProgramNIEHSResearch Triangle ParkUSA

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