High Performance Liquid Chromatography of Phytoalexins
Phytoalexins have been defined as “low molecular weight, antimicrobial compounds that are both synthesized by and accumulated in plants after exposure to microorganisms” (Paxton 1981). They have been studied from many points of view; as natural products, as chemotaxonomic markers, as antimicrobial agents, and as the indirect products of gene derepression. Investigations of the mechanisms by which their accumulation is triggered (or, to use the accepted terminology, elicited) are numerous and considerable attention has also been paid to both biosynthesis and degradation. Above all, it is the putative role of phytoalexins in the defence of plants against microbial attack which provides the most important stimulus for their study. Recent work in this area has been encouraging and before long we may find plant breeders, in their quest for resistance, wishing to select lines with a high capacity for phytoalexin synthesis. If this does come about the requirement for speedy methods of qualitative and quantitative analysis will be enormous.
KeywordsEthyl Acetate Fraction Film Evaporation Soybean Cotyledon Microbial Challenge Phytoalexin Accumulation
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