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The Determination of Abscisic Acid by High Performance Liquid Chromatography

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High Performance Liquid Chromatography in Plant Sciences

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 5))

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Abstract

In the last 10 years, high performance liquid chromatography (HPLC) has largely replaced the older forms of liquid chromatography, gravity column and thin layer chromatography as the method of choice for separating plant hormones. Apologists for HPLC point out the shorter separation times, higher resolution, and greater convenience of HPLC compared to alternative methods. While the advantages of HPLC are veritable, they are not always realized. Separating abscisic acid (ABA) from a plant extract by HPLC is relatively easy, but achieving separations of high quality may prove difficult.

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Authors
  1. J. M. Robertson
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Editors and Affiliations

  1. Goldberglein 7, D-8520, Erlangen, Germany

    Hans-Ferdinand Linskens

  2. Department of Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, S.A., Australia

    John F. Jackson

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© 1987 Springer-Verlag Berlin Heidelberg

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Robertson, J.M. (1987). The Determination of Abscisic Acid by High Performance Liquid Chromatography. In: Linskens, HF., Jackson, J.F. (eds) High Performance Liquid Chromatography in Plant Sciences. Modern Methods of Plant Analysis, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82951-2_4

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  • DOI: https://doi.org/10.1007/978-3-642-82951-2_4

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-82953-6

  • Online ISBN: 978-3-642-82951-2

  • eBook Packages: Springer Book Archive

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