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Separation and Purification of Abscisic Acid and Its Catabolites by High Performance Liquid Chromatography

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High Performance Liquid Chromatography in Plant Sciences

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 5))

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Abstract

Numerous theories on the role of ABA as a plant growth regulator have been proposed, including, but not limited to, root gravitropism (Wilkins 1978, 1984; but see Moore and Evans 1986), embryo and bud dormancy (Addicott and Lyon 1969; Walton 1980; Wareing 1978), and stomatal closure (Raschke 1975). However, Wareing (1978) and Walton (1980) have concluded that too little is known about the events occurring during any physiological process to state unequivocally that ABA is involved in its control. The best evidence, although still correlative, for the involvement of ABA in a physiological process has been obtained with experiments on water stress. Detached or attached leaves will accumulate ABA (usually 10 to 40 times that found in a turgid leaf) upon the imposition of water stress. When ABA is given to epidermal strips or fed to intact leaves through the transpiration stream, stomata close rapidly (Raschke 1975; Davies and Mansfield 1983). The effect of ABA on stomata is rather rapid compared to its effect on protein synthesis, as in the control of α-amylase production in barley aleurone layers. In this tissue ABA inhibits the gibberellin-induced synthesis of α-amylase, which is needed for the hydrolysis of endosperm starch during germination (Ho 1983, Jacobsen 1983).

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Abbreviations

ABA-GE:

β-D-glucopyranosyl abscisate

ABA-GS:

1’-O-abscisic acid-β-D-glucopyranoside

DPA:

dihydrophaseic acid

DPA-GS:

4’-O-dihydrophaseic acid-β-D-glucopyranoside

EI:

electron impact

EIA:

enzyme immunoassay

GLC-ECD:

gas liquid chromatography-electron capture detection

GC-MS:

gas chromatography-mass spectroscopy

8’-OH-ABA:

8’-hydroxy ABA

HMG-ABA:

β-hydroxy-β-methylglutarylhydroxy abscisic acid

HPLC:

high performance liquid chromatography

min:

minute(s)

Me-ABA:

methyl ester of abscisic acid

NCI:

negative chemical ionization

ORD:

optical rotary dispersion

PA:

phaseic acid

PA-GE:

β-D-glucopyranosyl phaseate

RIA:

radioimmunoassay

SIM:

selected ion monitoring

TLC:

thin layer chromatography

UV:

ultraviolet

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Authors
  1. R. A. Creelman
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  2. J. A. D. Zeevaart
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Editor information

Editors and Affiliations

  1. Goldberglein 7, D-8520, Erlangen, Germany

    Hans-Ferdinand Linskens

  2. Department of Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, S.A., Australia

    John F. Jackson

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Creelman, R.A., Zeevaart, J.A.D. (1987). Separation and Purification of Abscisic Acid and Its Catabolites by High Performance Liquid Chromatography. In: Linskens, HF., Jackson, J.F. (eds) High Performance Liquid Chromatography in Plant Sciences. Modern Methods of Plant Analysis, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82951-2_3

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  • DOI: https://doi.org/10.1007/978-3-642-82951-2_3

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-82953-6

  • Online ISBN: 978-3-642-82951-2

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