Analysis of Lipids by High Performance Liquid Chromatography
High performance liquid chromatography (HPLC) of natural lipids has been remarkably slow to develop as an analytical tool. The absence of easily detectable functional groups on most lipids makes them difficult to measure quantitatively using the more common types of detectors. As a result, many of the published methods for underivatized lipid HPLC are semiquantitative at best and are primarily designed to separate rather than quantify the individual components. The structures of some lipids are amenable to derivatization, yielding light-absorbing products that may be quantified on a molar basis. Applications of this type have been quite successful, although extreme care must be taken to avoid degradation or selective losses of some lipid species during the derivatization steps.
KeywordsChloroform Acetonitrile Boron Lipase Nitrile
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