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High Performance Liquid Chromatography of Gibberellins

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High Performance Liquid Chromatography in Plant Sciences

Part of the book series: Modern Methods of Plant Analysis ((MOLMETHPLANT,volume 5))

Abstract

The ability to identify and accurately measure plant hormones has become increasingly important in studies on the physiology and biochemistry of plant hormones. The analysis of plant hormones such as the gibberellins in plant extracts poses many technical problems, primarily because only minute amounts are present. Their concentration in fresh material rarely exceeds 1 part in 106, but is often as low as 1 part in 1010 and therefore they require analytical procedures which are able to measure ng or rather pg quantities of hormone present in extracts. In addition, plant extracts contain many more low molecular compounds than animal tissue, and these are difficult to separate from the low molecular plant hormones. Thus plant hormone analysis, and that of gibberellins in particular, is not yet a routine procedure, and at the present time not one particular method can be singled out as the best way to analyze plant hormones.

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References

  • Akiyoshi DE, Morris RO, Hink R, Mischle BS, Kosuge T, Garfunkel DJ, Gordon MP, Nester EW (1983) Cytokinin/auxin balance in grown gall tumors is regulated by specific loci in the T-DNA. Proc Natl Acad Sci USA 80: 407–411

    Article  PubMed  CAS  Google Scholar 

  • Barendse GWM, Van der Werken PH, Takahashi N (1980) High performance liquid chromatography of gibberellins. J Chromatogr 198: 449–455

    Article  CAS  Google Scholar 

  • Barendse GWM, Dijkstra A, Moore TC (1983) The biosynthesis of the gibberellin precursor ent-kaurene in cell-free extracts and the endogenous gibberellins of Japanese Morning Glory in relation to seed development. J Plant Growth Regul 2: 165–175

    Article  CAS  Google Scholar 

  • Binks R, MacMillan J, Pryce RC (1969) Plant hormones VII. Combined gas chromatography-mass spectrometry of the methyl esters of gibberellins Al to A24 and their trimethylsilyl esters. Phytochemistry (Oxf) 8: 271–284

    Article  CAS  Google Scholar 

  • Brenner ML (1981) Modern methods for plant growth substance analysis. Annu Rev Plant Physiol 32: 511–538

    Article  CAS  Google Scholar 

  • Crozier A, Aoki H, Pharis RP (1969) Efficiency of counter-current distribution. Sephadex G-10 and silicic acid partition chromatography in the purification and separation of gibberellin-like substances from plant tissue. J Exp Bot 20: 786–795

    Article  CAS  Google Scholar 

  • Crozier A, Durley RC (1983) Modern methods of analysis of gibberellins. In: Crozier A (ed) The biochemistry and physiology of gibberellins, vol 1. Praeger, Eastbourne, East Sussex, pp 485–560

    Google Scholar 

  • Crozier A, Reeve DR (1977) The application of high performance liquid chromatography to the analysis of plant hormones. In: Pilet PE (ed) Proc 9th. Int Conf Plant Growth Subst. Springer, Berlin Heidelberg New York, pp 67–76

    Google Scholar 

  • Crozier A, Zaerr JB, Morris RO (1980) High-performance steric exclusion chromatography of plant hormones. J Chromatogr 198: 57–63

    Article  CAS  Google Scholar 

  • Crozier A, Zaerr JB, Morris RO (1982) Reversed-and normal-phase high-performance liquid chromatography of gibberellin methoxycoumaryl esters. J Chromatogr 238: 157–166

    Article  CAS  Google Scholar 

  • Davies JR, Jensen E, Rivier L, Juntilla O, Crozier A: Detection of gibberellins in extracts from Salix pentandra shoots by high performance liquid chromatography and combined gas chromatography-mass spectrometry (cited after Crozier and Durley 1983 )

    Google Scholar 

  • Dünges W (1977) 4-Bromomethyl-7-methoxycoumarin as a new fluorescent label for fatty acids. Anal Chem 49:442–445

    Google Scholar 

  • Durst D, Milano M, Katka GJ, Connely SA, Grushka E (1975) Phenacyl esters of fatty acids via crown ether catalysts for enhanced ultraviolet detection in liquid chromatography. Anal Chem 47: 1797–1801

    Article  PubMed  CAS  Google Scholar 

  • Glenn JL, Kuo CC, Durley RC, Pharis RP (1972) Use of insoluble polyvinyl pyrrolidone for purification of plant extracts and chromatography of plant hormones. Phytochemistry (Oxf) 11: 345–351

    Article  CAS  Google Scholar 

  • Gräbner R, Schneider G, Sembdner G (1976) Gibberelline. 43ste Mitt. Fraktionierung von gibberellinen, gibberellinkonjugaten und anderen phytohormonen durch DEAE-Sephadex-chromatography. J Chromatogr 121: 110–115

    Google Scholar 

  • Heftmann E, Saunders GA, Haddon WF (1978) Argentation high-pressure liquid chromatography and mass spectrometry of gibberellin esters. J Chromatogr 156: 71–77

    Article  CAS  Google Scholar 

  • Hiraga K, Yokota T, Murofushi N, Takahashi N (1974) Isolation and chracterization of gibberellins in mature seeds of Phaseolus vulgaris. Agric Biol Chem 38: 2511–2520

    Article  CAS  Google Scholar 

  • Jones MG, Metzger JD, Zeevaart JAD (1980) Fractionation of gibberellins in plant extract by reverse phase high performance liquid chromatography. Plant Physiol (Bethesda) 65: 218–221

    Article  CAS  Google Scholar 

  • Knox JH (1979) High performance liquid chromatography. Edinburgh Univ Press, Edinburgh

    Google Scholar 

  • Koshioka M, Harada J, Takerro K, Noma M, Sassa T, Ogiyama K, Taylor JS, Road SB, Zegge RL, Pharis RP (1983) Reversed phase C18 high pressure/performance liquid chromatography of acidic and conjugated gibberellins. J Chromatogr 256: 101–115

    Article  CAS  Google Scholar 

  • Lin J-T, Heftmann E (1981) Adsorption and reversed-phase partition high-performance liquid chromatography of gibberellins. J Chromatogr 213: 507–510

    Article  CAS  Google Scholar 

  • MacDonald EMS, Akiyoshi DE, Morris RO (1981) Combined high-performance liquid chromatography-radioimmuno assay for cytokinins. J Chromatogr 214: 101–109

    Article  CAS  Google Scholar 

  • Metzger JD, Zeevaart JAD (1980) Effect of photoperiod on the levels of endogenous gibberellins in spinach as measured by combined gas chromatography-selected ion current monitoring. Plant Physiol (Bethesda) 66: 844–846

    Article  CAS  Google Scholar 

  • Metzger JD, Zeevaart JAD (1982) Photoperiodic control of gibberellin metabolism in spinach. Plant Physiol (Bethesda) 69: 287–291

    Article  CAS  Google Scholar 

  • Morris RO, Zaerr JB (1978) 4-Bromophenocyl esters of gibberellins, useful derivatives for high performance liquid chromatography. Anal Lett A11:73–83

    Google Scholar 

  • Reeve DR, Crozier A (1976) Purification of plant hormone extracts by gel permeation chromatography. Phytochemistry (Oxf) 15: 791–793

    Article  CAS  Google Scholar 

  • Reeve DR, Crozier A (1978) The analysis of gibberellins by high performance liquid chromatography. In: Hillman JR (ed) Isolation of plant growth substances. Cambridge Univ Press, London, pp 41–78

    Google Scholar 

  • Reeve DR, Crozier A (1980) Quantitative analysis of plant hormones. In: MacMillan J (ed) Hormonal regulation of development I. Molecular aspects of plant hormones. Springer, Berlin Heidelberg New York, pp 203–280

    Google Scholar 

  • Reeve DR, Yokota T, Nask LJ, Crozier A (1976) The development of a high performance liquid chromatograph with a sensitive on-stream radioactivity monitor for the analysis of 3H and 14C labelled gibberellins. J Exp Bot 27: 1243–1258

    Article  CAS  Google Scholar 

  • Takahashi N, Yamaguchi I, Yamane H (1986) Gibberellins. In: Takahashi (ed) Chemistry of plant hormones. CRC Press Inc., Boca Raton, Florida, pp 282, (ISBN–0–8493–54706)

    Google Scholar 

  • Yabuta T, Hayashi T (1939) Biochemical studies on “bakanae” fungus of the rice II. Isolation of gibberellin, the active principles which makes the rice seedings grow slenderly. J Agric Chem Soc Jpn 15: 257–266

    Google Scholar 

  • Yamaguchi I, Yokota T, Yoshida S, Takahashi N (1979) High pressure liquid chromatography of conjugated gibberellins. Phytochemistry (Oxf) 18: 1699–1702

    Article  CAS  Google Scholar 

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Authors
  1. G. W. M. Barendse
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Editors and Affiliations

  1. Goldberglein 7, D-8520, Erlangen, Germany

    Hans-Ferdinand Linskens

  2. Department of Biochemistry, Waite Agricultural Research Institute, University of Adelaide, 5064, Glen Osmond, S.A., Australia

    John F. Jackson

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© 1987 Springer-Verlag Berlin Heidelberg

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Barendse, G.W.M. (1987). High Performance Liquid Chromatography of Gibberellins. In: Linskens, HF., Jackson, J.F. (eds) High Performance Liquid Chromatography in Plant Sciences. Modern Methods of Plant Analysis, vol 5. Springer, Berlin, Heidelberg. https://doi.org/10.1007/978-3-642-82951-2_1

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  • DOI: https://doi.org/10.1007/978-3-642-82951-2_1

  • Publisher Name: Springer, Berlin, Heidelberg

  • Print ISBN: 978-3-642-82953-6

  • Online ISBN: 978-3-642-82951-2

  • eBook Packages: Springer Book Archive

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